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Volume 34, Issue 3

Deoxyribonucleic Acid Relatedness Among Major Human Enterococci Free

Abstract

Strains of enterococci obtained from the American Type Culture Collection and the Centers for Disease Control were examined for deoxyribonucleic acid homology by using the S1 nuclease technique. The strains studied segregated into four homology groups. All five strains were included in a single homology group (group III), and both strains were included in a separate homology group (group IV). Of eight “” strains, six fell into one homology group (group I), and two fell into a second homology group (group II). The four homology groups were easily distinguished phenotypically. All eight “” strains were inactive on mannitol, sorbitol, and arabinose. Group I strains clotted litmus milk but did not form acid from sucrose. Group II strains did not clot litmus milk but formed acid from sucrose. These results are consistent with a classification scheme in which “” is a separate species. We propose that the strains included in homology group I be designated sp. nov. and that strain ATCC 19432 be the type strain of this species. The two strains in homology group II may represent a new species, but the data supporting such a proposal are presently insufficient.

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Copyright © 1984 International Union of Microbiological Societies
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1984-07-01
2023-12-07
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References

  1. Bridge P. D., Sneath P. H. A. 1983; Numerical taxonomy of Streptococcus. . J. Gen. Microbiol. 129:565–597
     [Google Scholar]
  2. Deibel R. H., Lake D. E., Niven C. F. Jr. 1963; Physiology of the enterococci as related to their taxonomy. J. Bacteriol. 86:1275–1282
     [Google Scholar]
  3. Facklam R. R. 1972; Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl. Microbiol. 23:1131–1139
     [Google Scholar]
  4. Facklam R. R. 1980; Streptococci and aerococci. 88–110 Lennette E. H., Balow A., Hausler W. J. Jr., Truant J. P. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  5. Farrow J. A. E., Jones D., Phillips B. A., Collins M. D. 1983; Taxonomic studies on some group D streptococci. J. Gen. Microbiol. 129:1423–1432
     [Google Scholar]
  6. Hartman P. A., Reinhold G. W., Saraswat D. S. 1966; Indicator organisms—a review. I. Taxonomy of the fecal streptococci. Int. J. Syst. Bacteriol. 16:197–221
     [Google Scholar]
  7. Johnson J. L. 1981; Genetic characterization. 450–475 Gerhart P. Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  8. Kalina A. P. 1970; The taxonomy and nomenclature of enterococci. Int. J. Syst. Bacteriol. 20:185–189
     [Google Scholar]
  9. Klipper-Balz R., Fisher G., Schleifer K. H. 1982; Nucleic acid hybridization of group D streptococci. Curr. Microbiol. 7:245–250
     [Google Scholar]
  10. Marmur J., Doty P. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:209–218
     [Google Scholar]
  11. Marmur J., Doty P. 1961; Thermal renaturation of DNA. J. Mol. Biol. 3:585–594
     [Google Scholar]
  12. Roop D. R., Mundt J. O., Riggsby W. S. 1974; Deoxyribonucleic acid hybridization studies among some strains of group D and group N streptococci. Int. J. Syst. Bacteriol. 24:330–337
     [Google Scholar]
  13. Setterstrom J. A., Gross A., Stanko R. S. 1979; Comparison of Minitek and conventional methods for the biochemical characterization of oral streptococci. J. Clin. Microbiol. 10:409–414
     [Google Scholar]
  14. Sherman J. M., Wing H. V. 1937; Streptococcus durans. . J. Dairy Sci. 20:165–167
     [Google Scholar]
  15. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
     [Google Scholar]
  16. Vaughan D. H., Riggsby W. S., Mundt J. O. 1977; Deoxyribonucleic acid relatedness of strains of yellow-pigmented, group D streptococci. Int. J. Syst. Bacteriol. 29:204–212
     [Google Scholar]
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Deoxyribonucleic Acid Relatedness Among Major Human Enterococci
Int J Syst Evol Microbiol 34, 327 (1984); https://doi.org/10.1099/00207713-34-3-327
/content/journal/ijsem/10.1099/00207713-34-3-327
/content/journal/ijsem/10.1099/00207713-34-3-327
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