1887

Abstract

Cell extracts from six species, six species, and 23-6 (T = type strain) were examined for enzyme activities of pyrimidine deoxyribonucleotide metabolism. All of these organisms had thymidine kinase and thymidine phosphorylase activities, and all lacked deoxycytidine triphosphatase activity. The 13 members of the were separated into three groups by the presence or absence of the following four enzyme activities: (i) the adenosine triphosphate-insensitive deoxyuridine triphosphate-specific hydrolyzing deoxyuridine triphosphatase, (ii) a deoxyuridine monophosphate phosphatase, (iii) deoxycytidine deaminase, and (iv) deoxycytidine monophosphate deaminase. Five of the six species (all species except L1) had all four enzymatic activities. The six species only had the deoxycytidine and deoxycytidine monophosphate deaminase activities. The only two plant isolates studied, L1 and 23-6, lacked all four enzymatic activities.

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1985-07-01
2024-04-19
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References

  1. Agutter P. S., Cockrill J. B., Lavine J. E., McCaldin B., Sim R. B. 1979; Properties of mammalian nuclear-envelope nucleoside triphosphatase. Biochem. J. 181:647–658
    [Google Scholar]
  2. Beaman K. D., Pollack J. D. 1981; Adenylate energy charge in Acholeplasma laidlawii . J. Bacteriol. 146:1055–1058
    [Google Scholar]
  3. Fishman W. H. 1974; Perspectives on alkaline phosphatase isoenzymes. Am. J. Med. 56:617–650
    [Google Scholar]
  4. Freundt E. A. 1983; Principles of mycoplasma classification and taxonomy. 9–13 Razin S., Tully J. G. Methods in mycoplasmology 1 Mycoplasma characterization Academic Press, Inc.; New York:
    [Google Scholar]
  5. Freundt E. A., Whitcomb R. F., Barile M. F., Razin S., Tully J. G. 1984; Proposal for elevation of the family Acholeplasmataceae to ordinal rank: Acholeplasmatales . Int. J. Syst. Bacteriol. 34:346–349
    [Google Scholar]
  6. Hamet M., Bonissol C., Cartier P., Houllier A. M., Kona P. 1980; Enzymatic activities on purine and pyrimidine metabolism by nine mycoplasma species contaminating cell cultures. Clin. Chim. Acta 103:15–22
    [Google Scholar]
  7. Jinks D. C., Silvius J. R., McElhaney R. N. 1978; Physiological role and membrane lipid modulation of the membrane-bound (Mg2+ Na+)-adenosine triphosphatase activity in Acholeplasma laidlawii . J. Bacteriol. 136:1027–1036
    [Google Scholar]
  8. Kieiley W. W., Kalckar H. M., Bradley L. B. 1956; The hydrolysis of purine and pyrimidine nucleoside triphosphates by myosin. J. Biol. Chem. 219:95–101
    [Google Scholar]
  9. Lewis M., Weissman S. 1965; Properties of a soluble nucleoside triphosphatase activity in mammalian liver. Arch. Biochem. Biophys. 109:490–498
    [Google Scholar]
  10. Neale G. A. M., Mitchell A., Finch L. R. 1983; Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides . J. Bacteriol. 154:17–22
    [Google Scholar]
  11. Neale G. A. M., Mitchell A., Finch L. R. 1983; Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides . J. Bacteriol. 156:1001–1005
    [Google Scholar]
  12. Ne’eman Z., Kahane I., Razin S. 1971; Characterization of the Mycoplasma membrane proteins. II. Solubilization and enzyme activities of Acholeplasma laidlawii membrane proteins. Biochim. Biophys. Acta 249:169–176
    [Google Scholar]
  13. Pollack J. D. 1975; Localization of reduced nicotinamide adenine dinucleotide oxidase activity in Acholeplasma and Mycoplasma . Int. J. Syst. Bacteriol. 25:108–113
    [Google Scholar]
  14. Pollack J. D. 1983; Localization of enzymes in mycoplasmas: preparatory steps. 327–332 Razin S., Tully J. G. Methods in mycoplasmology 1 Mycoplasma characterization Academic Press, Inc.; New York:
    [Google Scholar]
  15. Pollack J. D., Beaman K. D., Robertson J. A. 1984; Synthesis of lipids from acetate is not characteristic of Acholeplasma or Ureaplasma species. Int. J. Syst. Bacteriol. 34:124–126
    [Google Scholar]
  16. Pollack J. D., Razin S., Pollack M. E., Cleverdon R. C. 1965; Fractionation of mycoplasma cells for enzyme localization. Life Sci. 4:973–977
    [Google Scholar]
  17. Williams M. V., Pollack J. D. 1984; Purification and characterization of a dUTPase from Acholeplasma laidlawii B-PG9. J. Bacteriol. 159:278–282
    [Google Scholar]
  18. Williams M. V., Pollack J. D. 1985; Pyrimidine deoxyribonucleotide metabolism in Acholeplasma laidlawii B-PG9. J. Bacteriol. 161:1029–1033
    [Google Scholar]
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