%0 Journal Article %A Coomaraswamy, Umarony %A Gumpf, D. J. %T Immuno-Double-Diffusion Serological Relationships Among Spiroplasma Isolates %D 1984 %J International Journal of Systematic and Evolutionary Microbiology, %V 34 %N 2 %P 173-176 %@ 1466-5034 %R https://doi.org/10.1099/00207713-34-2-173 %I Microbiology Society, %X Spiroplasma citri strains Maroc R8A2T (T = type strain), C189, and C3B, corn stunt Spiroplasma sp. strain E275, and honeybee Spiroplasma sp. strain BC3 all cross-reacted in reciprocal serological tests when an agar double-diffusion system was used. These strains, which are members of Spiroplasma group I, did not show any cross-reactions with flower Spiroplasma strains BNR1 and PPS1, (Spiroplasma floricola and “Spiroplasma apis”) or with the tick-derived organism Spiroplasma mirum. Tick Spiroplasma sp. strain 277F and honeybee Spiroplasma sp. strain AS576 reacted only with antisera prepared against other members of group I. Group III flower spiroplasmas S. floricola 23-6T and BNR1 and group IV strains PPS1 and SR3 did not react with antisera prepared against isolates outside their own groups. The members of each major group showed close antigenic relatedness to each other but not to S. mirum strain SMCAT, which showed minimal one-way cross-reactions with members of group I. Previous serogrouping results for the Spiroplasma strains tested were confirmed by the serological reactions of the organisms in the double-diffusion system. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-34-2-173