RT Journal Article SR Electronic(1) A1 RASTOGI, NALIN A1 FREHEL, CLAUDE A1 DAVID, HUGO L.YR 1984 T1 Evidence for Taxonomic Utility of Periodic Acid-Thiocarbohydrazide-Silver Proteinate Cytochemical Staining for Electron Microscopy JF International Journal of Systematic and Evolutionary Microbiology, VO 34 IS 3 SP 293 OP 299 DO https://doi.org/10.1099/00207713-34-3-293 PB Microbiology Society, SN 1466-5034, AB Various species of bacteria belonging to taxonomically separate genera were studied by using electron microscopy and different cytochemical staining methods. Judging from our results and those reported previously in the literature, we concluded that the periodic acid-thiocarbohydrazide-silver proteinate reaction (Thiery method) separates the bacteria into four large groups according to the production of silver grains at the site of the cell wall or the cytoplasmic membrane or both. In the Corynebacterium-Mycobacterium-Nocardia group only the cytoplasmic membrane reacts; in Bacillus and Brucella only the cell wall reacts; in Lactobacillus and Microbacterium both the cell wall and the cytoplasmic membrane react; and in Escherichia and Clostridium neither the cell wall nor the cytoplasmic membrane reacts. Moreover, this staining method clearly separated Mycobacterium leprae from certain leprosy-derived coryneform bacteria., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-34-3-293