1887

Abstract

We describe a new species of nonpigmented, saccharolytic , , which is frequently isolated from human periodontitis lesions. This species produces an enzyme that hydrolyzes heparin. The major product from glucose is succinic acid. strains have membranes typical of gram-negative cells and external surface structures that stain with ruthenium red and glutaraldehyde-osmium. The guanine-plus-cytosine content of the deoxyribonucleic acid is 47 to 49 mol%. Strains of this species have negligible deoxyribonucleic acid homology with other species described previously. Strain HEP (=ATCC 35895) is the type strain. strains are serologically distinct from other species on the basis of cell agglutination and gel diffusion tests.

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1985-10-01
2024-07-13
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References

  1. Haapasalo M., Lounatmaa K., Ranta H., Shah H., Ranta K. 1985; Ultrastructure of Bacteroides capillus, B . buccae, B. pentosaceus, B. oris, B. oralis, B. veroralis, and peutose sugar-fermenting Bacteroides sp. from humans with periapical osteitis: occurrence of external proteinaceous cell wall layer. Int. J. Syst. Bacteriol 35:65–72
    [Google Scholar]
  2. Holbrook W. P., Duerden B. I. 1974; A comparison of some characteristics of reference strains of Bacteroides oralis with Bacteroides melaninogenicus. Arch. Oral Biol 19:1231–1235
    [Google Scholar]
  3. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual, 4th. Anaerobe Laboratory, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  4. Holdeman L. V., Johnson J. L. 1982; Description of Bacteroides loescheii sp. nov. and emendation of description of Bacteroides melaninogenicus (Oliver and Wherrry) Roy and Kelly 1939 and Bacteroides denticela Shah and Collins 1981. Int. J. Syst. Bacteriol 32:399–409
    [Google Scholar]
  5. Holdeman L. V., Kelley R. W., Moore W. E. C. 1984; Anaerobic gram-negative straight, curved and helical rods. Family 1. Bacteroidaceae. 602–662 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  6. Holdeman L. V., Moore W. E. C., Churn P. J., Johnson J. L. 1982; Bacteroides oris and Bacteroides buccae: new species from human periodontitis and other human infections. Int. J. Syst. Bacteriol 32:125–131
    [Google Scholar]
  7. Johnson J. L. 1981; Genetic characterization. 450–472 Gerhardt P. Manual of methods for general microbiology American Society for Microbiology; Washington D. C:
    [Google Scholar]
  8. Johnson J. L., Holdeman L. V. 1985; Bacteroides capillus Kornman and Holt and Bacteroides pentosaceus Shah and Collins, later synonyms of Bacteroides Holdeman et al. Int. J. Syst. Bacteriol 35:114
    [Google Scholar]
  9. Kornman K. S., Holt S. C. 1981; Physiological and ultrastructural characterization of a new Bacteroides species (Bacteroides capillus) isolated from severe localized periodontitis. J. Periodontal Res 16:542–555
    [Google Scholar]
  10. Listgarten M. A., Lai C.-H. 1979; Comparative ultrastructure of Bacteroides melaninogenicus species. J. Periodontal Res 14:332–340
    [Google Scholar]
  11. Loesche W. J., Socransky S. S., Gibbons R. J. 1964; Bacteroides oralis, proposed new species isolated from the oral cavity of man. J. Bacteriol 88:1329–1337
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  13. Moore W. E. C., Hash D. E., Holdeman L. V., Cato E. P. 1980; Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl. Environ. Microbiol 39:900–907
    [Google Scholar]
  14. Nakamura T. 1969; Study on anaerobic heparinase-producing microorganisms in human oral cavity. Juzen Igakkai Zasshi 78:509–530 In Japanese
    [Google Scholar]
  15. Nakamura T., Suginaka Y., Takazoe I. 1976; Heparinase activity in lesion of periodontal diseases. Bull. Tokyo Dent. Coll 17:147–155
    [Google Scholar]
  16. Nakamura T., Suginaka Y., Takazoe I., Okuda K. 1976; Biological activity of endotoxin from oral heparinase-producing Bacteroides. Matsumoto Shigaku 2:12–19 In Japanese
    [Google Scholar]
  17. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–443
    [Google Scholar]
  18. Shah H. N., Collins M. D. 1981; Bacteroides buccalis, sp. nov., Bacteroides denticola, sp. nov., Bacteroides pentosaceus, sp. nov., new species of the genus Bacteroides from the oral cavity. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 2:235–241
    [Google Scholar]
  19. Slots J. 1977; The predominant cultivable microflora of advanced periodontitis. Scand. J. Dent. Res 5:235–241
    [Google Scholar]
  20. Takazoe I., Nakamura T. 1971; Experimental mixed infection by human gingival crevice material. Bull. Tokyo Dent. Coll 12:85–93
    [Google Scholar]
  21. Taniguchi H., Fujimura S., Takeuchi K., Nakamura T. 1983; Purification and characterization of mucopolysac-charidase from an oral strain of Bacteroides sp. Appl. Environ. Microbiol 46:1252–1257
    [Google Scholar]
  22. Tanner A. C. R., Haffer C., Bratthall G. T., Visconti R. A., Socransky S. S. 1979; A study of bacteria associated with advanced periodontitis in man. J. Clin. Periodontal 6:278–307
    [Google Scholar]
  23. Watabe J., Benno Y., Mitsuoka T. 1983; Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp. nov. Int. J. Syst. Bacteriol 33:57–64
    [Google Scholar]
  24. Yamamoto A., Takazoe I. 1976; Efficient fractionation of collagenolytic sample from Bacteroides melaninogenicus. Bull. Tokyo Dent. Coll 17:123–130
    [Google Scholar]
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