1887

Abstract

Nine strains of motile, anaerobic, nonsporeforming, gram-negative bacteria were isolated from human lesions of adult and juvenile periodontitis and compared with type strains ATCC 29863, ATCC 33150, ATCC 12561, and ATCC 29359. The cells of our isolates were long and serpentine and appeared to be bilaterally flagellated when they were examined by dark-field microscopy, giving the cells a centipede-like appearance. Electron microscopic studies snowed the presence of a linear zone of flagellar insertions which spiraled around the cell body. Cultures were fermentative and produced propionic, acetic, lactic, and succinic acids. The guanine-plus-cytosine contents of the deoxyribonucleic acids of three isolates ranged from 52 to 54 mol%, as determined by the thermal denaturation method. All strains had 58 to 100% deoxyribonucleic acid homology with one another, but only 14% or less homology with the deoxyribonucleic acids of three previously described species. We propose the name gen. nov., sp. nov. for these organisms; strain LL2383 (= ATCC 35019) is the type strain.

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1983-07-01
2022-08-10
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References

  1. Bryant M. P. 1974; Genus Selenomonas, . 424–426 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  2. Crosa J. H., Brenner D. T., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes.. J. Bacteriol 115:904–911
    [Google Scholar]
  3. De Ley J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek. J. Microbiol. Serol 36:461–474
    [Google Scholar]
  4. Edelman M. 1975; Purification of DNA by affinity chromatography: removal of polysaccharide contaminants. Anal. Biochem 65:293–297
    [Google Scholar]
  5. Gins H. A. 1934; Die nichtversporenden Anaerobier der Mundhohle und der Zahne. Zentralbl. Bakteriol. Parasitenkd. Infektionskr 132:129–145
    [Google Scholar]
  6. Hofstad T., Aasjord P. 1982; Eubacterium plautii (Seguin 1928) comb. nov. Int. J. Syst. Bacteriol 32:346–349
    [Google Scholar]
  7. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. , 4. Virginia Polytechnic Institute and State University Anaerobe Laboratory; Blacksburg:
    [Google Scholar]
  8. Holdeman L. V., Moore W. E. C. 1974; Family I. Bacteroidaceae, . 384 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Karnovsky M. J. 1965; A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy.. J. Cell Biol 27:137A–138A
    [Google Scholar]
  10. Kritchevsky B., Séguin P. 1921; Traitement des spirochétoses buccales (pyorrhée alveolaire) par les préparations arsenicales. L’Odontologie 59:720–742
    [Google Scholar]
  11. Lee S. Y., Mabee M. S., Jangaard N. O. 1978; Pectinatus, a new genus of the family Bacteroidaceae. . Int. J. Syst. Bacteriol 28:582–594
    [Google Scholar]
  12. Lewis C. T., Short C. 1966; Latin dictionary. Oxford University Press; London:
    [Google Scholar]
  13. Luft J. H. 1961; Improvement in epoxy resin embedding methods.. J. Biophys. Biochem. Cytol 9:409–414
    [Google Scholar]
  14. Mandel M., Igambi L., Bergendahl J., Dodson M. L. Jr., Scheltgen E. 1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid.. J. Bacteriol 101:333–338
    [Google Scholar]
  15. Manganiello A. D., Socransky S. S., Smith C., Propas D., Oram V., Dogon I. L. 1977; Attempt to increase viable count recovery of human supragingival dental plaque.. J. Periodontal Res 12:107–119
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms.. J. Mol. Biol 3:208–218
    [Google Scholar]
  17. Moore W. E. C., Holdeman L. V. 1974; Genus II. Fusobacterium, . 404–416 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  18. Moore W. E. C., Holdeman L. V., Smibert R. M., Hash D. E., Burmeister J. A., Ranney R. R. 1982; Bacteriology of severe periodontitis in young adult humans. Int. J. Sys. Bacteriol 38:1137–1148
    [Google Scholar]
  19. Mühlens P. 1909; Uber Züchtung von anaëroben Mikroorganismen der Mundhöhle (u.a. Spirillum sputigenum) . Zentralbl. Bakteriol. Parasitenkd. Infektionskr 48:523–528
    [Google Scholar]
  20. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.. J. Cell Biol 17:208–212
    [Google Scholar]
  21. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.. J. Mol. Biol 113:237–251
    [Google Scholar]
  22. Salanitro J. P., Muirhead P. A. 1975; Quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. Appl. Microbiol 29:374–381
    [Google Scholar]
  23. Sebald M. 1962; Etude sur les bactéries anaérobies gram-négatives asporulées. Imprimerie Barneoud, S.A.; Laval, France:
    [Google Scholar]
  24. Séguin P. 1928; Culture du Fusobacterium plauti, forme mobile du bacille fusiforme. C. R. Seances Soc. Biol. Paris 99:439–442
    [Google Scholar]
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