@article{mbs:/content/journal/ijsem/10.1099/00207713-33-4-716, author = "SADOWSKY, MICHAEL J. and KEYSER, HAROLD H. and BOHLOOL, B. BEN", title = "Biochemical Characterization of Fast- and Slow-Growing Rhizobia That Nodulate Soybeans", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1983", volume = "33", number = "4", pages = "716-722", doi = "https://doi.org/10.1099/00207713-33-4-716", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-33-4-716", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Fast-growing, acid-producing soybean rhizobia were examined to determine their biochemical relatedness to each other, to typical slow-growing Rhizobium japonicum strains, and to other fast-growing species of Rhizobium. Although both the fast- and slow-growing soybean rhizobia were positive for catalase, urease, oxidase, nitrate reductase, and penicillinase, the fast-growing strains grouped with other fast-growing species of Rhizobium in that they tolerated 2% NaCl, were capable of growth at pH 9.5, utilized a large variety of carbohydrates (notably disaccharides), and produced serum zones in litmus milk. In addition, these fast-growing strains were similar to other fast-growing species of Rhizobium in that they produced appreciable levels of β-galactosidase and nicotinamide adenine dinucleotide phosphate-linked 6-phosphogluconate dehydrogenase but had no detectable hydrogenase activity. The fast-growing soybean rhizobia share symbiotic host specificity with Bradyrhizobium japonicum, but appear to be related biochemically to the other fast-growing species of Rhizobium.", }