Since the species classified in order can be separated into at least six antigenically distinct groups by analytical serology, we compared the antigenic specificities of the adenosine triphosphatases (ATPases) and reduced nicotinamide adenine dinucleotide dehydrogenases of 14 strains by using quantitative immunoelectrophoresis and specific strains to identify enzymatically active precipitin peaks. The following species and serological groups were studied: , and species bovine group VII (group 1); and (group 2); (group 4); (group 5); (group 6); , and (group 7); and (ungrouped). Each strain showed ATPase activity which formed a precipitin peak against the homologous antiserum. Eight serologically distinct ATPases were identified, and most of these ATPases cross-reacted only within serologically related clusters of species, not between clusters; the exception was group 7, where the ATPase of had a different specificity than the cross-reacting enzymes of and . All species except possessed a reduced nicotinamide adenine dinucleotide dehydrogenase, but the enzymes in , and did not precipitate with any antisera. The remaining species showed five distinct specificities of reduced nicotinamide adenine dinucleotide dehydrogenases, and the antigenic relationships of these enzymes exactly paralleled those observed with ATPases. Thus, the serological specificities of common mycoplasmic enzymes are powerful taxonomic tools.


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