1887

Abstract

Using phenotypic characterization, we found that 17 strains of asaccharolytic pigmented species isolated from soft-tissue infections in cats could be divided into five distinct groups. All of these strains were catalase positive, required vitamin K-hemin for growth, did not grow in bile, and did not exhibit α-glucosidase activity. The organisms in groups A and E (five strains) did not fluoresce under ultraviolet light at 365 nm and were indole positive. Group A organisms hemagglutinated sheep erythrocytes, but group E strains did not. The members of both of these groups produced large amounts of phenylacetic acid (group A average, 497 μg/ml; group E average, 404 μg/ml), and both groups showed trypsin-like activity (detected by using -benzoyl--arginine-2-napthylamide). The guanine-plus-cytosine contents of the deoxyribonucleic acids of group A strains ranged from 48 to 51 mol%; group E strain guanine-plus-cytosine contents ranged from 44 to 47 mol%. The members of groups B, C, and D (12 strains) all fluoresced and were indole positive. Group D strains hemagglutinated sheep erythrocytes, whereas groups B and C strains did not. Trypsin-like activity occurred in groups C and D but not in group B, and all strains produced phenylacetic acid (group B average, 11 μg/ml; group C average, 313 μg/ml; group D average, 999 μg/ml). The group B, C, and D, deoxyribonucleic acids had the following guanine-plus-cytosine contents: group B, 45 to 47 mol%; group C, 45 to 48 mol%; group D, 49 to 50 mol%. All groups of strains differed phenotypically and by isoelectric focusing of bacterial proteins from the asaccharolytic pigmented type strains ATCC 33277 and ATCC 25260; they were different also from the saccharolytic pigmented type strains ATCC 29147, ATCC 25611, and ATCC 33141.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-34-3-300
1984-07-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/34/3/ijs-34-3-300.html?itemId=/content/journal/ijsem/10.1099/00207713-34-3-300&mimeType=html&fmt=ahah

References

  1. Assche P. F. D. 1978; Differentiation of Bacteroides fragilis species by gas chromatographic detection of phenylacetic acid. J. Clin. Microbiol. 8:614–615
    [Google Scholar]
  2. Coykendall A. L., Kaczmarek F. S., Slots J. 1980; Genetic heterogeneity in Bacteroides asaccharolyticus (Holdeman and Moore 1970) Finegold and Barnes 1977 (Approved Lists, 1980) and proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov. Int. J. Syst. Bacteriol. 30:559–564
    [Google Scholar]
  3. Finegold S. M. 1977; Anaerobic bacteria in human disease. Academic Press, Inc.; New York:
    [Google Scholar]
  4. Finegold S. M., Barnes E. M. 1977; Report of the ICBS Taxonomic Subcommittee on Gram-Negative Anaerobic Rods. Int. J. Syst. Bacteriol. 21:388–394
    [Google Scholar]
  5. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobic laboratory manual. , 4. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  6. Johnson J. L., Holdeman L. V. 1983; Bacteroides intermedius comb. nov. and descriptions of Bacteroides corporis sp. nov. and Bacteroides levii sp. nov. Int. J. Syst. Bacteriol. 33:288–290
    [Google Scholar]
  7. Kaczmarek F. S., Coykendall A. L. 1980; Production of phenylacetic acid by strains of Bacteroides asaccharolyticus and Bacteroides gingivalis (sp. nov.). J. Clin. Microbiol. 12:288–290
    [Google Scholar]
  8. Laliberté M., May rand D. 1983; Characterisation of black-pigmented Bacteroides strains isolated from animals. J. Appl. Bacteriol. 55:247–252
    [Google Scholar]
  9. Laughton B. E., Syed S. A., Loesche W. J. 1982; API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J. Clin. Microbiol. 15:97–102
    [Google Scholar]
  10. Laughton B. E., Syed S. A., Loesche W. J. 1982; Rapid identification of Bacteroides gingivalis. . J. Clin. Microbiol. 15:345–346
    [Google Scholar]
  11. Love D. N., Jones R. F., Bailey M. 1979; Isolation and characterisation of bacteria from abscesses in the subcutis of cats. J. Med. Microbiol. 12:207–212
    [Google Scholar]
  12. Love D. N., Jones R. F., Bailey M. 1979; Clostridium villosum sp. nov. from subcutaneous abscesses in cats. Int. J. Syst. Bacteriol. 29:241–248
    [Google Scholar]
  13. Love D. N., Jones R. F., Bailey M. 1981; Characterisation of Bacteroides species isolated from soft tissue infections in cats. J. Appl. Bacteriol. 50:567–575
    [Google Scholar]
  14. Love D. N., Jones R. F., Bailey N., Johnson R. S., Gamble N. 1982; Isolation and characterisation of bacteria from pyothorax (Empyaemia) in cats. Vet. Microbiol. 7:455–461
    [Google Scholar]
  15. Marmur J. 1961; A procedure of the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  17. Shah H. M., Bonnett R., Mateen B., Williams R. A. D. 1979; The porphyrin pigmentation of subspecies of Bacteroides melaninogenicus. . Biochem. J. 180:45–50
    [Google Scholar]
  18. Slots J., Genco R. J. 1979; Direct hemagglutination technique for differentiating Bacteroides asaccharolyticus oral strains from nonoral strains. J. Clin. Microbiol. 10:371–373
    [Google Scholar]
  19. Slots J., Genco R. J. 1980; Bacteroides melaninogenicus subsp. macacae, a new subspecies from monkey periodonto-pathic indigenous microflora. Int. J. Syst. Bacteriol. 30:82–85
    [Google Scholar]
  20. Slots J., Reynolds H. S. 1982; Long-wave UV light fluorescence for identification of black-pigmented Bacteroides spp. J. Clin. Microbiol. 16:1148–1151
    [Google Scholar]
  21. Syed A. S. 1980; Characteristics of Bacteroides asaccharolyticus from dental plaques of beagel dogs. J. Clin. Microbiol. 11:522–526
    [Google Scholar]
  22. Van Steenbergen T. J. M., Vlaanderen C. A., de Graaff J. 1981; Confirmation of Bacteroides gingivalis as a species distinct from Bacteroides asaccharolyticus. . Int. J. Syst. Bacteriol. 31:236–241
    [Google Scholar]
  23. Watabe J., Benno Y., Mitsouka T. 1983; Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp. nov. Int. J. Syst. Bacteriol. 33:57–64
    [Google Scholar]
  24. Wilkins T. D., Thiel T. 1973; Modified broth-disk method for testing the antibiotic susceptibility of anaerobic bacteria. Antimicrob. Agents Chemother. 3:350–356
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-34-3-300
Loading
/content/journal/ijsem/10.1099/00207713-34-3-300
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error