
Full text loading...
In this work we were primarily concerned with antigens of Bacteroides strains belonging to the B. fragilis group. The following two antisera were produced: anti-Bacteroides fragilis E1T (T = type strain) and anti-Bacteroides distasonis. Reference patterns for both of these species were established by crossed immunoelectrophoresis, and these patterns yielded 62 and 70 precipitates, respectively. The crossed reactions of B. fragilis E1T and B. distasonis with Bacteroides fragilis E2, Bacteroides thetaiotaomicron, Bacteroides ovatus, and Bacteroides vulgatus were studied to test both for total antigens and for certain enzymes (esterase, phosphatase, malate dehydrogenase, and glucose-6-phosphate dehydrogenase). Our results showed that B. thetaiotaomicron is the species which is closest to B. fragilis, demonstrating more than 70% common antigens, whereas none of the species examined was very closely related to B. distasonis. Species-specific antigens, such as the phosphatase of B. fragilis, as well as antigens common to the group, such as malate dehydrogenase, were also demonstrated in crossed immunoelectrophoresis. This may allow immunological identification of each species.
Article metrics loading...
Full text loading...
References
Data & Media loading...