After enrichment in a medium containing seawater and methanol, 42 methylotrophic strains were isolated. All of these strains were gram-negative, strictly aerobic, motile, rod-shaped organisms that required vitamin B. None grew on methane or on complex nutrient media supplemented or not supplemented with NaCI. All but 2 strains grew on methanol, methylamine, and fructose, 17 strains grew on dimethylamine, and 10 strains grew on trimethylamine. Fructose was the only multicarbon compound tested that was used as a growth substrate. All 11 strains tested used the ribulose monophosphate pathway of carbon assimilation. Depending on the strain, methylamine was oxidized either through a methylamine dehydrogenase or through a methylglut-amate dehydrogenase. The mean guanine-plus-cytosine content of 33 strains was 43 mol%. Based on deoxyribonucleic acid-deoxyribonucleic acid hybridization, two related groups were identified among 11 strains examined. We propose a new genus, , with two species, (type species) and . There was no significant deoxyribonucleic acid hybridization between and the terrestrial obligate methanol utilizers tested. The type strains of and are strains ATCC 35842 (= NCMB 2244) and ATCC 33146 (= NCMB 2163), respectively.


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