1887

Abstract

Ribosomal ribonucleic acid (RNA) homology studies indicated that there is 90 to 96% homology between and and 64 to 70% homology between these two species and . These findings support the inclusion of these three species in the genus . In contrast, “” strains showed very little homology with the other species (< 22% RNA homology) and should not be considered a member of this genus. The taxonomic placement of “” is uncertain. The nearest relatives of the genus were and (>65% RNA homology); and exhibited 30 to 60% RNA homology with species. Deoxyribonucleic acid studies indicated that subsp. was related to at a level of deoxyribonucleic acid homology of >45% and at a level of RNA homology of 99%; moreover, this organism was found to be a microaerophilic nitrogen fixer. Thus, subsp. is a subjective synonym of . In contrast, deoxyribonucleic acid homology studies indicated that subsp. is not related to , or any other species tested, and its taxonomic position is uncertain. Several strains of azospirilla which form unique star-shaped colonies were identified as by deoxyribonucleic acid homology.

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1986-01-01
2024-04-19
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References

  1. Baldani J. L, Baldani V. L. D., Sampaio M. J. A. M., Döbereiner J. 1984; A fourth Azospirillum species from cereal roots. An. Acad. Bras. Cien. 56:365
    [Google Scholar]
  2. Beijerinck M. 1925; Über ein Spirillum, welches freien Stickstoff binden kann? Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 63:353–359
    [Google Scholar]
  3. Caraway B. H., Krieg N. R. 1974; Aerotaxis in Spirillum volutans. Can. J. Microbiol. 20:1367–1377
    [Google Scholar]
  4. De Smedt J., Bauwens M., Tytgat R., De Ley J. 1980; Intra- and intergeneric similarities of ribosomal ribonucleic acid cistrons of free-living, nitrogen-fixing bacteria. Int. J. Syst. Bacteriol. 30:106–122
    [Google Scholar]
  5. Döbereiner J., Day J. M. 1976 Associative symbioses in tropical grasses; characterization of microorganisms and dinitrogen fixing sites. 518–538 Newton W. E., Nymans C. J.ed Symposium on nitrogen fixation Washington State University Press; Pullman:
    [Google Scholar]
  6. Falk E. C., Döbereiner J., Johnson J. L., Krieg N. R. 1985; Deoxyribonucleic acid homology of Azospirillum amazonense Magalhäes et al. 1984 and emendation of the description of the genus Azospirillum. Int. J. Syst. Bacteriol. 35:117–118
    [Google Scholar]
  7. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol. 12:829–842
    [Google Scholar]
  8. Johnson J. L. 1978; Taxonomy of the Bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species. Int. J. Syst. Bacteriol. 28:245–256
    [Google Scholar]
  9. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  10. Krieg N. R., Döbereiner J. 1984 The genus Azospirillum,. 94–104 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  11. Magalhāes F. M., Baldani J. L., Souto S. M., Kuykendall J. R., Döbereiner J. 1983; A new acid-tolerant Azospirillum species. An. Acad. Bras. Cien. 55:417–430
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  13. Micales B. K., Johnson J. L., Claus G. W. 1985; Deoxyribonucleic acid homologies among organisms in the genus Gluconobacter. Iht. J. Syst. Bacteriol. 35:79–85
    [Google Scholar]
  14. Selin Y. M., Harich B., Johnson J. L. 1983; Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments. Curr. Microbiol. 8:127–132
    [Google Scholar]
  15. Skerman V. B. D., Sly L. I., Williamson M. 1983; Conglomeromonas largomobilis gen. nov., sp. nov., a sodium-sensitive, mixed-flagellated organism from freshwaters. Int. J. Syst. Bacteriol. 33:300–308
    [Google Scholar]
  16. Tarrand J. J., Krieg N. R., Döbereiner J. 1978; A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can. J. Microbiol. 24:967–980
    [Google Scholar]
  17. Tereba A., McCarthy B. J. 1973; Hybridization of 125I-labeled ribonucleic acid. Biochemistry 12:4675–4679
    [Google Scholar]
  18. Woese C. R., Blanz P., Hespell R. B., Hahn C. M. 1982; Phylogenetic relationships among various helical bacteria. Curr. Microbiol. 7:119–124
    [Google Scholar]
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