@article{mbs:/content/journal/ijsem/10.1099/00207713-36-1-1, author = "PIECHULLA, K. and MUTTERS, R. and BURBACH, S. and KLUSSMEIER, R. and POHL, S. and MANNHEIM, W.", title = "Deoxyribonucleic Acid Relationships of “Histophilus ovis/Haemophilus somnus,” Haemophilus haemoglobinophilus, and “Actinobacillus seminis”", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1986", volume = "36", number = "1", pages = "1-7", doi = "https://doi.org/10.1099/00207713-36-1-1", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-36-1-1", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three Australian isolates of “Histophilus ovis,” ten strains of “Haemophilus somnus” from North America, Australia, and Europe, and two American strains of “Haemophilus agni” were investigated by the deoxyribonucleic acid (DNA)-DNA hybridization (renaturation) method to determine their genetic interrelationships and their levels of relatedness to recognized members of the family Pasteurellaceae Pohl 1981. Our results confirmed that “Haemophilus somnus,” “Histophilus ovis,” and one of the “Haemophilus agni” strains studied represent one genetically homogeneous species. This species exhibited up to 41% DNA relatedness to Haemophilus haemoglobinophilus, whereas only insignificant levels of relatedness or no measurable DNA binding was observed with the type species of the genera Actinobacillus, Haemophilus, and Pasteurella and with Haemophilus aphrophilus, Haemophilus ducreyi, Haemophilus paragallinarum, Haemophilus parainfluenzae, Haemophilus segnis, Pasteurella avium, Pasteurella ureae, and “Actinobacillus seminis.” On the other hand, one of the “Haemophilus agni” strains studied (Hoerlein strain M650-1343) was included in the species “Actinobacillus seminis” (DNA binding value, 91%). So far, only low levels of genetic relatedness with “Actinobacillus seminis” and currently recognized members of the family Pasteurellaceae have been detected. The problems of the generic affiliation of the “Histophilus ovis” group, Haemophilus haemoglobinophilus, and “Actinobacillus seminis” and the phenotypic differentiation of these organisms are discussed.", }