1887

Abstract

The levels of genetic relatedness of 19 strains which utilize one-carbon compounds were determined by deoxyribonucleic acid (DNA)-DNA hybridization in solution under optimal conditions (S1 nuclease technique). Most of these hyphomicrobia fell into four groups with high levels of relatedness (level of homology within each group, 86 to 110%). These groups were only distantly related to each other (levels of homology between groups, 1 to 9%). Three additional groups of C-utilizing hyphomicrobia were represented by only one strain each. In addition, the levels of DNA-DNA homology of four species and 11 -like isolates were determined. Seven of these isolates formed three groups containing two or three strains each; the level of homology within each group was 94 to 120%. These groups of peptide-utilizing strains were related to spp. at a DNA-DNA homology level of 13 to 43%; thus, they represented new species of Four of the isolates had less than 10% DNA homology with either reference strains or spp. Strain B-1408 was distinguished by its DNA base composition of 46.19 mol% guanine plus cytosine, which is 14 mol% below the average base composition of spp. or spp. The levels of genetic relatedness of other hyphal, budding bacteria, such as spp., genus F, , and genus T, to sp. strain MC-750 were too low to be evaluated by DNA-DNA hybridization techniques.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-2-241
1986-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/2/ijsem-36-2-241.html?itemId=/content/journal/ijsem/10.1099/00207713-36-2-241&mimeType=html&fmt=ahah

References

  1. Aristovskaya T. V. 1961; Accumulation of iron in breakdown of organomineral humus complexes by microorganisms. Dokl. Akad. Nauk SSSR 136:954–957 In Russian
    [Google Scholar]
  2. Aristovskaya T. V. 1963; On the decomposition of organic mineral compounds in podzolic soils. Pochvoved. Akad. Nauk SSSR 1:30–42 In Russian
    [Google Scholar]
  3. Bernardi G. 1969; Chromatography of nucleic acids on hydroxyapatite. I. Chromatography of native DNA. Biochim. Biophys. Acta 174:423–434
    [Google Scholar]
  4. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  5. Duchow E., Douglas H. C. 1949; Rhodomicrobium vannielii, a new photoheterotrophic bacterium. J. Bacteriol. 58:409–416
    [Google Scholar]
  6. Gebers R. 1981; Enrichment, isolation, and emended description of Pedomicrobium ferrugineum Aristovskaya and Pedomicrobium manganicum Aristovskaya. Int. J. Syst. Bacteriol. 31:302–316
    [Google Scholar]
  7. Gebers R., Mandel M., Hirsch P. 1981; Deoxyribonucleic acid base composition and nucleotide distribution of Pedomicrobium spp. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 2:332–338
    [Google Scholar]
  8. Gebers R., Moore R. L., Hirsch P. 1981; DNA-DNA reassociation studies on the genus Pedomicrobium. FEMS Microbiol. Lett. 11:283–286
    [Google Scholar]
  9. Gebers R., Moore R. L., Hirsch P. 1984; Physiological properties and DNA-DNA homologies of Hyphomonas polymorpha and Hyphomonas neptunium. Syst. Appl. Microbiol. 5:510–517
    [Google Scholar]
  10. Gebers R., Wehmeyer U., Roggentin T., Schlesner H., Kolbel-Boelke J., Hirsch P. 1985; Deoxyribonucleic acid base compositions and nucleotide distributions of 65 strains of budding bacteria. Int. J. Syst. Bacteriol. 35:260–269
    [Google Scholar]
  11. Harder W., Matin A., Attwood M. M. 1975; Studies on the physiological significance of the lack of a pyruvate dehydrogenase complex in Hyphomicrobium sp. J. Gen. Microbiol. 86:319–326
    [Google Scholar]
  12. Havenner J. A., McCardell B. A., Weiner R. M. 1979; Development of defined, minimal, and complete media for the growth of Hyphomicrobium neptunium. Appl. Environ. Microbiol. 38:18–23
    [Google Scholar]
  13. Hirsch P. 1968; Biology of budding bacteria. IV. Epicellular deposition of iron by aquatic budding bacteria. Arch. Mikrobiol. 60:201–216
    [Google Scholar]
  14. Hirsch P. 1974; Budding bacteria. Annu. Rev. Microbiol. 28:391–444
    [Google Scholar]
  15. Hirsch P., Conti S. F. 1964; Biology of budding bacteria. I. Enrichment, isolation and morphology of Hyphomicrobium spp. Arch. Mikrobiol. 48:339–357
    [Google Scholar]
  16. Hirsch P., Conti S. F. 1964; Biology of budding bacteria. II. Growth and nutrition of Hyphomicrobium spp. Arch. Mikrobiol. 48:358–367
    [Google Scholar]
  17. Hirsch P., Rheinheimer G. 1968; Biology of budding bacteria. V. Budding bacteria in aquatic habitats: occurrence, enrichment and isolation. Arch. Mikrobiol. 62:289–306
    [Google Scholar]
  18. Kingma-Boltjes T. Y. 1936; Uber Hyphomicrobium vulgare Stutzer et Hartleb. Arch. Mikrobiol. 7:188–205
    [Google Scholar]
  19. Kölbel-Boelke J., Gebers R., Hirsch P. 1985; Genome size determinations for 33 strains of budding bacteria. Int. J. Syst. Bacteriol. 35:270–273
    [Google Scholar]
  20. Lyman J., Fleming R. H. 1940; Composition of seawater. J. Mar. Res. 3:134–146
    [Google Scholar]
  21. Mandel M., Hirsch P., Conti S. F. 1972; Deoxyribonucleic acid base compositions of hyphomicrobia. Arch. Mikrobiol. 81:289–294
    [Google Scholar]
  22. Mandel M., Igambi L., Bergendahl J., Dodsen M. L. Jr., Scheltgen E. 1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J. Bacteriol. 101:333–338
    [Google Scholar]
  23. Mandel M., Leadbetter E. R., Pfenning N., Triiper H. G. 1971; Deoxyribonucleic acid base compositions of phototrophic bacteria. Int. J. Syst. Bacteriol. 21:222–230
    [Google Scholar]
  24. Mevius W. Jr. 1953; Beiträge zur Kenntnis von Hyphomicrobium vulgare Stutzer et Hartleb. Arch. Mikrobiol. 19:1–29
    [Google Scholar]
  25. Moore R. L. 1977; Ribosomal ribonucleic acid cistron homologies among Hyphomicrobium and various other bacteria. Can. J. Microbiol. 23:478–481
    [Google Scholar]
  26. Moore R. L., Hirsch P. 1972; Deoxyribonucleic acid base sequence homologies of some budding and prosthecate bacteria. J. Bacteriol. 110:256–261
    [Google Scholar]
  27. Moore R. L., Hirsch P. 1973; Nuclear apparatus of Hyphomicrobium. J. Bacteriol. 116:1447–1455
    [Google Scholar]
  28. Moore R. L., Weiner R. M., Gebers R. 1984; Genus Hyphomonas Pongratz 1957 nom. rev. emend., Hyphomonas polymorpha Pongratz 1957 nom. rev. emend., and Hyphomonas neptunium (Leifson 1964) comb. nov. emend. (Hyphomicrobium neptunium). Int. J. Syst. Bacteriol. 34:71–73
    [Google Scholar]
  29. Pongratz E. 1957; D’une bactérie pédiculée d’un pus de sinus. Schweiz. Z. Pathol. Bakteriol. 20:593–608
    [Google Scholar]
  30. Powell D. M., Roberson B. S., Weiner R. M. 1980; Serological relationships among budding, prosthecate bacteria. Can. J. Microbiol. 26:209–217
    [Google Scholar]
  31. Schleifer K. H., Stackebrandt E. 1983; Molecular systematics of prokaryotes. Annu. Rev. Microbiol. 37:143–187
    [Google Scholar]
  32. Selin Y. M., Harich B., Johnson J. L. 1983; Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments. Curr. Microbiol. 8:127–132
    [Google Scholar]
  33. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new freshwater prosthecate bacteria. J. Bacteriol. 95:1921–1942
    [Google Scholar]
  34. Steigerwalt A. G., Fanning G. R., Five-Ashbury M. A., Brenner D. J. 1976; DNA relatedness among species of Enterobacter and Serratia. Can. J. Microbiol. 22:121–137
    [Google Scholar]
  35. Stutzer A., Hartleb R. 1898; Untersuchungen über die bei der Bildung von Salpeter beobachteten Mikroorganismen. Mitt. Landwirtsch. Inst. Univ. Breslau 1:75–100
    [Google Scholar]
  36. Weiner R. M., Devine R. A., Powell D. M., Dagasan L., Moore R. L. 1985; Hyphomonas oceanitis sp. nov., Hyphomonas hirschiana sp. nov., and Hyphomonas jannaschiana sp. nov. Int. J. Syst. Bacteriol. 35:237–243
    [Google Scholar]
  37. Zavarzin G. A. 1961; Budding bacteria. Mikrobiologiya 30:774–791 In Russian
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-2-241
Loading
/content/journal/ijsem/10.1099/00207713-36-2-241
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error