@article{mbs:/content/journal/ijsem/10.1099/00207713-33-2-285, author = "TSANG, ANNA Y. and DRUPA, ILGA and GOLDBERG, MIRTA and McCLATCHY, J. KENNETH and BRENNAN*, PATRICK J.", title = "Use of Serology and Thin-Layer Chromatography for the Assembly of an Authenticated Collection of Serovars Within the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum Complex", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1983", volume = "33", number = "2", pages = "285-292", doi = "https://doi.org/10.1099/00207713-33-2-285", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-33-2-285", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The seroagglutination test devised by Schaefer (Methods Microbiol. 13:323-344,1980) and the thin-layer chromatography procedure of Brennan et al. (J. Clin. Microbiol. 8:374–379,1978; 15:447–455,1982) were used to assemble an authenticated collection composed of 30 of the 31 known serovars of the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex, and from these serovars a new set of absorbed specific homologous rabbit antisera was prepared. Isolates of a few serovars (serovars 18, 41, and 43) showed the expected seroagglutination reactions but also produced several thin-layer Chro-matographie profiles, a phenomenon which places further emphasis on the need to use both thin-layer chromatography and serological analyses when the epidemio-logical aspects of nontuberculous mycobacteria are examined.", }