Cell extracts from six species, six species, and 23-6 (T = type strain) were examined for enzyme activities of pyrimidine deoxyribonucleotide metabolism. All of these organisms had thymidine kinase and thymidine phosphorylase activities, and all lacked deoxycytidine triphosphatase activity. The 13 members of the were separated into three groups by the presence or absence of the following four enzyme activities: (i) the adenosine triphosphate-insensitive deoxyuridine triphosphate-specific hydrolyzing deoxyuridine triphosphatase, (ii) a deoxyuridine monophosphate phosphatase, (iii) deoxycytidine deaminase, and (iv) deoxycytidine monophosphate deaminase. Five of the six species (all species except L1) had all four enzymatic activities. The six species only had the deoxycytidine and deoxycytidine monophosphate deaminase activities. The only two plant isolates studied, L1 and 23-6, lacked all four enzymatic activities.


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