In this work we were primarily concerned with antigens of strains belonging to the group. The following two antisera were produced: anti- E1 (T = type strain) and anti-. Reference patterns for both of these species were established by crossed immunoelectrophoresis, and these patterns yielded 62 and 70 precipitates, respectively. The crossed reactions of E1 and with E2, , and were studied to test both for total antigens and for certain enzymes (esterase, phosphatase, malate dehydrogenase, and glucose-6-phosphate dehydrogenase). Our results showed that is the species which is closest to , demonstrating more than 70% common antigens, whereas none of the species examined was very closely related to . Species-specific antigens, such as the phosphatase of , as well as antigens common to the group, such as malate dehydrogenase, were also demonstrated in crossed immunoelectrophoresis. This may allow immunological identification of each species.


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