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A total of 22 species of the order Mycoplasmatales, representing eight serologically distinct groups, were tested for the presence of the first two enzymes of the arginine dihydrolase pathway by using two-dimensional thin-layer chromatography to separate end products. Both citrulline, produced by arginine deiminase, and ornithine, produced by ornithine carbamoyltransferase, were found in the glycolytic serogroup 1 species Mycoplasma putrefaciens, Mycoplasma capricolum, and Mycoplasma sp. bovine group 7, in Mycoplasma fermentans (serogroup 6), and in the nonfermentative serogroup 7 species Mycoplasma arginini, Mycoplasma hominis, Mycoplasma gallinarum, Mycoplasma gateae, Mycoplasma salivarium, Mycoplasma orale, and Mycoplasma buccale. Citrulline alone was found in one glycolytic species, Mycoplasma gallisepticum (serogroup 4). No end products were found in six other fermentative Mycoplasma species, in three Acholeplasma species, or in two Ureaplasma urealyticum serovars. The use of radiolabeled substrate with representative species showed that citrulline and ornithine were produced from arginine and not derived from the medium.
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