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Twenty-four species assigned to the genus Saccharomyces sensu stricto were examined for deoxyribonucleic acid relatedness. Results with type strains demonstrated the presence of four distinct species: S. cerevisiae, S. bayanus, S . carlsbergensis, and S . kluyveri. S . carlsbergensis NRRL Y-12693T showed intermediate relatedness between S . cerevisiae and S . bayanus and has a genome size approximately 1.5 times those of the last two species. These data suggest S . carlsbergensis to be a partial amphidiploid which may have arisen from natural hybridization between S. cerevisiae and S . bayanus.
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