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Volume 33, Issue 4

Differentiation of Species by Their Cellular Fatty Acid Composition Free

Abstract

The cellular fatty acid compositions of 10 species, two species, three species, , and Were determined by using capillary gas-liquid chromatography (GLC). The major fatty acids in all species and were hexadecenoic, hexadecanoic, and octadecenoic acids. Qualitative and quantitative differences in hydroxy, branched, and cyclopropane fatty acids and in isomers of unsaturated 16- and 18-carbon acids were used to divide the 29 strains belonging to 17 species tested into 13 GLC groups. Of the 13 groups, 10 contained one species, 2 contained two species, and 1 contained three species. All of the cultures were differentiated from (GLC group I) because the concentration of hexadecenoic acid was greater than the concentration of hexadecanoic acid; in , this ratio was reversed. (GLC group II) and (GLC group III) were differentiated from the and species and from because the cultures did not contain 3-hydroxylauric acid. Seven of 10 species, including (GLC group IV), (GLC group V), (GLC group V), (GLC group V), (GLC group VI), (GLC group VII), and (GLC group VIII), contained both -9-hexadecenoic and -11-hexadecenoic acids. These seven species could be differentiated from (GLC group IX), (GLC group XII), (GLC group XIII), (GLC group XIII), (GLC group XI), (GLC group XI), and (GLC group X) because these latter seven species did not contain -11-hexadecnoic acid. The only cultures which contained cyclopropane acids were and one of the two strains of examined. Branched-chain acids were found in all species tested, and their concentrations ranged from less than 1 to 22%. Although the 16 species tested had many similarities in their cellular fatty acid compositions, there were differences which could be used for differentiation of members of this family at the genus and species levels.

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Copyright © 1983 International Union of Microbiological Societies
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1983-10-01
2024-04-25
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References

  1. Baumann L., Bang S. S., Baumann P. 1980; Study of relationship among species of Vibrio, Photobacterium, and terrestial Enterobacteriaceae by an immunological comparison of glutamine synthetase and superoxide dismutase. Curr. Microbiol. 4:133–138
     [Google Scholar]
  2. Baumann P., Baumann L. 1977; Biology of the marine Enterobacteriaceae: genera Beneckea and Photobacterium . Annu. Rev. Microbiol. 31:39–61
     [Google Scholar]
  3. Baumann P., Baumann L., Bang S. S., Woolkalis M. J. 1980; Reevaluation of the taxonomy of Vibrio, Beneckea, and Photobacterium: abolition of the genus Beneckea . Curr. Microbiol. 4:127–132
     [Google Scholar]
  4. Baumann P., Baumann L., Reichelt J. L. 1973; Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica . J. Bacteriol. 113:1144–1155
     [Google Scholar]
  5. Bøe B., Gjerde J. 1980; Fatty acid patterns in the classification of some representatives of the families Enterobacteriaceae and Vibrionaceae . J. Gen. Microbiol. 116:41–49
     [Google Scholar]
  6. Brian B. L., Gardner E. W. 1968; Fatty acids from Vibrio cholerae lipids. J. Infect. Dis. 118:47–53
     [Google Scholar]
  7. Broady K. W., Rietschel E. T., Luderitz O. 1981; The chemical structure of the lipid A component of lipopolysaccharides from Vibrio cholerae . Eur. J. Biochem. 115:463–468
     [Google Scholar]
  8. Campbell I. M., Naworal J. 1969; Mass spectral discrimination between monoenoic and cyclopropanoid, and between normal, iso, and anteiso fatty acid methyl esters. J. Lipid Res. 10:589–592
     [Google Scholar]
  9. Citarella R. V., Colwell R. R. 1970; Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. J. Bacteriol. 104:434–442
     [Google Scholar]
  10. Colwell R. R. 1970; Polyphasic taxonomy of the genus Vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. J. Bacteriol. 104:410–433
     [Google Scholar]
  11. Davis B. R., Fanning G. R., Madden J. M., Stei-gerwalt A. G., Bradford H. B. Jr., Smith H. L. Jr, Brenner D. J. 1981; Characterization of biochemically atypical Vibrio cholerae strains and designation of a new pathogenic species, Vibrio mimicus . J. Clin. Microbiol. 14:631–639
     [Google Scholar]
  12. Goldfine H. 1972; Comparative aspects of bacterial lipids. Adv. Microb. Physiol. 8:2, 3, 22–27, 42
     [Google Scholar]
  13. Harwood C. S. 1978; Beneckea gazogenes sp. nov., a red, facultatively anaerobic, marine bacterium. Curr. Microbiol. 1:233–238
     [Google Scholar]
  14. Hickman F. W., Farmer J. J. III, Hollis D. G., Fanning G. R., Steigerwalt A. G., Weaver R. E., Brenner D. J. 1982; Identification of Vibrio hollisae sp. nov. from patients with diarrhea. J. Clin. Microbiol. 15:395–401
     [Google Scholar]
  15. Hisatsune K., Kondo S., Kawata T., Kishimoto Y. 1979; Fatty acid composition of lipopolysaccharide of Vibrio cholerae 35A3 (Inaba), NIH 90 (Ogawa), and 4715 (NAG). J. Bacteriol. 138:288–290
     [Google Scholar]
  16. Hollis D. G., Weaver R. E., Baker C. N., Thorns-berry C. 1976; Halophilic Vibrio species isolated from blood cultures. J. Clin. Microbiol. 3:425–431
     [Google Scholar]
  17. Huq M. I., Alam A. K. M. J., Brenner D. J., Morris G. K. 1980; Isolation of Vibrio-like group, EF-6, from patients with diarrhea. J. Clin. Microbiol. 11:621–624
     [Google Scholar]
  18. Kaper J. B., Lackman H., Colwell R. R., Joseph S. W. 1981; Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. J. Appl. Bacteriol. 50:359–377
     [Google Scholar]
  19. Kates M. 1964; Bacterial lipids. Adv. Lipid Res. 2:17–90
     [Google Scholar]
  20. Lechevalier M. P. 1982; Lipids in bacterial taxonomy. 444–445 495-507, 512, 516 Laskin A. I., Lechevalier H. A. CRC handbook of microbiology, 2. 4 CRC Press, Inc.; Boca Raton, Fla:
     [Google Scholar]
  21. Lee J. V., Donovan T. J., Furniss A. L. 1978; Characterization, taxonomy, and emended description of Vibrio metschnikovii . Int. J. Syst. Bacteriol. 28:99–111
     [Google Scholar]
  22. Lee J. V., Shread P., Furniss A. L., Bryant T. N. 1981; Taxonomy and description of Vibrio fluvialis, sp. nov. (synonym group F vibrios, group EF-6). J. Appl. Bacteriol. 50:73–95
     [Google Scholar]
  23. Love M., Teebken-Fisher D., Hose J. E., Farmer J. J. III, Hickman F. W., Fanning G. R. 1981; Vibrio damsela, a marine bacterium, causes skin ulcers on the damselfish Chromis punctipinnis . Science 214:1139–1140
     [Google Scholar]
  24. Machtiger N. A., O’Leary W. M. 1973; Fatty acid compositions of paracolons: Arizona, Citrobacter, and Providencia . J. Bacteriol. 114:80–85
     [Google Scholar]
  25. Moss C. W. 1978; New methodology for identification of nonfermentors: gas-liquid chromatographic chemotaxonomy. 188–195 Guardi G. L. Glucose nonfermeriting gram-negative bacteria in clinical microbiology CRC Press, Inc.; West Palm Beach, Fla:
     [Google Scholar]
  26. Moss C. W., Dees S. B. 1975; Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids. J. Chromatogr. 112:595–604
     [Google Scholar]
  27. O’Leary W. M. 1982; Lipoidal contents of specific microorganisms. 393–398 Laskin A. I., Lechevalier H. A. CRC handbook of microbiology, 2. 4 CRC Press, Inc.; Boca Raton, Fla:
     [Google Scholar]
  28. Oliver J. D., Colwell R. R. 1973; Extractable lipids of gram-negative marine bacteria: fatty acid composition. Int. J. Syst. Bacteriol. 23:442–458
     [Google Scholar]
  29. Reichelt J. L., Baumann P., Baumann L. 1976; Study of the genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch. Mikrobiol. 110:110–120
     [Google Scholar]
  30. Schiewe M. H., Trust T. J., Crosa J. H. 1981; Vibrio orciaia sp. nov.: a causative agent of vibriosis in fish. Curr. Microbiol. 6:343–348
     [Google Scholar]
  31. Shewan J. M., Veron M. 1974; Family II. Vibriona-ceae Veron 1965, 5245. 340–352 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  32. von Graevenitz A. 1980; Aeromonas and Plesiomonas . 220–225 Lennette E. H., Balows A., Hausler W. J., Truant J. P. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C.:
     [Google Scholar]
  33. Wachsmuth I. K., Morris G. K., Feeley J. C. 1980; Vibrio. 226–234 Lennette E. H., Balows A., Hausler W. J., Truant J. P. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C.:
     [Google Scholar]
  34. Yabuuchi E., Moss C. W. 1982; Cellular fatty acid composition of strains of three species of Sphingobacterium gen. nov. and Cytophagajohnsonae . FEMS Microbiol. Lett. 13:87–91
     [Google Scholar]
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Differentiation of Vibrionaceae Species by Their Cellular Fatty Acid Composition
Int J Syst Evol Microbiol 33, 777 (1983); https://doi.org/10.1099/00207713-33-4-777
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