Deoxyribonucleic acid relatedness between or and other selected bacteria was studied by both dot blot and spectrophotometric deoxyribonucleic acid hybridization assays. The results obtained by the two methods were similar, except for the relatedness values between and two corynebacterial strains. Among the mycobacterial species examined, acid-fast organisms isolated from armadillos and a mangabey monkey with leprosy-like disease showed 100% relatedness with grown experimentally in armadillos, suggesting their common origin. In this study we demonstrate the usefulness of the dot blot hybridization technique as a screening method for mycobacterial taxonomy.


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