sp. nov. is a gram-negative, heterotrophic bacterium that was isolated from a culture of which had been grown autotrophically on FeSO-basal salts medium for several years. Purification of was carried out on a 1.0% glucose-basal salts medium (pH 3.0) solidified with agarose. Growth was enhanced by adding high concentrations of glucose (0.5 to 2.0%) and by supplementing the medium with yeast extract and trace amounts of FeSO. However, these supplements were not necessary for growth. A wide variety of organic compounds were suitable substrates for growth, but inorganic forms of reduced sulfur or ferrous iron were not. Doubling times of 2.5 h and cell densities of >2 × 10cells per ml were obtained at the optimal temperature of 37°C and pH 3.0. The guanine-plus-cytosine content of the deoxyribonucleic acid was 64 mol%. contains at least three distinct plasmids; one of these plasmids is larger than 30 kilobase pairs, and two are smaller than 4.0 kilobase pairs. Homology studies in which we compared the total deoxyribonucleic acid of with the total deoxyribonucleic acids of and several species indicated that is most closely related to can be differentiated from by its higher temperature optimum, its faster growth rate, and its inability to utilize reduced forms of sulfur or iron as energy sources. The abundant cell growth that occurs in a medium which either is rich in organic compounds or completely lacks nutritional supplements distinguishes from The other physiological and genetic characteristics which we examined are in close agreement with the characteristics of members of the genus The type strain of is strain ATCC 43141.


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