1887

Abstract

The seroagglutination test devised by Schaefer (Methods Microbiol. 13:323-344,1980) and the thin-layer chromatography procedure of Brennan et al. (J. Clin. Microbiol. 8:374–379,1978; 15:447–455,1982) were used to assemble an authenticated collection composed of 30 of the 31 known serovars of the complex, and from these serovars a new set of absorbed specific homologous rabbit antisera was prepared. Isolates of a few serovars (serovars 18, 41, and 43) showed the expected seroagglutination reactions but also produced several thin-layer Chro-matographie profiles, a phenomenon which places further emphasis on the need to use both thin-layer chromatography and serological analyses when the epidemio-logical aspects of nontuberculous mycobacteria are examined.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-33-2-285
1983-04-01
2022-09-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/33/2/ijs-33-2-285.html?itemId=/content/journal/ijsem/10.1099/00207713-33-2-285&mimeType=html&fmt=ahah

References

  1. Barrow W. W., Ullom B. P., Brennan P. J. 1980; Peptidoglycolipid nature of the superficial cell wall sheath of smooth-colony-forming mycobacteria. J. Bacteriol 144:814–822
    [Google Scholar]
  2. Boisvert H., Truffot C. 1979; Relations entre Mycobacterium simiae et le complexe M. avium-intracellulare-scrofulaceum . Ann. Microbiol Paris: 130B457–466
    [Google Scholar]
  3. Brennan P. J. 1981; Structures of the typing antigens of atypical mycobacteria. A brief review of present knowledge. Rev. Infect. Dis 3:905–913
    [Google Scholar]
  4. Brennan P. J., Aspinall G. O., Nam Shin J. E. 1981; Structure of the specific oligosaccharides from the glyco-peptidolipid antigens of serovars in the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex. J. Biol. Chem 256:6817–6822
    [Google Scholar]
  5. Brennan P. J., Goren M. B. 1979; Structural studies on the type-specific antigens and lipids of the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum sero-complex. J. Biol. Chem 254:4205–4211
    [Google Scholar]
  6. Brennan P. J., Heifets M., Ullom B. P. 1982; Thin-layer chromatography of lipid antigens as a means of identifying nontuberculous mycobacteria. J. Clin. Microbiol 15:447–455
    [Google Scholar]
  7. Brennan P. J., Mayer H., Aspinall G. O., Nam Shin J. E. 1980; Structures of the glycopeptidolipid antigens from serovars in the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum serocomplex. Eur. J. Biochem 115:7–15
    [Google Scholar]
  8. Brennan P. J., Souhrada M., Ullom B., McClatchy J. K., Goren M. B. 1978; Identification of atypical mycobacteria by thin-layer chromatography of their surface antigens. J. Clin. Microbiol 8:374–379
    [Google Scholar]
  9. Conn M. L., Waggoner R. F., McClatchy J. K. 1968; The 7H11 media for the cultivation of mycobacteria. Am. Rev. Respir. Dis 98:295–296
    [Google Scholar]
  10. Davidson P. T. 1979; The management of disease with atypical mycobacteria. Clin. Notes Respir. Dis 18:3–13
    [Google Scholar]
  11. Jenkins P. A. 1981; Lipid analysis in the identification of mycobacteria—an appraisal. Rev. Infect. Dis 3:862–866
    [Google Scholar]
  12. Jenkins P. A., Marks J., Schaefer W. B. 1972; Thin-layer chromatography of mycobacterial lipids as an aid to classification: the scotochromogenic mycobacteria, including Mycobacterium scrofulaceum, M. xenopi, M. aquae, M. gordonae, M. flavescens . Tubercle 53:118–127
    [Google Scholar]
  13. Marks J., Jenkins P. A., Schaefer W. B. 1969; Identification and incidence of a third type of Mycobacterium avium . Tubercle 50:394–395
    [Google Scholar]
  14. Marks J., Jenkins P. A., Tsukamura M. 1972; Mycobacterium szulgae—a new pathogen. Tubercle 53:210–214
    [Google Scholar]
  15. Marks J., Szulga T. 1965; Thin-layer chromatography of mycobacterial lipids as an aid to classification: technical procedures. Mycobacterium fortuitum. Tubercle 46:400–411
    [Google Scholar]
  16. McClatehy J. K. 1981; The seroagglutination test in the study of nontuberculous mycobacteria. Rev. Infect. Dis 3:867–870
    [Google Scholar]
  17. National Institutes of Health 1980; Mycobacterial culture collection. Publication 80-289. National Institutes of Health, Bethesda; Md:
    [Google Scholar]
  18. Schaefer W. B. 1965; Serologie identification and classification of the atypical mycobacteria by their agglutination. Am. Rev. Respir. Dis 92: Suppl. 85–93
    [Google Scholar]
  19. Schaefer W. B. 1967; Serologie identification of the atypical mycobacteria and its value in epidemiologic studies. Am. Rev. Respir. Dis 96:115–118
    [Google Scholar]
  20. Schaefer W. B. 1967; Type-specificity of atypical mycobacteria in agglutination and antibody absorption tests. Am. Rev. Respir. Dis 96:1165–1168
    [Google Scholar]
  21. Schaefer W. B. 1980; Serological identification of atypical mycobacteria. Methods Microbiol 13:323–344
    [Google Scholar]
  22. Szulga T., Jenkins P. A., Marks J. 1966; Thin-layer chromatography of mycobacterial lipids as an aid to classification: Mycobacterium kansasii and Mycobacterium marinum . Tubercle 47:130–136
    [Google Scholar]
  23. Vestal A. 1975 Procedures for the isolation and identification of mycobacteria. Publication (CDC)77–8230 Centers for Disease Control; Atlanta, Ga:
    [Google Scholar]
  24. Wolinsky E. 1979; Nontuberculous mycobacteria and associated diseases. Am. Rev. Respir. Dis 119:107–159
    [Google Scholar]
  25. Wolinsky E., Schaefer W. B. 1973; Proposed numbering scheme for mycobacterial serotypes by agglutination. Int. J. Syst. Bacteriol 23:182–183
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-33-2-285
Loading
/content/journal/ijsem/10.1099/00207713-33-2-285
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error