1887

Abstract

Methane-producing bacterial strain MS2(T = type strain) was isolated from a tannery by-products enrichment culture inoculated with sewage sludge. This methanogen was a non-motile, irregular coccoid organism (diameter, 1 to 2 μm) which used H-CO and formate as methanogenic substrates. Acetate was required for growth but did not serve as a methanogenic substrate; yeast extract and Trypticase peptone were highly stimulatory. Growth occurred through the pH range from 5.5 to 8.0, with optimum growth at pH 6.7. The optimum temperature for growth was 37°C. The deoxyribonucleic acid base composition was 59 mol% guanine plus cytosine. The physiological and antigenic properties of this isolate place it in the genus The organism is named sp. nov.

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1986-04-01
2024-04-24
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References

  1. Balch W. E., Fox G. E., Magrum L. J., Wolfe R. S. 1979; Methanogens: réévaluation of a unique biological group. Microbiol. Rev. 43:260–296
    [Google Scholar]
  2. Baresi L., Mah R. A., Ward D. M., Kaplan I. R. 1978; Methanogenesis from acetate: enrichment studies. Appl. Environ. Microbiol. 36:186–197
    [Google Scholar]
  3. Conway De Macario E., Macario A. J. L., Wolin M. J. 1982; Antigenic analysis of Methanomicrobiales and Methanobrevibacter arboriphilus. J. Bacteriol. 152:762–764
    [Google Scholar]
  4. Conway De Macario E., Macario A. J. L., Wolin M. J. 1982; Specific antisera and immunological procedures for characterization of methanogenic bacteria. J. Bacteriol. 149:320–328
    [Google Scholar]
  5. Conway De Macario E., Wolin M. J., Macario A. J. L. 1982; Antibody analysis of relationship among methanogenic bacteria. J. Bacteriol. 149:316–319
    [Google Scholar]
  6. Corder R. E., Hook L. A., Larkin J. M., Frea J. I. 1983; Isolation and characterization of two new methane-producing cocci: Methanogenium olentangyi, sp. nov., and Methanococcus deltae, sp. nov. Arch. Microbiol. 134:28–32
    [Google Scholar]
  7. Doddema H. J., Vogels G. D. 1978; Improved identification of methanogenic bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 36:752–754
    [Google Scholar]
  8. Edwards T., McBride B. C. 1975; New method for the isolation and identification of methanogenic bacteria. Appl. Microbiol. 29:540–545
    [Google Scholar]
  9. Ferguson T. J., Mah R. A. 1983; Isolation and characterization of an H2-oxidizing thermophilic methanogen. Appl. Environ. Microbiol. 45:265–274
    [Google Scholar]
  10. Ferry J. G., Smith P. H., Wolfe R. S. 1974; Methanospirillum, a new genus of methanogenic bacteria, and characterization of Methanospirillum hungatii sp. nov. Int. J. Syst. Bacteriol. 24:465–469
    [Google Scholar]
  11. Huber H., Thomm M., Konig H., Thies G., Stetter K. O. 1982; Methanococcus thermolithotrophicus, a novel thermophilic lithotrophic methanogen. Arch. Microbiol. 132:47–50
    [Google Scholar]
  12. Hungate R. E. 1969 A roll tube method for the cultivation of strict anaerobes. 117–132 Norris J. R., Ribbons D. W.ed Methods in microbiology 3B Academic Press, Inc.; New York:
    [Google Scholar]
  13. Jones J. B., Bowers B., Stadtman T. C. 1977; Methanococcus vannielii; ultrastructure and sensitivity to detergents and antibiotics. J. Bacteriol. 130:1357–1363
    [Google Scholar]
  14. Jones W. J., Leigh J. A., Mayer F., Woese C. R., Wolfe R. S. 1983; Methanococcus jannaschii sp. nov., an extremely thermophilic methanogen from a submarine hydrothermal vent. Arch. Microbiol. 136:254–261
    [Google Scholar]
  15. Jones W. J., Paynter M. J. B., Gupta R. 1983; Characterization of Methanococcus maripaludis sp. nov., a new methanogen isolated from salt marsh sediment. Arch. Microbiol. 135:91–97
    [Google Scholar]
  16. Ollivier B. M., Mah R. A., Garcia J. L., Robinson R. 1985; Isolation and characterization of Methanogenium aggregans sp. nov. Int. J. Syst. Bacteriol. 35:127–130
    [Google Scholar]
  17. Paynter M. J. B., Hungate R. E. 1968; Characterization of Methanobacterium mobilis, sp. n., isolated from the bovine rumen. J. Bacteriol. 95:1943–1951
    [Google Scholar]
  18. Preston J. F., Boone D. R. 1973; Analytical determination of the buoyant density of DNA in acrylamide gels after preparative CsCl gradient centrifugation. FEBS Lett. 37:321–324
    [Google Scholar]
  19. Price C. W., Fuson G. B., Phaff H. J. 1978; Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Debaryomyces, and Pichia. Microbiol. Rev. 42:161–193
    [Google Scholar]
  20. Rhodes M. E. 1958; The cytology of Pseudomonas sp. as revealed by a silver-plating method. J. Gen. Microbiol. 18:639–648
    [Google Scholar]
  21. Rivard C. J., Henson J. M., Thomas M. V., Smith P. H. 1983; Isolation and characterization of Methanomicrobium paynteri sp. nov., a mesophilic methanogen isolated from marine sediments. Appl. Environ. Microbiol. 46:484–490
    [Google Scholar]
  22. Rivard C. J., Smith P. H. 1982; Isolation and characterization of a thermophilic marine methanogenic bacterium, Methanogenium thermophilicum sp. nov. Int. J. Syst. Bacteriol. 32:430–436
    [Google Scholar]
  23. Romesser J. A., Wolfe R. S., Mayer F., Spiess E., Walther-Mauruschat A. 1979; Methanogenium, a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp. nov. and Methanogenium marisnigri sp. nov. Arch. Microbiol. 121:147–153
    [Google Scholar]
  24. Stadtman T. C., Barker H. A. 1951; Studies on the methane fermentation. X. A new formate-decomposing bacterium, Methanococcus vannielii. J. Bacteriol. 62:269–280
    [Google Scholar]
  25. Wildgruber G., Thomm M., König H., Ober K., Richiuto T., Stetter K. O. 1982; Methanoplanus limicola, a plate-shaped methanogen representing a novel family, the Methano-planäceae. Arch. Microbiol. 132:31–36
    [Google Scholar]
  26. Zabel H. P., König H., Winter J. 1984; Isolation and characterization of a new coccoid methanogen, Methanogenium tatii sp. nov. from a solfataric field on Mount Tatio. Arch. Microbiol. 137:308–315
    [Google Scholar]
  27. Zinder S. H., Mah R. A. 1979; Isolation and characterization of a thermophilic strain of Methanosarcina unable to use H2-CO2 for methanogenesis. Appl. Environ. Microbiol. 39:996–1008
    [Google Scholar]
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