1887

Abstract

The effects of pH, temperature, the presence or absence of glucose and cellobiose, and culture medium on cellulase synthesis or activity or both by 12 strains of were compared. The liquefaction of carboxymethyl cellulose (CMC) gels by eight of the strains was examined. The optimum temperature for hydrolysis of CMC during growth was 40°C, and the optimum pH was 7 to 7.5. The eight strains broke into two distinct groups with a fourfold difference in rates of CMC or filter paper hydrolysis during growth. The presence of either glucose or cellobiose in the culture medium stimulated growth but repressed either the synthesis or the activity of CMC and filter paper cellulases. This effect is the opposite of that reported previously for CMC cellulase. The greatest rate of filter paper hydrolysis occurred in a synthetic medium. The cell-free carboxymethylcellulase of ATCC 482 was partially purified by molecular exclusion chromatography on Bio-Gel P-100 and Sephadex G-75. The partially purified enzyme had a molecular weight of 40,000 and optimum activity at pH 7.0. Cellobiose was a competitive inhibitor of the enzyme activity. The results obtained with the partially purified enzyme indicated that the results obtained by using CMC gels were valid.

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1984-10-01
2024-04-25
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References

  1. Adachi S. 1965; Thin-layer chromatography of carbohydrates in the presence of bisulfite. J. Chromatogr. 17:295–299
    [Google Scholar]
  2. Beguin P., Eisen H. 1978; Purification and partial characterization of three extracellular cellulases from Cellulomonas sp. Eur. J. Biochem. 87:515–531
    [Google Scholar]
  3. Braden A. R., Thayer D. W. 1976; Serological study of Cellulomonas. Int. J. Syst. Bacteriol. 26:123–126
    [Google Scholar]
  4. Chang W. T. H., Thayer D. W. 1977; The cellulase system of a Cytophaga species. Can. J. Microbiol. 23:1285–1292
    [Google Scholar]
  5. Ghose T. K., Das K. 1971; A simplified kinetic approach to cellulose-cellulase system. Adv. Biochem. Eng. 1:55–76
    [Google Scholar]
  6. Han Y. W., Callihan C. D. 1974; Cellulose fermentation: effect of substrate pretreatment on microbial growth. Appl. Microbiol. 27:159–165
    [Google Scholar]
  7. Han Y. W., Srinivasan V. R. 1968; Isolation and characterization of a cellulose-utilizing bacterium. Appl. Microbiol. 16:1140–1145
    [Google Scholar]
  8. Hofsten B. V. 1975; Topological effects in enzymatic and microbial degradation of highly ordered polysaccharides. 281–295In Symposium on Enzymatic HydrolysisAulanko, Finland
    [Google Scholar]
  9. Hulme M. A. 1971; Viscometric determination of carboxymethylcellulase in standard international units. Arch. Biochem. Biophys. 147:49–54
    [Google Scholar]
  10. Keddie R. M. 1974; Cellulomonas. 629–631 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275
    [Google Scholar]
  12. Menzies I. S., Seakins J. W. T. 1969; Sugars. 310–329 Smith I. Chromatography and electrophoretic techniques 1 Chromatography , 3rd. Interscience Publishers; New York:
    [Google Scholar]
  13. Miller G. L., Blum R., Glennon W. E., Burton A. L. 1960; Measurement of carboxymethylcellulase activity. Anal. Biochem. 2:127–132
    [Google Scholar]
  14. Reese E. T., Siu R. G. H., Levinson H. S. 1950; The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis. J. Bacteriol. 59:485–497
    [Google Scholar]
  15. Scheffe H. 1953; A method for judging all contrasts in the analysis of variance. Biometrika 40:87–104
    [Google Scholar]
  16. Skerman V. B. D. 1967 A guide to the identification of the genera of bacteria, 2nd. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  17. Stackebrandt E., Kandler O. 1979; Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribo-nucleic acid homology, and proposal of seven neotype strains. Int. J. Syst. Bacteriol. 29:273–282
    [Google Scholar]
  18. Stewart B. J., Leatherwood J. M. 1976; Derepressed synthesis of cellulase by Cellulomonas. J. Bacteriol. 128:609–615
    [Google Scholar]
  19. Stoppok W., Rapp P., Wagner F. 1982; Formation, location, and regulation of endo-1,4-3-glucanases and β-glucosidases from Cellulomonas uda. Appl. Environ. Microbiol. 44:44–53
    [Google Scholar]
  20. Thayer D. W. 1978; Carboxymethylcellulase produced by facultative bacteria from the hind-gut of the termite Reticulitermes hesperus. J. Gen. Microbiol. 106:13–18
    [Google Scholar]
  21. Thayer D. W. 1978; Cellulolytic and physiological activities of bacteria during production of single-cell protein from wood. AlChE Symp. Ser. 74:126–135
    [Google Scholar]
  22. Thayer D. W. 1979; Woody plants, a renewable fermentation substrate. 307–318 Goodin J. R., Northington D. K. Arid land plant resources. International Center for Arid and Semi-Arid Land Studies Texas Tech University; Lubbock:
    [Google Scholar]
  23. Thayer D. W., David C. A. 1978; Growth of “seeded” cellulolytic enrichment cultures on mesquite wood. Appl. Environ. Microbiol. 36:291–296
    [Google Scholar]
  24. Thayer D. W., Yang S. P., Ou S.-Y. L. 1978; Single-cell protein from rice hulls. Dev. Ind. Microbiol. 19:385–393
    [Google Scholar]
  25. Yamane K., Suzuki H., Nisizawa K. 1970; Purification and properties of extracellular and cell-bound cellulase components of Pseudomonas fluorescens var. cellulosa. J. Biochem. (Tokyo) 67:19–35
    [Google Scholar]
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