1887

Abstract

We developed a method for polyacrylamide gel electrophoresis of whole cells of spp. The major advantage of this method is its ease of operation since it obviates the need for the preparation of bacterial extracts. Whole-cell samples were prepared by incubating washed, packed cells in 6 M urea at 37°C for 24 h. The results of disc gel electrophoresis of these whole-cell samples were compared with the results of electrophoresis of soluble protein extracts of the bacteria run under the same conditions. A large number of bands were obtained with the whole-cell preparations, and these bands were resolved better than bands obtained with the protein extracts. A total of 22 strains of spp. were examined by polyacrylamide gel electrophoresis of urea-treated whole cells. A cluster analysis of the polyacrylamide gel electrophoresis band patterns showed that the strains clustered first according to serotype and then according to species. Overall, two main divisions were identified, one containing , and and one containing and . This method should be a valuable tool in studying the taxonomy of spp.

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1985-10-01
2024-03-29
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References

  1. Biavati B., Scardovi V., Moore W. E. C. 1982; Electro-phoretic patterns of proteins in the genus Bifidobacterium and proposal of four new species. Int. J. Syst. Bacteriol 32:358–373
    [Google Scholar]
  2. Buchanan A. M., Scott J. L., Gerencser M. A., Beaman B. L., Jang S., Biberstein E. L. 1984; Actinomyces hordeovulneris sp. nov., an agent of canine actinomycosis. Int. J. Syst. Bacteriol 34:439–443
    [Google Scholar]
  3. Cato E. P., Moore W. E. C., Nygaard G., Holdeman L. V. 1984; Actinomyces meyeri sp. nov., specific epithet rev. Int. J. Syst. Bacteriol 34:487–89
    [Google Scholar]
  4. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. 1967; A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal. Biochem 20:150–154
    [Google Scholar]
  5. Collins M. D., Jones D. 1982; Reclassification of Coryne-bacterium pyogenes (Glage) in the genus Actinomyces, as Actinomyces pyogenes comb. nov. J. Gen. Microbiol 128:901–903
    [Google Scholar]
  6. Coykendall A. L., Munzenmaier A. J. 1979; Deoxyribonucleic acid hybridization among strains of Actinomyces viscosus and Actinomyces naeslundii . Int. J. Syst. Bacteriol 29:234–240
    [Google Scholar]
  7. Dent V. E., Williams R. A. D. 1984; Actinomyces denticolens Dent and Williams sp. nov.: a new speices from the dental plaque of cattle. J. Appl. Microbiol 56:183–192
    [Google Scholar]
  8. Dent V. E., Williams R. A. D. 1984; Actinomyces howellii, a new species from the dental plaque of dairy cattle. Int. J. Syst. Bacteriol 34:316–320
    [Google Scholar]
  9. Fillery E. D., Bowden G. H., Hardie J. M. 1978; Comparison of strains of bacteria designated Actinomyces viscosus and Actinomyces naeslundii . Caries Res 12:299–312
    [Google Scholar]
  10. Holmberg K., Nord C. E. 1975; Numerical taxonomy and laboratory identification of Actinomyces Archnia and some related bacteria. J. Gen. Microbiol 91:17–44
    [Google Scholar]
  11. Kersters K., DeLey J. 1975; Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J. Gen. Microbiol 87:333–342
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem 193:265–275
    [Google Scholar]
  13. Moore W. E. C., Hash D. E., Holdeman L. V., Cato E. P. 1980; Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl. Environ. Microbiol 39:900–907
    [Google Scholar]
  14. Panyim S., Chalkley R. 1969; High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys 130:337–346
    [Google Scholar]
  15. Russell R. R. B. 1976; Classification of Streptococcus mutans strains by SDS gel electrophoresis. Microbios Lett 2:55–59
    [Google Scholar]
  16. Schofield G. M., Schaal K. P. 1981; A numerical taxonomic study of members of the Actinomycetaceae and related taxa. J. Gen. Microbiol 127:237–259
    [Google Scholar]
  17. Seiter J. A., Jay J. M. 1980; Application of polyacrylamide gel electrophoresis to the characterization and identification of Arthrobacter species. Int. J. Sys. Bacteriol 30:460–465
    [Google Scholar]
  18. Slack J. M. 1974; Genus Actinomyces . 660–667In Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  19. Spencer E. M., King T. P. 1971; Isoelectric heterogeneity of bovine plasma albumin. J. Biol. Chem 246:201–208
    [Google Scholar]
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