1887

Abstract

Trichloroacetic acid extracts of 119 strains of bacteria were examined by thin-layer chromatography for the presence of 2,3-diamino-2,3-dideoxyhexuronic acid. This compound was identified by thin-layer chromatography as material giving a distinctive blue spot with a ninhydrin-collidine reagent and moving to the same position as authentic 2,3-diamino-2,3-dideoxyglucuronic acid. Material of this kind was found in all strains of propionibacteria except Propionibacterium freudenreichii and also in some strains of Clostridium, Actinomyces, and It was not found in representatives of (11 strains), (4 strains), Lactobacillus (11 strains), Staphylococcus (3 strains), Nocardia (3 strains), Streptomyces (1 strain), Ac tinoplanes (1 strain), (1 strain), (2 strains), and (1 strain), but was present in one of the four strains of examined and in one of two strains of It was not present in whole cells or lipopolysaccharides of seven strains of but was found in two of five Pseudomonas aeruginosa strains tested.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-35-4-411
1985-10-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/35/4/ijs-35-4-411.html?itemId=/content/journal/ijsem/10.1099/00207713-35-4-411&mimeType=html&fmt=ahah

References

  1. Brokopp C. D., Farmer J. J. 1979; Typing methods for Pseudomonas aeruginosa . 89–133 Doggett R. Pseudomonas aeruginosa; clinical manifestations of infection and current therapy Academic Press, Inc.; New York:
    [Google Scholar]
  2. Cato E. P., Cummins C. S., Smith L. DS. 1970; Clostridium limosum André in Prévot 1948, 165: amended description and pathogenic characteristics. Int. J. Syst. Bacteriol. 20:305–316
    [Google Scholar]
  3. Cowgill R. W., Pardee A. B. 1957 Experiments in biochemical research techniques John Wiley & Sons, Inc.; New York:
    [Google Scholar]
  4. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing clostridia. J. Gen. Microbiol. 80:433–442
    [Google Scholar]
  5. Cummins C. S., Johnson J. L. 1981; The genus Propionibacterium . 1894–1902 Starr M. P., Stolp H., Truper H. G., Balows A., Schlegel H. G. The prokaryotes Springer-Verlag; Berlin:
    [Google Scholar]
  6. Cummins C. S., White R. H. 1983; Isolation, identification, and synthesis of 2,3-diamino-2,3-dideoxyglucuronic acid: a component of Propionibacterium acnes cell wall polysaccharide. J. Bacteriol. 153:1388–1393
    [Google Scholar]
  7. Dmitriev B. A., Kocharova N. A., Knirel Y. A., Shaskov A. S., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. 1982; Somatic antigens of Pseudomonas aeruginosa: the structure of the polysaccharide chain of Ps aeruginosa 0:6 (Lanyi) polysaccharide. Eur. J. Biochem. 125:229–237
    [Google Scholar]
  8. Habs I. 1957; Untersuchungen über die O-antigene von Pseudomonas aeruginosa . Z. Hyg. Infektionskr. 144:218–228
    [Google Scholar]
  9. Johnson J. L., Cummins C. S. 1972; Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica . J. Bacteriol. 109:1047–1066
    [Google Scholar]
  10. Knirel Y. A., Kocharova N. A., Shashkov A. S., Dmitriev B. A., Kochetkov N. K. 1981; 2,3-Diacetamido-2,3-dideoxy-d-glucuronic acid: a new acidic amino sugar from Pseudomonas aeruginosa type 06 lipopolysaccharide. Carbohydr. Res. 93:C12–C13
    [Google Scholar]
  11. Knirel Y. A., Vinogradov E. V., Shashkov A. S., Dmitriev B. A., Kochetkov N. K. 1982; 2,3-Diacetamido-2,3-dideoxy-d-mannuronic acid and its 2-imidazoline derivative: new acidic amino sugars from Pseudomonas aeruginosa O:3a,d lipopolysaccharide. Carbohydr. Res. 104:C4–C7
    [Google Scholar]
  12. Knirel Y. A., Vinogradov V., Shaskov A. S., Dmitriev B. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. 1983; Somatic antigens of Pseudomonas aeruginosa: the structure of O-specific polysaccharide chains of Ps aeruginosa 0:3a,b and O:3a,d lipopolysaccharides. Eur. J. Biochem. 128:81–90
    [Google Scholar]
  13. Knirel Y. A., Vinogradov V., Shaskov A. S., Dmitriev B. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. 1983; Somatic antigens of Pseudomonas aeruginosa: the structure of O-specific polysaccharide chains of Ps aeruginosa O:3(a),c and O:3a,d,e lipopolysaccharides. Eur. J. Biochem. 134:289–297
    [Google Scholar]
  14. Lanyi B., Bergan T. 1978; Serological characteristics of Ps aeruginosa . 93–168 Bergan T., Norris J. Methods in microbiology 10 Academic Press, Inc.; New York:
    [Google Scholar]
  15. Lüderitz O., Westphal O., Staub A. M., Nikaido H. 1971; Isolation and chemical and immunological characterization of bacterial lipopolysaccharides. 331–350 Weinbaum G., Kadis S., Ajl S. Microbial toxins 4 Academic Press, Inc.; New York:
    [Google Scholar]
  16. Okuda S., Suzuki N. 1983; 2,3-Diamino-2,3-dideoxy-d-furanurono-6,3-lactam from the hydrolysate of Pseudomonas aeruginosa P14 lipopolysaccharide. Biochem. J. 215:597–604
    [Google Scholar]
  17. Wilkinson S. G. 1983; Composition and structure of lipopolysaccharides from Pseudomonas aeruginosa . Rev. Infect. Dis. 5: Suppl 55S941–S949
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-35-4-411
Loading
/content/journal/ijsem/10.1099/00207713-35-4-411
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error