1887

Abstract

Deoxyribonucleic acid relatedness of five strains of and also four other species of this genus was assessed by slot blot hybridization. A high degree of genetic relatedness was evident among the strains within the species , but a low degree of relatedness was shown between the species , and . Hybridization homology indicated that is a subjective synonym for and should not have separate species status; therefore, the description of is emended accordingly. The strains that grow optimally at higher temperatures (41°C) and produce brown pigments in the presence of some amino acids may be considered varietal and are referred to as biovar bengal. This study demonstrates the usefulness of slot blot hybridization for quickly assessing the relatedness of different taxonomic groups of bacteria.

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1985-10-01
2024-04-13
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References

  1. Aragno M., Schlegel H. G. 1978; Aquaspirillum autotrophicum a new species of hydrogen-oxidizing, facultatively autotrophic bacteria. Int. J. Syst. Bacteriol. 28:112–116
    [Google Scholar]
  2. Athwal R. S., Deo S. S., Imaeda T. 1984; Deoxyribonucleic acid relatedness among Mycobacterium leprae, Mycobacterium lepraernurium and selected bacteria by dot blot and spectro-photometric deoxyribonucleic acid hybridization assays. Int. J. Syst. Bacteriol. 34:371–375
    [Google Scholar]
  3. Beveridge T. J., Murray R. G. E. 1974; The superficial macromolecular arrays on the cell wall of Spirillum putridiconchylium . J. Bacteriol. 119:1019–1038
    [Google Scholar]
  4. Bradbury W. C., Murray R. G. E., Mancini C., Morris V. L. 1985; Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species. J. Clin. Microbiol. 21:24–28
    [Google Scholar]
  5. Buchanan R. E., Gibbons N. E. (ed.). 1974 Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  6. Burton K. 1968; Determination of DNA concentration with diphenylamine. Methods Enzymol 12B:163–166
    [Google Scholar]
  7. Cohen J., Majors J. E., Varmus H. E. 1979; Organization of mouse mammary tumor virus-specific DNA endogenous to Balb/c mice. J. Virol. 32:483–496
    [Google Scholar]
  8. De Smedt J., Bauwens M., Tijtgat R., De Ley J. 1980; Intra- and intergeneric similarities of ribosomal ribonucleic acid cistrons of free-living, nitrogen-fixing bacteria. Int. J. Syst. Bacteriol. 30:106–122
    [Google Scholar]
  9. Hylemon P. B., Wells J. S., Krieg N. R., Jannasch H. W. 1973; The genus Spirillum a taxonomic study. Int. J. Syst. Bacteriol. 23:340–380
    [Google Scholar]
  10. Kafatos F. C., Jones C. W., Efstratiadis A. 1979; Determination of nucleic acid sequence homologies and relative concentrations by a dot blot hybridization procedure. Nucleic Acids Res. 7:1541–1552
    [Google Scholar]
  11. Kist M. L., Murray R. G. E. 1984; Components of the regular surface array of Aquaspirillum serpens MW5 and their assembly in vitro. J. Bacteriol. 157:599–606
    [Google Scholar]
  12. Koval S. F., Murray R. G. E. 1984; The isolation of surface array proteins from bacteria. Can. J. Biochem. Cell. Biol. 62:1181–1189
    [Google Scholar]
  13. Krieg N. R. 1984; The genus Aquaspirillum . 72–90 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  14. Kumar R., Banerjee A. K., Bowdre J. H., McElroy L. J., Krieg N. R. 1974; Isolation, characterization, and taxonomy of Aquaspirillum bengal sp. nov. Int. J. Syst. Bacteriol. 24:453–458
    [Google Scholar]
  15. Maclnnes J. I., Morris V. L., Flintoff W. F., Kozak C. A. 1984; Characterization and chromosomal location of endogenous mouse mammary tumour virus loci in GR, NFS, and DBA mice. Virology 132:12–25
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning—a laboratory manual Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y.:
    [Google Scholar]
  17. Meyers J. A., Sanchez D., Elwell L. P., Falklow S. 1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J. Bacteriol. 127:1529–1537
    [Google Scholar]
  18. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113:237–251
    [Google Scholar]
  19. Skerman V. B. D. 1967 A guide to the identification of the genera of bacteria, 2.126 The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  20. Swanstrom R., Shank P. R. 1978; X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I. Anal. Biochem. 86:184–192
    [Google Scholar]
  21. Woese C. R., Blanz P., Hespell R. B., Hahn C. M. 1982; Phylogenetic relationships among various helical bacteria. Curr. Microbiol. 7:119–124
    [Google Scholar]
  22. Woese C. R., Weisburg W. G., Paster B. J., Hahn C. M., Tanner R. S., Krieg N. R., Koops H. P., Harms H., Stackebrandt E. 1984; The phylogeny of purple bacteria: the beta subdivision. System. Appl. Microbiol. 5:327–336
    [Google Scholar]
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