1887

Abstract

A mycoplasma designated strain R171 (type strain) was isolated from the infraorbital sinus of an adult chicken. This organism was assigned to the class and the order on the basis of its morphological, physical, and cultural characteristics. Its deoxyribonucleic acid base composition was 24.5 mol% guanine plus cytosine. Strain R171 was sterol dependent, and since it did not produce helical forms or hydrolyze urea, it was assigned to the family . Strain R171 fermented glucose, hydrolyzed arginine, and produced films and spots. This strain was shown to be serologically distinct from 74 currently accepted mycoplasma species or serovars by growth inhibition, immunofluorescence, and immunodiffusion tests, which were supported in some cases by metabolism inhibition tests. Thus, strain R171 appears to be a new and distinct mycoplasma species, for which we propose the name (= NCTC 10191 = ATCC 35015).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-33-2-329
1983-04-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/33/2/ijs-33-2-329.html?itemId=/content/journal/ijsem/10.1099/00207713-33-2-329&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J.E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol 20:35–58
    [Google Scholar]
  2. Anderson D. R., Barile M.F. 1965; Ultrastructure of Mycoplasma hominis . J. Bacteriol 90:180–192
    [Google Scholar]
  3. Aycardi E. R., Anderson D. P., Hanson R.P. 1971; Classification of avian mycoplasmas by gel-diffusion and growth-inhibition tests. Avian Dis 15:434–447
    [Google Scholar]
  4. Barber T. L., Fabricant J. 1971; A suggested reclassification of avian mycoplasma serotypes. Avian Dis 15:125–128
    [Google Scholar]
  5. Black F. T. 1973; Modifications of the growth inhibition test and its application to human T-mycoplasmas. Appl Microbiol 25:528–533
    [Google Scholar]
  6. Bradbury J. M. 1977; Rapid biochemical tests for characterization of the Mycoplasmatales.. J. Clin. Microbiol 5:531–534
    [Google Scholar]
  7. Bradbury J. M., Jordan F.T.W. 1971; The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests. J. Hyg 69:593–606
    [Google Scholar]
  8. Bradbury J. M., McCarthy J. 1981; Rupture of the digital flexor tendons of chickens after infection with Mycoplasma iowae . Vet. Rec 109:428–429
    [Google Scholar]
  9. Bradbury J. M., Oriel C. O., Jordan F.T.W. 1976; Simple method for immunofluorescent identification of mycoplasma colonies. J. Clin. Microbiol 3:449–452
    [Google Scholar]
  10. Edward D., ff G. 1971; Determination of sterol requirement for Mvcoplasmatales. J. Gen. Microbiol 69:205–210
    [Google Scholar]
  11. Edward D., ff G., Moore W.B. 1975; A method for determining the utilisation of glucose by mycoplasmas. J. Med. Microbiol 8:451–454
    [Google Scholar]
  12. Ernø H., Al-Aubaidi J. M., Ojo M. O., Minga U. M., Sikdar A. 1978; Classification and identification of ovine and caprine mycoplasmas. Acta Vet. Scand 19:392–406
    [Google Scholar]
  13. Ernø H., Stipkovits L. 1973; Bovine mycoplasmas. Cultural and biochemical studies I. Acta Vet. Scand 14:436–449
    [Google Scholar]
  14. Freundt E. A., Andrews B. E., Erne H., Kunze M., Black F.T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanetholsulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 255:104–112
    [Google Scholar]
  15. Freundt E. A., Erne H., Lemcke R.M. 1979; Identification of mycoplasmas. 377–434 Bergan T., Norris J.R. Methods in microbiology 13 Academic Press, Inc; London:
    [Google Scholar]
  16. Frey M. L., Hawk S. T., Hale P.A. 1972; A division by microcomplement fixation test of previously reported avian mycoplasma serotypes into identification groups. Avian Dis 16:780–792
    [Google Scholar]
  17. Friis N. F. 1975; Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare. Nord. Veterinaermed 27:337–339
    [Google Scholar]
  18. Hayflick L. 1965; Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med 23:285–303
    [Google Scholar]
  19. Hollingdale M. R., Lemcke R.M. 1969; The antigens of Mycoplasma hominis.. J. Hyg 67:585–602
    [Google Scholar]
  20. Howard C. J., Brownlie J., Gourlay R. N., Collins J. 1975; Presence of a dialysable fraction in normal bovine whey capable of killing several species of bovine mycoplasmas. J. Hyg 74:261–270
    [Google Scholar]
  21. Howard C. J., Gourlay R.N. 1973; Inhibition by normal rabbit sera of growth of T-mycoplasma strains isolated from different animal species. J. Gen. Microbiol 78:277–285
    [Google Scholar]
  22. International Committee on Systematic Bacteriology Sub-committee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol 29:172–180
    [Google Scholar]
  23. Jordan F. T. W., Amin M. 1980; A survey of mycoplasma infections in domestic poultry. Res. Vet. Sci 28:96–100
    [Google Scholar]
  24. Kellenberger E., Ryter A., Sechaud J. 1958; Electron microscope study of DNA-containing plasma. J. Biophys. Biochem. Cytol 4:671–678
    [Google Scholar]
  25. Kelton W. H., Mandel M. 1969; Deoxyribonucleic acid base compositions of mycoplasma strains of avian origin. J. Gen. Microbiol 56:131–135
    [Google Scholar]
  26. Kirby K. S. 1957; A new method for the isolation of deoxyribonucleic acids: evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem. J 66:495–504
    [Google Scholar]
  27. Langford E. V., Leach R.H. 1973; Characterization of a mycoplasma isolated from infectious bovine keratoconjunctivitis: M. bovoculi sp. nov. Can. J. Microbiol 19:1435–1444
    [Google Scholar]
  28. Lynn R. J. 1967; The immune response of rabbits to various strains of mycoplasma. Ann. N.Y. Acad. Sci 143:654–663
    [Google Scholar]
  29. McClenaghan M., Bradbury J. M., Howse J.N. 1981; Embryo mortality associated with avian mycoplasma serotype I. Vet. Rec 108:459–460
    [Google Scholar]
  30. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R.M. 1966; A color test for the measurement of antibody to the non-acid forming human Mycoplasma species. Am. J. Epidemiol 84:51–66
    [Google Scholar]
  31. Razin S. 1969; Structure and function in mycoplasmas. Annu. Rev. Microbiol 23:317–356
    [Google Scholar]
  32. Rhoades K. R. 1981; Pathogenicity of strains of the IJKNQR group of avian mycoplasmas for turkey embryos and poults. Avian Dis 25:104–111
    [Google Scholar]
  33. Roberts D. H. 1971; Interaction of porcine mycoplasmas with fresh animal serum. J. Hyg 69:361–368
    [Google Scholar]
  34. Rosendal S., Black F.T. 1972; Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta Pathol. Microbiol. Scand. Sect. B 80:615–622
    [Google Scholar]
  35. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–443
    [Google Scholar]
  36. Shepard M. C. 1973; Differential methods for identification of T-mycoplasmas based on demonstration of urease. J. Infect. Dis 127:S22–S25
    [Google Scholar]
  37. Stipkovits L., El-Ebeedy A. A. 1977; Biochemical and serological studies of avian mycoplasmas. Zentralbl. Veterinaermed. Reihe B 24:218–230
    [Google Scholar]
  38. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R.M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg 64:91–104
    [Google Scholar]
  39. Williams C. O., Wittier R.G. 1971; Hydrolysis of esculin and phosphatase production by members of the order Mycoplasmatales which do not require sterol. Int. J. Syst. Bacteriol 21:73–77
    [Google Scholar]
  40. Yoder H. W. Jr., Hofstad M.S. 1962; A previously unreported serotype of avian mycoplasma. Avian Dis 6:147–160
    [Google Scholar]
  41. Yoder H. W. Jr., Hofstad M.S. 1964; Characterization of avian mycoplasma. Avian Dis 8:481–512
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-33-2-329
Loading
/content/journal/ijsem/10.1099/00207713-33-2-329
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error