1887

Abstract

A total of 22 strains of curved, anaerobic bacteria from the vaginae of women with bacterial vaginosis were found to be morphologically, biochemically, and genetically distinct from species in previously described genera. We placed these organisms in the genus gen. nov., which includes curved, anaerobic, motile, rod-shaped bacteria that have multiple subpolar flagella and multilayered gram-variable cell walls, produce succinate and acetate, are stimulated by rabbit serum, and have guanine-plus-cytosine contents of 49 to 52 mol%. is the type species (type strain, ATCC 35241). strains are small (length, 1.7 μm) and gram variable, are stimulated by arginine, and produce ornithine, citrulline, and ammonia from arginine. Six of these strains migrate through soft (0.25%) agar and are nitrate negative, and these organisms are designated subsp. Six strains that do not migrate through soft agar and are nitrate positive are designated subsp. (type strain, ATCC 35242). A total of 10 strains are designated (type strain, ATCC 35243). These organisms are large (length, 2.9 μm) and gram negative, produce acid (pH <5.5) from glycogen, and are CAMP test positive. The levels of deoxyribonucleic acid homology were >75% within and ≤25% between strains of the two species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-34-2-177
1984-04-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/34/2/ijs-34-2-177.html?itemId=/content/journal/ijsem/10.1099/00207713-34-2-177&mimeType=html&fmt=ahah

References

  1. Amsel R., Totten P. A., Spiegel C. A., Chen K. C. S., Eschenbach D., Holmes K. K. 1983; Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am. J. Med. 74:14–22
    [Google Scholar]
  2. Ball H. J., Neill S. D., Reid L. R. 1982; Use of arginine aminopeptidase activity in characterization of arginine-utilizing mycoplasmas. J. Clin. Microbiol. 15:28–34
    [Google Scholar]
  3. Chen K. C. S., Culbertson N. J., Knapp J. S., Kenny G. E., Holmes K. K. 1982; Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms. J. Clin. Microbiol. 16:909–919
    [Google Scholar]
  4. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes.. J. Bacteriol. 115:904–911
    [Google Scholar]
  5. Crow V. L., Thomas T. D. 1982; Arginine metabolism in lactic streptococci.. J. Bacteriol. 150:1024–1032
    [Google Scholar]
  6. Curtis A. H. 1913; A motile curved anaerobic bacillus in uterine discharges.. J. Infect. Dis. 12:165–169
    [Google Scholar]
  7. Durieux R., Dublanchet A. 1980; Les “vibrions” anaérobies des leucorrhées. I. Technique d’isolement et sensibilité aux antibiotiques. Med. Mai. Infect. 10:109–115
    [Google Scholar]
  8. Facklam R. R. 1980; Streptococci and aerococci,. 88–110 Lennette E. H. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  9. Gardner H. L., Dukes C. D. 1955; Hemophilus vaginalis vaginitis. Am. J. Obstet. Gynecol. 69:962–976
    [Google Scholar]
  10. Greenwood J. R., Pickett M. J. 1980; Transfer of Haemophilus vaginalis Gardner and Dukes to a new genus, Gardnerella: G. vaginalis (Gardner and Dukes) comb. nov.. Int. J. Syst. Bacteriol. 30:170–178
    [Google Scholar]
  11. Hjelm E., Hallén A., Forsutn U., Wallin J. 1981; Anaerobic curved rods in vaginitis. Lancet ii:1353–1354
    [Google Scholar]
  12. Hjelm E., Hallén A., Forsum U., Wallin J. 1982; Motile anaerobic curved rods in non-specific vaginitis.. Eur. J. Sex. Trans. Dis. 1:9–14
    [Google Scholar]
  13. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. , 4. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  14. Holst E., Skarin A., Mårdh P.-A. 1982; Characteristics of anaerobic comma-shaped bacteria recovered from the female genital tract.. Eur. J. Clin. Microbiol. 1:310–316
    [Google Scholar]
  15. Johnson J. L. 1981; Genetic characterization,. 457–458 Gerhardt P. Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  16. Kodaka H., Armfleld A. Y., Lombard G. L., Dowell V. R. 1982; Practical procedure for demonstrating bacterial flagella. J.Clin. Microbiol. 16:948–952
    [Google Scholar]
  17. Kodaka H., Lombard G. L., Dowell V. R. 1982; Gas-liquid chromatography technique for detection of hippurate hydrolysis and conversion of fumarate to succinate by microorganisms. J. Clin. Microbiol. 16:962–964
    [Google Scholar]
  18. Koransky J. R., Allen S. D., Dowell V. R. 1978; Use of ethanol for selective isolation of sporeforming organisms. Appl. Environ. Microbiol. 35:762–765
    [Google Scholar]
  19. Krönig I. 1895; Über die Natur der Scheidenheme, specielle über das vorkommen anaërober Streptokokken in Scheidensekret Schwangerer. Zentralbl. Gynaecol. 19:409–412
    [Google Scholar]
  20. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequence of the right operator of phage λ. Proc. Natl. Acad. SciU.S.A. 72:1184–1188
    [Google Scholar]
  21. Mclnerney M. J., Bryant M. P., Hespell R. B., Costerton J. W. 1981; Syntrophomonas wolfei gen. nov., sp. nov., an anaerobic, syntrophilic, fatty acid-oxidizing bacterium. Appl. Environ. Microbiol. 41:1029–1039
    [Google Scholar]
  22. Moore B. 1954; Observations on a group of anaerobic vaginal vibrios. J. Pathol. Bacteriol. 67:461–473
    [Google Scholar]
  23. Peloux Y., Thomas P. 1981; A propos de quelques bacteries mobiles anaerobies gram negatives. Rev. Inst. Pasteur Lyon 14:103–111
    [Google Scholar]
  24. Phillips I., Taylor E. 1982; Anaerobic curved rods in vaginitis. Lancet i:221
    [Google Scholar]
  25. Popp W. 1977; The diagnosis and treatment of mixed anaerobic vaginal discharges. Geburtshilfe Frauenheilkd. 37:432–437
    [Google Scholar]
  26. Prévot A. R. 1940; Manuel de classification et de détermination des bactéries anaérobies. Masson et Cie; Paris:
    [Google Scholar]
  27. Shively J. M. 1974; Inclusion bodies of prokaryotes. Annu. Rev. Microbiol. 28:167–187
    [Google Scholar]
  28. Skarin A., Larsson L., Holst E., Mårdh P.-A. 1982; Gas chromatographic study of cellular fatty acids of comma-shaped bacteria isolated from the vagina.. Eur. J. Clin. Microbiol. 1:307–309
    [Google Scholar]
  29. Skarin A., Mårdh P.-A. 1982; Comma-shaped bacteria associated with vaginitis. Lancet i:342–343
    [Google Scholar]
  30. Sleytr V. B., Glauert A. M. 1976; Ultrastructure of the cell walls of two closely related clostridia that possess different regular arrays of surface subunits.. J. Bacteriol. 126:869–882
    [Google Scholar]
  31. So M., Crosa J. H., Falkow S. 1975; Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids.. J. Bacteriol. 121:234–238
    [Google Scholar]
  32. Spiegel C. A., Amsel R., Eschenbach D., Schoenknecht F., Holmes K. K. 1980; Anaerobic bacteria in nonspecific vaginitis. N. Engl. J. Med. 303:601–607
    [Google Scholar]
  33. Spiegel C. A., Amsel R., Holmes K. K. 1983; Diagnosis of bacterial vaginosis by direct Gram stain of vaginal fluid. J. Clin. Microbiol. 18:170–177
    [Google Scholar]
  34. Spiegel C. A., Eschenbach D. A., Amsel R., Holmes K. K. 1983; Curved anaerobic bacteria in bacterial (nonspecific) vaginosis and their response to antimicrobial therapy. J. Infect. Dis 148:817–822
    [Google Scholar]
  35. Sprott M. S., Ingham H. R., Pattman R. S., Eisenstadt R. L., Short G. R., Narang H. K., Sisson P. R., Selkon J. B. 1983; Characteristics of motile curved rods in vaginal secretions. J. Med. Microbiol. 16:175–182
    [Google Scholar]
  36. Totten P. A., Amsel R., Hale J., Piot P., Holmes K. K. 1982; Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis. . J. Clin. Microbiol. 15:141–147
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-34-2-177
Loading
/content/journal/ijsem/10.1099/00207713-34-2-177
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error