1887

Abstract

sp. nov., an obligate anaerobe with desmolytic activity, was isolated from cat feces. Desmolase, which was previously observed only in a species isolated from human fecal flora, cleaves the carbon-carbon bond of 17-hydroxylated corticoids between C-17 and C-20, thereby converting these compounds to androstenes (C steroids). In primary cultures on sheep blood agar plates, forms nonhemolytic, circular to irregular, convex, shiny, entire, white to colorless colonies that are barely visible to the unaided eye. The cells are plump, short rods that are 0.8 to 1.1 μm wide by 1.7 to 2.3 μm long and have capsules and flagella. They occur singly or in short chains and are gram positive. Inositol is the sole carbohydrate fermented. Indole is produced. The major fermentation products in inositol-enriched media are acetic and butyric acids. The type strain is strain ATCC 43058.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-2-183
1986-04-01
2022-08-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/2/ijsem-36-2-183.html?itemId=/content/journal/ijsem/10.1099/00207713-36-2-183&mimeType=html&fmt=ahah

References

  1. Bokkenheuser V. D., Morris G. N., Ritchie A. E., Holdeman L. V., Winter J. 1984; Biosynthesis of androgen from cortisol by a Clostridium species recovered from human fecal flora. J. Infect. Dis. 149:489–494
    [Google Scholar]
  2. Bokkenheuser V. D., Winter J. 1983 Biotransformation of steroids. 215–239 Hentges D. J.ed Human intestinal microflora in health and disease Academic Press, Inc.; New York:
    [Google Scholar]
  3. Bokkenheuser V. D., Winter J., Dehazya P., Kelly W. G. 1977; Isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids. Appl. Environ. Microbiol. 34:571–575
    [Google Scholar]
  4. Eriksson H., Gustafsson J. A. 1971; Excretion of steroid hormones in adults. Eur. J. Biochem. 18:146–150
    [Google Scholar]
  5. Gustafsson J. A. 1968; Steroids in germfree and conventional rats. VII. Identification of C19 and C21 steroids in feces from conventional rats. Eur. J. Biochem. 6:248–255
    [Google Scholar]
  6. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1978 Anaerobe laboratory manual. , 4th ed.. Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  7. Holdeman L. V., Moore W. E. C. 1974 Genus II. Eubacterium Prevot 1938, 294. 641–657 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th ed.. The William & Wilkins Co.; Baltimore:
    [Google Scholar]
  8. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt T.ed Manual of methods for general microbiology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  9. Macdonald I. A., Jellett J. F., Mahoney D. E., Holdeman L. V. 1979; Bile salt 3a- and 12a-hydroxysteroid dehydrogenases from Eubacterium lentum and related organisms. Appl. Environ. Microbiol. 37:992–1000
    [Google Scholar]
  10. Macdonald I. A., Rochon Y. P., Hutchinson D. M., Holdeman L. V. 1982; Formation of ursodeoxycholic acid from chenodeoxycholic acid by 7β-hydroxy steroid dehydrogenaseelaborating Eubacterium aerofaciens strain co-cultured with 7a-hydroxysteroid dehydrogenase-elaborating organism. Appl. Environ. Microbiol. 44:1187–1195
    [Google Scholar]
  11. Marmur G., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  12. Migeon C. J., Sandberg A. A., Samuels L. T. 1956; Metabolism of 4-C14-corticosterone in man. J. Clin. Endocrinol. 16:1291–1298
    [Google Scholar]
  13. Nabarro J. D. N., Moxham A., Walker G., Slater J. D. H. 1957; Rectal hydrocortisone. Br. Med. J. 2:272–274
    [Google Scholar]
  14. Taylor W. 1970; Steroid metabolism in the rabbit. Biliary and urinary excretion of metabolites of (4-14C-)cortisone. Biochem. J. 117:263–265
    [Google Scholar]
  15. Wade A. P., Slater D. M., Kellie A. F., Holliday M. E. 1959; Urinary excretion of 17-keto steroids following rectal infusion of cortisol. J. Clin. Endocrinol. Metab. 19:444–453
    [Google Scholar]
  16. White B. A., Lipsky R. L., Fricke R. J., Hylemon P. B. 1980; Bile acid induction specificity of 7a-dehydroxylase activity in an intestinal Eubacterium species. Steroids 35:103–110
    [Google Scholar]
  17. Wilkins T. D., Thiel T. 1973; Modified broth-disk method for testing the antibiotic susceptibility of anaerobic bacteria. Antimicrob. Agents chemother. 3:350–356
    [Google Scholar]
  18. Winter J., Morris G. N., O’Rourke-Locascio S., Bokkenheuser V. D. 1984; Mode of action of steroid desmolase and reductases synthesized by Clostridium “scindens” (formerly Clostridium strain 19). J. Lipid Res. 25:1124–1131
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-2-183
Loading
/content/journal/ijsem/10.1099/00207713-36-2-183
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error