1887

Abstract

We prepared antisera against purified inducible β-galactosidases from ATCC 35020, ATCC 11842 (T = type strain), and ATCC 15009 and used these antisera to measure the levels of immunological relatedness between the reference enzymes and β-galactosidases from various lactobacilli. Crude extracts from 11 species of lactobacilli, 7 wild-type strains isolated from Tafí cheese (Tucumán, Argentina), and 4 biotypes isolated from the guts of rats were tested against β-galactosidase antisera from . , . , and . . Pairwise comparisons between cross-reacting crude extracts were done by using double-diffusion assays. These comparisons permitted the recognition of antigenic specificity groups based on the β-galactosidases of various nomenspecies of lactobacilli; some of the species gave bands of nonidentity. The results of microcomplement fixation experiments in which we used two of the three antisera supported the results obtained with the double-diffusion assays and provided quantitative estimations of the antigenic relationships within the various β-galactosidases. From the combined results obtained with the antisera, a composite phylogenetic map of the antigenic relationships among the β-galactosidases from lactobacilli was prepared. This map agreed with the maps for the following two other constitutive enzymes: fructose diphosphate aldolase (J. London and K. Kline, Bacteriol. Rev. 37:453–478, 1973) and -(–)-lactic dehydrogenase (F. Gasser and C. Gasser J. Bacteriol. 106:113–125, 1971).

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1985-01-01
2024-03-02
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