1887

Abstract

Natural isolates of from a variety of habitats were compared on the basis of seven isoenzyme patterns. Some strains of considered to be anamorphs, as well as one strain each of , and , were also examined. The isoenzyme patterns readily substantiated the relationships between the anamorphs and their putative teleomorphs and the nonmember status of strains not belonging to the genus. A multivariate analysis of the electrophoretic patterns indicated that isolates belonging to the and deoxyribonucleic acid reassociation groups are not phenotypically continuous with one another or with the former species , and thus we propose to reinstate these taxa as separate species. Taxa previously described as and also appeared to constitute reproductively isolated natural populations. When the results of isoenzyme electrophoresis were compared with deoxyribonucleic acid reassociation data and with mating compatibility patterns determined by other workers, we found that deoxyribonucleic acid relatedness gives a more accurate view of relationships among species.

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1986-01-01
2024-06-15
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References

  1. Chatfield G., Collins A. J. 1980 Introduction to multivariate analysis. Chapman & Hall; New York:
    [Google Scholar]
  2. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121:404–427
    [Google Scholar]
  3. Eilers F. I., Allen J., Hill E. P., Sussman A. S. 1964; Localization of disaccharides in extracts of Neurospora after electrophoresis in polyacrylamide gels. J. Histochem. Cytochem. 12:448–450
    [Google Scholar]
  4. Hill M. O. 1973; Reciprocal averaging, an eigenvector method of ordination. J. Ecol. 61:237–249
    [Google Scholar]
  5. Johannsen E. 1980; Hybridization studies within the genus Kluyveromyces van der Walt emend, van der Walt. Antonie van Leeuwenhoek J. Microbiol. Serol. 46:177–189
    [Google Scholar]
  6. Lachance M. A. 1980 Yeasts associated with black knot disease of trees. 607–613 Stewart G. G., Russell I.ed Current developments in yeast research Pergamon Press; Toronto:
    [Google Scholar]
  7. Martini A. 1973; Ibridazioni DNA/DNA tra specie di lieviti del genere Kluyveromyces. Ann. Fac. Agrar. Univ. Perugia 28:157–171
    [Google Scholar]
  8. Nei M. 1972; Genetic distance between populations. Am. Nat. 106:283–292
    [Google Scholar]
  9. Phaff H. J. 1980 The species concept in yeast: physiologic, morphologic, genetic, and the ecological parameters. 635–643 Stewart G. G., Russell I.ed Current developments in yeast research Pergamon Press; Toronto:
    [Google Scholar]
  10. Schmidt J. L., Graffard C., Lenoir J. 1979; Contribution a l’etude de levures isolees du fromage camembert. I. Essais préliminaires. Lait 59:142–163
    [Google Scholar]
  11. Sidenberg D. G., Lachance M. A. 1982; Multiple molecular forms of exo-β-glucanase in Kluyveromyces species. Exp. Mycol. 6:84–89
    [Google Scholar]
  12. Sidenberg D. G., Lachance M. A. 1983; Speciation, species delineation, and electrophoretic isoenzyme patterns of the type strains of Kluyveromyces van der Walt emend, van der Walt. Int. J. Syst. Bacteriol. 33:822–828
    [Google Scholar]
  13. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy. W. H. Freeman; San Francisco:
    [Google Scholar]
  14. van der Walt J. P. 1970 Kluyveromyces van der Walt emend, van der Walt. 316–378 Lodder J.ed The yeasts, a taxonomic study North-Holland Publishing Co.; Amsterdam:
    [Google Scholar]
  15. van der Walt J. P., Johannsen E. 1984 Kluyveromyces van der Walt emend, van der Walt. 224–251 Kreger-van Rij N. J. W.ed The yeasts, a taxonomic study North-Holland Publishing Co.; Amsterdam:
    [Google Scholar]
  16. van der Walt J. P., Nel E. E. 1963; Saccharomyces vanudenii nov. spec. Mycopathol. Mycol. Appl. 20:71–74
    [Google Scholar]
  17. Yamazaki M., Goto S., Komagata K. 1982; Comparison of enzymes from strains of wine yeasts and their related yeasts on electrophoresis. J. Inst. Enol. Vitic. Yamazaki Univ. 17:11–19
    [Google Scholar]
  18. Yamazaki M., Goto S., Komagata K. 1983; An electrophoretic comparison of the enzymes of Saccharomyces yeasts. J. Gen. Appl. Microbiol. 29:305–318
    [Google Scholar]
  19. Yamazaki M., Komagata K. 1982; Asporogenous yeasts and their supposed ascosporogenous states; an electrophoretic comparison of enzymes. J. Gen. Appl. Microbiol. 28:119–138
    [Google Scholar]
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