1887

Abstract

A new mesophilic, cellulolytic species of was isolated from a methanogenic cellulose enrichment culture of municipal sewage sludge and is described. This species ferments only cellulose and cellobiose. The fermentation products are acetic acid, CO, H, ethanol, and small amounts of lactic acid. The deoxyribonucleic acid base composition is 43 mol% guanine plus cytosine. The name is proposed. Type strain WM2 is deposited in the National Research Council of Canada culture collection as strain NRCC 2944.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-34-2-185
1984-04-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/34/2/ijs-34-2-185.html?itemId=/content/journal/ijsem/10.1099/00207713-34-2-185&mimeType=html&fmt=ahah

References

  1. Ackman R. G. 1972; Porous polymer bead packings and formic acid vapor in GLC of volatile fatty acids. J. Chromatogr. Sci. 10:560–565
    [Google Scholar]
  2. Chen M., Wolin M. J. 1981; Influence of heme and vitamin B12 on growth and fermentations of Bacteroides species.. J. Bacteriol. 145:466–471
    [Google Scholar]
  3. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. , 4. Anaerobe Laboratory, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  4. Holdeman L. V., Moore W. E. C. 1974; Gram-negative anaerobic bacteria,. 384–426 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  5. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bacteriol. Rev. 14:1–49
    [Google Scholar]
  6. Khan A. W., Murray W. D. 1982; Isolation of a symbiotic culture of two species of bacteria capable of converting cellulose to ethanol and acetic acid.. FEMS Microbiol. Lett. 13:377–381
    [Google Scholar]
  7. Khan A. W., Trottier T. M., Patel G. B., Martin S. M. 1979; Nutrient requirement for the degradation of cellulose to methane by a mixed population of anaerobes.. J. Gen. Microbiol. 112:365–372
    [Google Scholar]
  8. Koransky J. R., Allen S. D., Dowell V. R. 1978; Use of ethanol for selective isolation of sporeforming microorganisms. Appl. Environ. Microbiol. 35:762–765
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  10. May field C. I., Inniss W. E. 1977; A rapid, simple method for staining bacterial flagella.. Can. J. Microbiol. 23:1311–1313
    [Google Scholar]
  11. Miller T. L., Wolin M. J. 1974; A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl. Microbiol. 27:985–987
    [Google Scholar]
  12. Miyoshi H. 1978; Characterization of anaerobic cellulolytic bacteria isolated from marine environments. Bull. Jpn. Soc. Sci. Fish. 44:197–202
    [Google Scholar]
  13. Murray W. D., Khan A. W., van den Berg L. 1982; Clostridium saccharolyticurn sp. nov., a saccharolytic species from sewage sludge.. Int. J. Syst. Bacteriol. 32:132–135
    [Google Scholar]
  14. Patel G. B., Khan A. W., Agnew B. J., Colvin J. R. 1980; Isolation and characterization of an anaerobic, cellulolytic microorganism, Acetivibrio cellulolyticus gen. nov., sp. nov.. Int. J. Syst. Bacteriol. 30:179–185
    [Google Scholar]
  15. Ulitzer S. 1972; Rapid determination of DNA base composition by ultraviolet spectroscopy. Biochim. Biophys. Acta 272:1–11
    [Google Scholar]
  16. van Huyssteen J. J. 1967; Gas chromatographic separation of anaerobic digester gases using porous polymer. Water Res. 1:237–242
    [Google Scholar]
  17. Varel V. H., Bryant M. P. 1974; Nutritional features of Bacteroides fragilis subsp. fragilis. . Appl. Microbiol. 18:251–257
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-34-2-185
Loading
/content/journal/ijsem/10.1099/00207713-34-2-185
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error