1887

Abstract

A total of 22 species of the order , representing eight serologically distinct groups, were tested for the presence of the first two enzymes of the arginine dihydrolase pathway by using two-dimensional thin-layer chromatography to separate end products. Both citrulline, produced by arginine deiminase, and ornithine, produced by ornithine carbamoyltransferase, were found in the glycolytic serogroup 1 species , and sp. bovine group 7, in (serogroup 6), and in the nonfermentative serogroup 7 species , and . Citrulline alone was found in one glycolytic species, (serogroup 4). No end products were found in six other fermentative species, in three species, or in two serovars. The use of radiolabeled substrate with representative species showed that citrulline and ornithine were produced from arginine and not derived from the medium.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-1-60
1986-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/1/ijsem-36-1-60.html?itemId=/content/journal/ijsem/10.1099/00207713-36-1-60&mimeType=html&fmt=ahah

References

  1. Abdelel A. T. 1979; Arginine catabolism by microorganisms. Annu. Rev. Microbiol. 33:139–168
    [Google Scholar]
  2. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologie techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  3. Ball H. J., Neill S. D., Reed R. L. 1985; Aminopeptidase activity in arginine-utilizing Mycoplasma spp. J. Clin. Microbiol. 21:859–860
    [Google Scholar]
  4. Barile M. F. 1983 Arginine hydrolysis. 345–349 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  5. Barile M. F., DelGiudice R. A., Carski T. R., Gibbs C. J., Morris J. A. 1968; Isolation and characterization of Mycoplasma arginini spec. nov. Proc. Soc. Exp. Biol. Med. 129:489–494
    [Google Scholar]
  6. Barile M. F., Schimke R. T., Riggs D. B. 1966; Presence of the arginine dihydrolase pathway in Mycoplasma. J. Bacteriol. 91:189–192
    [Google Scholar]
  7. Chen K. C. S., Culbertson N. J., Knapp J. S., Kenny G. E., Holmes K. K. 1982; Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms. J. Clin. Microbiol. 16:909–919
    [Google Scholar]
  8. Chen K. C. S., Holmes K. K. 1981; A rapid method for detection of bacterial amino acid decarboxylases. Anal. Biochem. 111:60–66
    [Google Scholar]
  9. Cole B. C., Golightly L., Ward J. R. 1967; Characterization of mycoplasma strains from cats. J. Bacteriol. 94:1451–1458
    [Google Scholar]
  10. Cottew G. S., Leach R. S. 1969 Mycoplasmas of cattle, sheep and goats. 527–570 Hayflick L.ed The Mycoplasmatales and the l-phase of bacteria AppletonCentury-Crofts; New York:
    [Google Scholar]
  11. Fenske J. D., Kenny G. E. 1976; Role of arginine deiminase in growth of Mycoplasma hominis. J. Bacteriol. 126:501–510
    [Google Scholar]
  12. Kenny G. E. 1967; Heat stability and organic solvent solubility of mycoplasma antigens. Ann. N.Y. Acad. Sci. 143:676–681
    [Google Scholar]
  13. Kenny G. E. 1977 Antigenic analysis of the Mycoplasmatales. 376–382 Hobson H., Holmes K.ed Nongonococcal urethritis and related infections American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  14. Kenny G. E. 1979 Antigenic determinants. 351–348 Barile M. F., Razin S.ed The mycoplasmas 1 Academic Press, Inc.; New York:
    [Google Scholar]
  15. Kenny G. E. 1983 Agar precipitation and immunoelectrophoretic methods for detection of mycoplasmic antigens. 441–456 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  16. Kenny G. E., Grayston J. T. 1965; Eaton pleuropneumonia-like organism (Mycoplasma pneumoniae) complement-fixing antigen: extraction with organic solvents. J. Immunol. 95:19–25
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275
    [Google Scholar]
  18. Olson N. D., Kerr K. M., Campbell A. 1964; Control of infectious synovitis. XIII. The antigen study of three strains. Avian Dis. 8:209–214
    [Google Scholar]
  19. Razin S., Freundt E. A. 1984 The mycoplasmas. 740–793 Kreig N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  20. Schimke R. T., Barile M. F. 1963; Arginine metabolism in pleuropneumonia-like organisms isolated from mammalian cell culture. J. Bacteriol. 86:195–206
    [Google Scholar]
  21. Shepard M. C., Lunsford C. D., Ford D. K., Purcell R. H., Taylor-Robinson D., Razin S., Black F. T. 1974; Ureaplasma urealyticum gen. nov., sp. nov.: proposed nomenclature for the human T (T-strain) mycoplasmas. Int. J. Syst. Bacteriol. 24:160–171
    [Google Scholar]
  22. Sjostrom K. E., Kenny G. E. 1983; Distinctive antigenic specificities of adenosine triphosphatases and reduced nicotinamide adenine dinucleotide dehydrogenases as means for classification of the order Mycoplasmatales. Int. J. Syst. Bacteriol. 33:218–228
    [Google Scholar]
  23. Skerman V. D. B. 1974 A key for the determination of the generic position of organisms listed in the manual. 1098–1146 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  24. Smith P. F. 1960; Amino acid metabolism of PPLO. Ann N.Y. Acad. Sci. 70:543–550
    [Google Scholar]
  25. Tully J. G, Barile M. F., Edward D. G. ff., Theodore T. S., Erno H. 1974; Characterization of some caprine mycoplasmas, with proposals for new species, M. capricolum and M. putrefaciens. J. Gen. Microbiol. 85:102–120
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-1-60
Loading
/content/journal/ijsem/10.1099/00207713-36-1-60
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error