The seroagglutination test devised by Schaefer (Methods Microbiol. :23–344, 1980) and the thin-layer chromatography procedure of Brennan et al. (J. Clin. Microbiol. :374–379, 1978; :447–455, 1982) were used to assemble an authenticated collection composed of 30 of the 31 known serovars of the complex, and from these serovars an new set of absorbed specific homologous rabbit antisera was prepared. Isolates of a few serovars (serovars 18, 41, and 43) showed the expected seroagglutination reactions but also produced several thin-layer chromatographic profiles, a phenomenon which places further emphasis on the need to use both thin-layer chromatography and serological analyses when the epidemiological aspects of nontuberculous mycobacteria are examined.


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