1887

Abstract

A new species, , and three new subspecies, subsp. , subsp. , and subsp. , which were isolated from supragingival and subgingival samples from patients with periodontitis and from patients with experimental gingivitis, are described. These organisms are treponemes that ferment carbohydrates and require rumen fluid or shortchain volatile fatty acids for growth. Fermentable carbohydrates are required as an energy source. The major products of fermentation are acetic, lactic, and succinic acids. Trace amounts of formic acid are also produced. The level of deoxyribonucleic acid-deoxyribonucleic acid homology within each subspecies is greater than 80%, whereas the level of homology between subspecies is about 60%. The average guanineplus-cytosine content of the deoxyribonucleic acid is 51 ± 1 mol%. The type strain of is strain ATCC 35536 (= VPI DR56BRIII6); the type strain of subsp. is strain ATCC 35534 (= VPI D2B8); and the type strain subsp. is strain ATCC 35535 (= VPI D46CPE1).

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1984-10-01
2022-08-15
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References

  1. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing clostridia. J. Gen. Microbiol. 67:33–46
    [Google Scholar]
  2. Cwyk W. M., Canole-Parola E. 1977; Treponema succinifaciens sp. nov.: an anaerobic spirochete from the swine intestine. Arch. Microbiol. 122:231–239
    [Google Scholar]
  3. Holdeman L. V., Cato E. P., Moore W. E. C. (ed.). 1977; Anaerobe laboratory manual. , 4. Anaerobe Laboratory Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  4. Johnson J. L. 1980; Genetic characterization. 450–472 Gerhart P. Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  5. Kinyon J. M., Harris D. L. 1979; Treponema innocens a new species of intestinal bacteria and emended description of the type strain of Treponema hyodysenteriae . Int. J. Syst. Bacteriol. 29:102–109
    [Google Scholar]
  6. Leschine S. B., Canale-Parola E. 1980; Rifampin as a selective agent for isolation of oral spirochetes. J. Clin. Microbiol. 12:772–795
    [Google Scholar]
  7. Loe H., Theilade E., Jensen S. B. 1965; Experimental gingivitis in man. J. Periodontal. 36:177–187
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  9. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  10. Moore W. E. C., Holdeman L. V., Cato E. P., Smibert R. M., Burmeister J. A., Ranney R. R. 1983; Bacteriology of moderate (chronic) periodontitis in mature adult humans. Infect. Immun. 42:510–515
    [Google Scholar]
  11. Moore W. E. C., Holdeman L. V., Smibert R. M., Good I. J., Burmeister J. A., Palcanis K. G., Ranney R. R. 1982; Bacteriology of experimental gingivitis in young adult humans. Infect. Immun. 38:651–667
    [Google Scholar]
  12. Moore W. E. C., Holdeman L. V., Smibert R. M., Hash D. E., Burmeister J. A., Ranney R. R. 1982; Bacteriology of severe periodontitis in young adult humans. Infect. Immun. 38:1137–1148
    [Google Scholar]
  13. Paster B. J., Canale-Parola E. 1982; Physiological diversity of rumen spirochetes. Appl. Environ. Microbiol. 43:686–693
    [Google Scholar]
  14. Selin Y. M., Harich B., Johnson J. L. 1983; Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments. Curr. Microbiol. 8:127–132
    [Google Scholar]
  15. Smibert R. M. 1974; Treponema. 175–184 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  16. Smibert R. M., Burmeister J. A. 1983; Treponema pectinovorum sp. nov. isolated from humans with periodontitis. Int. J. Syst. Bacteriol. 33:852–856
    [Google Scholar]
  17. Stanton T. B., Canale-Parola E. 1979; Enumeration and selective isolation of rumen spirochetes. Appl. Environ. Microbiol. 38:965–973
    [Google Scholar]
  18. Stanton T. B., Canale-Parola E. 1980; Treponema bryantii sp. nov., a rumen spirochete that interacts with cellulolytic bacteria. Arch. Microbiol. 127:145–158
    [Google Scholar]
  19. Wojciechowiez M., Ziolecki A. 1979; Pectinolytic enzymes of large rumen treponemes. Appl. Environ. Microbiol. 37:136–142
    [Google Scholar]
  20. Ziolecki A. 1979; Isolation and characterization of large treponemes from the bovine rumen. Appl. Environ. Microbiol. 37:131–135
    [Google Scholar]
  21. Ziolecki A., Wojciechowiez M. 1980; Small pectinolytic spirochetes from the rumen. Appl. Environ. Microbiol. 39:919–922
    [Google Scholar]
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