1887

Abstract

The deoxyribonucleic acids of two porcine mycoplasmas, and were distinguished readily with restriction enzymes. Enzymes that recognized guanine-plus-cytosine-rich sequences, such as I and HI, were particularly useful since they generated relatively small numbers of deoxyribonucleic acid fragments which could be resolved by gel electrophoresis. In addition, we found that the adenine nucleotide in the GATC sequence of is methylated, whereas that in is not.

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1984-01-01
2022-05-20
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References

  1. Bingham A. H. A., Atkinson T., Scjaky D., Roberts R. J. 1978; A specific endonuclease from Bacillus caldolyticus . Nucleic Acids Res 5:3457–3467
    [Google Scholar]
  2. Boliver F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  3. Bové J. M., Saillard C. 1979; Cell biology of spiroplasmas. 83–153 Whitcomb R. F., Tully J. G. The mycoplasmas 3 Academic Press, Inc.; New York:
    [Google Scholar]
  4. Cerone-McLernon A. M., Furness G. 1980; The preparation of transforming DNA from Mycoplasma hominis strain Sprott tet r and quantitative studies of the factors affecting the genetic transformation of Mycoplasma salivarium strain S9 tets to tetracycline resistance. Can. J. Microbiol 26:1147–1152
    [Google Scholar]
  5. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun 23:641–646
    [Google Scholar]
  6. Dreiseikelmann B., Eichenlaub R., Wackernagel W. 1979; The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 andXDNA on the cleavage by restriction endonuclease Mbo I from Moraxella bovis . Biochim. Biophys. Acta 562:418–428
    [Google Scholar]
  7. Duhorlein V., Linn S., Arber W. 1969; Host specificity of DNA produced by Escherichia coli. XI. In vitro modification of phage fd replicative form. Proc. Natl. Acad. SciU.S.A. 63:556–562
    [Google Scholar]
  8. Dybrig K., Swinton D., Maniloff J., Hattman S. 1982; Cytosine methylation of the sequence GATC in a mycoplasma. J. Bacteriol 151:1420–1424
    [Google Scholar]
  9. Friis N. F. 1975; Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare: a survey. Nord. Veterinaermed 27:337–339
    [Google Scholar]
  10. Geier G. E., Modrich P. 1979; Recognition sequence of the dam methylase of Escherichia coli K-12 and mode of cleavage of Dpn I endonuclease. J. Biol. Chem 254:1408–1413
    [Google Scholar]
  11. Glickman B. W., Vandel Elsen P., Radman M. 1978; Induced mutagenesis in dam mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance. Mol. Gen. Genet 163:307–312
    [Google Scholar]
  12. Israel M. A., Vanderryn D. F., Meltzer D. F., Martin M. A. 1980; Characterization of polyoma viral DNA sequences in polyoma induced hamster tumor cell lines. J. Biol. Chem 255:3798–3805
    [Google Scholar]
  13. Jeffreys A. J., Flavell R. A. 1977; A physical map of the DNA regions flanking the rabbit B-globin gene. Cell 12:429–439
    [Google Scholar]
  14. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. J. Mol. Biol 53:159–162
    [Google Scholar]
  15. Marinus M. G. 1973; Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol. Gen. Genet 127:47–55
    [Google Scholar]
  16. McConnell D., Searcy D., Sutcliffe G. 1978; A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum . Nucleic Acids Res 5:1729–1739
    [Google Scholar]
  17. Morowitz H. J., Wallace D. C. 1973; Genome size and life cycle of the mycoplasma. Ann. N.Y. Acad. Sci 225:62–73
    [Google Scholar]
  18. Razin A. 1978; Methylated bases in the single-stranded DNA phages. 165–175 Denhardt D. T., Dressier D., Ray D. S. The single-stranded DNA phages. Cold Spring Harbor Monograph Series Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  19. Razin A., Razin S. 1980; Methylated bases in mycoplasmal DNA. Nucleic Acids Res 8:1383–1390
    [Google Scholar]
  20. Razin S., Harasawa R., Barile M. F. 1983; Cleavage patterns of the mycoplasma chromosome, obtained by using restriction endonucleases, as indicators of genetic relatedness among strains. Int. J. Syst. Bacteriol 33:201–206
    [Google Scholar]
  21. Razin S., Tully J. G., Rose D. L., Barile M. F. 1983; DNA cleavage patterns as indicators of genotypic heterogeneity among strains of Acholeplasma and Mycoplasma species. J. Gen. Microbiol 129:1935–1944
    [Google Scholar]
  22. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol 113:237–251
    [Google Scholar]
  23. Roberts R. J. 1980; Restriction and modification enzymes and their recognition sequences. Gene 8:329–343
    [Google Scholar]
  24. Roberts R. J. 1981; Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res 9:Y75–Y96
    [Google Scholar]
  25. Rose D. L., Tully J. G., Wittier R. G. 1979; Taxonomy of some swine mycoplasmas: Mycoplasma suipneumoniae Goodwin et al. 1965, a later, objective synonym of Mycoplasma hyopneumoniae Mare and Switzer 1965, and the status of Mycoplasma flocculare Meyling and Friis 1972. Int. J. Syst. Bacteriol 29:83–91
    [Google Scholar]
  26. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol 98:503–517
    [Google Scholar]
  27. Whittlestone P. 1979; Porcine mycoplasmas. 133–176 Tully J. G., Whitcomb R. F. The mycoplasmas 2 Academic Press, Inc.; New York:
    [Google Scholar]
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