Deoxyribonucleic acid (DNA) was extracted from mycobacterial cells by gentle lysis (sodium dodecyl sulfate and pronase treatment) of wall-deficient forms obtained after D-cycloserine and glycine treatment. DNA from CIP 8139(= ATCC 35219) (T = type strain) was H-labeled by nick-translation. DNA relatedness studies (S1 nuclease method) showed that the seven strains studied form a single DNA hybridization group which is less than 25% related to 16 other species of the genus . The guanine-plus-cytosine content of DNA was 68 mol%, and the guanine-plus-cytosine contents of , and DNAs were 64 to 65 mol%.


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