1887

Abstract

sp. nov. is a gram-negative, heterotrophic bacterium that was isolated from a culture of which had been grown autotrophically on FeSO-basal salts medium for several years. Purification of was carried out on a 1.0% glucose-basal salts medium (pH 3.0) solidified with agarose. Growth was enhanced by adding high concentrations of glucose (0.5 to 2.0%) and by supplementing the medium with yeast extract and trace amounts of FeSO. However, these supplements were not necessary for growth. A wide variety of organic compounds were suitable substrates for growth, but inorganic forms of reduced sulfur or ferrous iron were not. Doubling times of 2.5 h and cell densities of >2 × 10cells per ml were obtained at the optimal temperature of 37°C and pH 3.0. The guanine-plus-cytosine content of the deoxyribonucleic acid was 64 mol%. contains at least three distinct plasmids; one of these plasmids is larger than 30 kilobase pairs, and two are smaller than 4.0 kilobase pairs. Homology studies in which we compared the total deoxyribonucleic acid of with the total deoxyribonucleic acids of and several species indicated that is most closely related to can be differentiated from by its higher temperature optimum, its faster growth rate, and its inability to utilize reduced forms of sulfur or iron as energy sources. The abundant cell growth that occurs in a medium which either is rich in organic compounds or completely lacks nutritional supplements distinguishes from The other physiological and genetic characteristics which we examined are in close agreement with the characteristics of members of the genus The type strain of is strain ATCC 43141.

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1986-04-01
2023-02-09
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References

  1. Arkesteyn G. J. M. W., deBont J. A. M. 1980; Thiobacillus acidophilus·, a study of its presence in Thiobacillus ferrooxidans cultures. Can. J. Microbiol. 26:1057–1065
    [Google Scholar]
  2. Barros M. E. C., Rawlings D. E., Woods D. R. 1984; Mixotrophic growth of a Thiobacillus ferrooxidans strain. Appl. Environ. Microbiol. 47:593–595
    [Google Scholar]
  3. Blin N., Stafford D. W. 1976; Isolation of high molecular-weight DNA. Nucleic Acids Res. 3:2303–2311
    [Google Scholar]
  4. Guay R., Silver M. 1975; Thiobacillus acidophilus sp. nov., isolation and some physiological characteristics. Can. J. Microbiol. 21:281–288
    [Google Scholar]
  5. Harrison A. P. Jr. 1981; Acidiphilium cryptum gen. nov., sp. nov., heterotrophic bacterium from acidic mineral environments. Int. J. Syst. Bacteriol. 31:327–332
    [Google Scholar]
  6. Harrison A. P. Jr. 1984; The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. Annu. Rev. Microbiol. 38:265–292
    [Google Scholar]
  7. Harrison A. P. Jr., Jarvis B. W., Johnson J. L. 1980; Heterotrophic bacteria from cultures of autotrophic Thiobacillus ferrooxidans: relationships as studied by means of deoxyribonucleic acid homology. J. Bacteriol. 143:448–454
    [Google Scholar]
  8. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:193–197
    [Google Scholar]
  9. Ingledew W. J. 1982; Thiobacillus ferrooxidans: the bioenergetics of an acidophilic chemolithotroph. Biochim. Biophys. Acta 683:89–117
    [Google Scholar]
  10. Johnson D. B., Kelso W. I. 1983; Detection of heterotrophic contaminants in cultures of Thiobacillus ferrooxidans and their elimination by subculturing in media containing copper sulfate. J. Gen. Microbiol. 129:2969–2972
    [Google Scholar]
  11. Ko C. Y., Johnson J. L., Barnett L. B., McNair H. M., Vercellotti J. R. 1977; A sensitive estimation of the percentage of guanine plus cytosine in deoxyribonucleic acid by high performance liquid chromatography. Anal. Biochem. 80:183–192
    [Google Scholar]
  12. Manning H. L. 1975; New medium for isolating iron-oxidizing and heterotrophic acidophilic bacteria from acid mine drainage. Appl. Microbiol. 30:1010–1016
    [Google Scholar]
  13. Martin P. A. W., Dugan P. R., Tuovinen O. H. 1981; Plasmid DNA in acidophilic, chemolithotrophic thiobacilli. Can. J. Microbiol. 27:850–853
    [Google Scholar]
  14. Mishra A. K., Roy P. 1979; A note on the growth of Thiobacillus ferrooxidans on solid medium. J. Appl. Bacteriol. 47:289–292
    [Google Scholar]
  15. Mishra A. K., Roy P., Mahapatra S. S. R. 1983; Isolation of Thiobacillus ferrooxidans from various habitats and their growth pattern on solid medium. Curr. Microbiol. 8:147–152
    [Google Scholar]
  16. Norris P. R., Kelly D. P. 1982 The use of mixed microbial cultures in metal recovery. 443–474 Bull A. T., Slater J. H.ed Microbial interactions and communities 1 Academic Press, Inc.; New York:
    [Google Scholar]
  17. Orskov F. 1974 Genus Escherichia. 293–296 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  18. Schnaitman C., Lundgren D. G. 1965; Organic compounds in the spent medium of Ferrobacillus ferrooxidans. Can. J. Microbiol. 11:23–27
    [Google Scholar]
  19. Shafia F., Brinson K. R., Heinzman M. W., Brady J. M. 1972; Transition of chemolithotroph Ferrobacillus ferrooxidans to obligate organotrophy and metabolic capabilities of glucose-grown cells. J. Bacteriol. 111:56–65
    [Google Scholar]
  20. Shafia F., Wilkinson R. F. Jr. 1969; Growth of Ferrobacillus ferrooxidans on organic matter. J. Bacteriol. 97:256–260
    [Google Scholar]
  21. Silverman M. P., Lundgren D. G. 1959; Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J. Bacteriol. 77:624–647
    [Google Scholar]
  22. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517
    [Google Scholar]
  23. Sugio T., Kudo S., Tano T., Imai K. 1982; Glucose transport system in facultative iron-oxidizing bacterium, Thiobacillus ferrooxidans. J. Bacteriol. 150:1109–1114
    [Google Scholar]
  24. Tabita R., Lundgren D. G. 1971; Utilization of glucose and the effect of organic compounds on the chemolithotroph Thiobacillus ferrooxidans. J. Bacteriol. 108:328–333
    [Google Scholar]
  25. Tabita R., Lundgren D. G. 1971; Heterotrophic metabolism of the chemolithotroph Thiobacillus ferrooxidans. J. Bacteriol. 108:334–342
    [Google Scholar]
  26. Tamaoka , Komagata K. 1984; Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  27. Tuovinen O. H., Nicholas D. J. D. 1977; Transition of Thiobacillus ferrooxidans KG-4 from heterotrophic growth on glucose to autotrophic growth on ferrous-iron. Arch. Microbiol. 114:193–195
    [Google Scholar]
  28. Xingshi P., Yecheng W., Yaobo L., Xianwu Z. 1981; Determination of mole percent of guanine + cytosine in bacterial DNA by means of HPLC. Acta Microbiol. Sin. 21:339–343
    [Google Scholar]
  29. Zavarzin G. 1972; A heterotrophic satellite of Thiobacillus ferrooxidans. Mikrobiologiya 41:369–370
    [Google Scholar]
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