@article{mbs:/content/journal/ijsem/10.1099/00207713-34-4-451, author = "Seldin, L. and Van Elsas, J. D. and Penido, E. G. C.", title = "Bacillus azotofixans sp. nov., a Nitrogen-Fixing Species from Brazilian Soils and Grass Roots", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1984", volume = "34", number = "4", pages = "451-456", doi = "https://doi.org/10.1099/00207713-34-4-451", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-34-4-451", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A new species, Bacillus azotofixans, is described. This taxon is based upon 16 soil and root-associated strains that exhibit acetylene-reducing ability and nitrogen-fixing Bacillus sp. strain Hino. B. azotofixans is phenotypically similar to Bacillus polymyxa and Bacillus macerans. However, 13 tests (nitrate reduction; production of acid and gas from xylose, arabinose, lactose, ribose, and glycerol; resistance to lysozyme; liquefaction of gelatin; starch hydrolysis; decomposition of casein and pectin; production of dihydroxyacetone; susceptibility to B. polymyxa phages) differentiate it from B. polymyxa, and 12 characteristics (spore position; Voges-Proskauer test; nitrate reduction; production of acid and gas from xylose, arabinose, lactose, ribose, and glycerol; growth at 45°C; hydrolysis of starch; decomposition of pectin; formation of crystalline dextrins) differentiate it from B. macerans. The guanine-plus-cytosine contents of five strains ranged from 47.9 to 52.5 mol%. All strains reduced acetylene much more efficiently than B.polymyxa or B. macerans. In four strains, nitrogen fixation was confirmed by micro-Kjeldahl analysis of acetylene-reducing cultures. Acetylene reduction was not inhibited by nitrate and was not dependent on yeast extract or thiamin plus biotin. The proposed type strain of B. azotofixans is strain P3L-5 (= ATCC 35681).", }