1887

Abstract

Four strains of obligately anaerobic spirochetes were isolated from marine intertidal mud. These strains grew to yields of 2 × 10 to 6 × 10 cells per ml in chemically defined media containing glucose, amino acids or NHCl, sulfide, vitamins, and inorganic salts. These spirochetes required relatively high levels of Na for growth and utilized carbohydrates but not amino acids as growth substrates. Glucose was fermented to CO, H, ethanol, and acetate. These four strains also formed the branched-chain fatty acids isovalerate , 2-methylbutyrate, and isobutyrate as fermentation products of -leucine, -isoleucine, and -valine, respectively. The adenosine 5'-triphosphate generated via fermentation of these amino acids was expended by the spirochetes to prolong survival during periods of growth substrate starvation. The deoxyribonucleic acids of the four strains contained 63.6 to 65.6 mol% guanine plus cytosine. We concluded that these four strains are representatives of a new species, for which we propose the name the type strain of this species is strain MA-2 (= DSM 2461).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-33-3-573
1983-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/33/3/ijs-33-3-573.html?itemId=/content/journal/ijsem/10.1099/00207713-33-3-573&mimeType=html&fmt=ahah

References

  1. Blakemore R. P., Canale-Parola E. 1973; Morphological and ecological characteristics of Spirochaeta plicatilis. . Arch. Mikrobiol 89:273–289
    [Google Scholar]
  2. Breznak J. A., Canale-Parola E. 1975; Morphology and physiology of Spirochaeta aurantia strains isolated from aquatic habitats. Arch. Microbiol 105:1–12
    [Google Scholar]
  3. Canale-Parola E. 1974; Genus I. Spirochaeta Ehrenberg 1835, 313. 168–171 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. the Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Canale-Parola E. 1977; Physiology and evolution of spirochetes. Bacteriol. Rev 41:181–204
    [Google Scholar]
  5. Canale-Parola E. 1978; Motility and chemotaxis of spirochetes. Annu. Rev. Microbiol 32:69–99
    [Google Scholar]
  6. Canale-Parola E., Holt S. C., Udris Z. 1967; Isolation of free-living anaerobic spirochetes. Arch. Mikrobiol 59:41–48
    [Google Scholar]
  7. Canale-Parola E., Udris Z., Mandel M. 1968; The classification of free-living spirochetes. Arch. Mikrobiol 63:385–397
    [Google Scholar]
  8. Greenberg E. P., Canale-Parola E. 1976; Spirochaeta halophila sp. n., a facultative anaerobe from a high-salinity pond. Arch. Microbiol 110:185–194
    [Google Scholar]
  9. Harwood C. S., Canale-Parola E. 1981; Branched-chain amino acid fermentation by a marine spirochete: strategy for starvation survival.. J. Bacteriol 148:109–116
    [Google Scholar]
  10. Harwood C. S., Canale-Parola E. 1981; Adenosine 5'-triphosphate-yielding pathways of branched-chain amino acid fermentation by a marine spirochete.. J. Bacteriol 148:117–123
    [Google Scholar]
  11. Harwood C. S., Canale-Parola E. 1982; Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete.. J. Bacteriol 152:246–254
    [Google Scholar]
  12. Hespell R. B., Canale-Parola E. 1970; Spirochaeta litoralis sp. n., a strictly anaerobic marine spirochete. Arch. Mikrobiol 74:1–18
    [Google Scholar]
  13. Hespell R. B., Canale-Parola E. 1970; Carbohydrate metabolism in Spirochaeta stenostrepta. . J. Bacteriol 103:216–226
    [Google Scholar]
  14. MacLeod R. A. 1965; The question of the existence of specific marine bacteria. Bacteriol. Rev 29:9–23
    [Google Scholar]
  15. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the gua-nine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  16. Paster B. J., Canale-Parola E. 1982; Physiological diversity of rumen spirochetes. Appl. Environ. Microbiol 43:686–693
    [Google Scholar]
  17. Reichelt J. L., Baumann P. 1974; Effect of sodium chloride on growth of heterotrophic marine bacteria. Arch. Microbiol 97:329–345
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. 1981; General characterization. 409–443 Gerhardt P. Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  19. Weber F. H., Greenberg E. P. 1981; Rifampin as a selective agent for the enumeration and isolation of spiro-chetes from salt marsh habitats. Curr. Microbiol 5:303–306
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-33-3-573
Loading
/content/journal/ijsem/10.1099/00207713-33-3-573
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error