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Volume 33, Issue 2

Distinctive Antigenic Specificities of Adenosine Triphosphatases and Reduced Nicotinamide Adenine Dinucleotide Dehydrogenases as Means for Classification of the Order Free

Abstract

Since the species classified in order can be separated into at least six antigenically distinct groups by analytical serology, we compared the antigenic specificities of the adenosine triphosphatases (ATPases) and reduced nicotinamide adenine dinucleotide dehydrogenases of 14 strains by using quantitative immunoelectrophoresis and specific strains to identify enzymatically active precipitin peaks. The following species and serological groups were studied: , and species bovine group VII (group 1); and (group 2); (group 4); (group 5); (group 6); , and (group 7); and (ungrouped). Each strain showed ATPase activity which formed a precipitin peak against the homologous antiserum. Eight serologically distinct ATPases were identified, and most of these ATPases cross-reacted only within serologically related clusters of species, not between clusters; the exception was group 7, where the ATPase of had a different specificity than the cross-reacting enzymes of and . All species except possessed a reduced nicotinamide adenine dinucleotide dehydrogenase, but the enzymes in , and did not precipitate with any antisera. The remaining species showed five distinct specificities of reduced nicotinamide adenine dinucleotide dehydrogenases, and the antigenic relationships of these enzymes exactly paralleled those observed with ATPases. Thus, the serological specificities of common mycoplasmic enzymes are powerful taxonomic tools.

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Copyright © 1983 International Union of Microbiological Societies
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1983-04-01
2024-04-25
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References

  1. Adler H. E., Fabricant J., Yamomoto R., Berg J. 1958; Symposium on chronic respiratory diseases of poultry. I. Isolation and identification of pleuropneumonia-like organisms of avian origin. Am. J. Vet. Res. 19:440–447
     [Google Scholar]
  2. Alexander A. G., Kenny G. E. 1978; Application of charge shift electrophoresis to antigenic analysis of mycoplasmic membranes by two-dimensional (crossed) immunoelectrophoresis. Infect. Immun 20:483–489
     [Google Scholar]
  3. Alexander A. G., Kenny G. E. 1980; Characterization of the strain-specific and common surface antigens of Mycoplasma arginini. Infect. Immun 29:442–451
     [Google Scholar]
  4. Ashani Y., Catravas G. N. 1980; Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Anal. Biochem 109:55–62
     [Google Scholar]
  5. Axelsen N. H., Bock E. 1972; Identification and quantitation of antigens and antibodies by means of quantitative immunoelectrophoresis. A survey of methods. J. Immunol. Methods 1:109–121
     [Google Scholar]
  6. Cole B. C., Golightly L., Ward J. R. 1967; Characterization of mycoplasma strains from cats. J. Bacteriol 94:1451–1458
     [Google Scholar]
  7. Cottew G. S., Leach R. S. 1969; Mycoplasmas of cattle, sheep and goats. 527–570 Hayflick L. The Mycoplasma tales and the L-phase of bacteria Appleton-Century-Crofts; New York:
     [Google Scholar]
  8. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science 130:432–437
     [Google Scholar]
  9. Edward D. G. ff. 1954; The pleuropneumonia group of organisms: a review, together with some new observations. J. Gen. Microbiol 10:27–64
     [Google Scholar]
  10. Edward D. G. ff., Freundt E. A. 1969; Proposal for classifying organisms related to Mycoplasma laidlawii in a family Sapromycetaceae, genus Sapromyces, with the Mycoplasmatales. J. Gen. Microbiol 57:391–395
     [Google Scholar]
  11. Edward D. G. ff., Freundt E. A. 1970; Amended nomenclature for strains related to Mycoplasma laidlawii. J. Gen. Microbiol 62:1–2
     [Google Scholar]
  12. Fiala M., Kenny G. E. 1966; Enhancement of rhinovirus plaque formation in human heterophoid cell cultures by magnesium and calcium. J. Bacteriol 92:1710–1715
     [Google Scholar]
  13. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J., Zablen L. B., Blakemore R., Gupta R., Bonen L., Lewis B. J., Stahl D. A., Luehrsen K. R., Chen K. N., Woese C. R. 1980; The phylogeny of prokaryotes. Science 209:457–463
     [Google Scholar]
  14. Freundt E. A. 1955; A suggested classification and no-menclature for organisms of the pleuropneumonia group. Int. Bull. Bacteriol. Nomencl. Taxon 5:85–93
     [Google Scholar]
  15. Freundt E. A., Edward D. G. ff. 1979; Classification and taxonomy. 1–41 Barile M. F., Razin S. The mycoplasmas 1 Academic Press, Inc; New York:
     [Google Scholar]
  16. Hahn R. G., Kenny G. E. 1974; Differences in arginine requirement for growth among arginine-utilizing Mycoplasma species. J. Bacteriol 117:611–618
     [Google Scholar]
  17. Harris H., Hopkinson D. A. 1976; Handbook of enzyme electrophoresis in human genetics. North-Holland Publishing Co; Amsterdam:
     [Google Scholar]
  18. Heienius A., Simons K. 1977; Charge shift electrophoresis: a simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution. Proc. Natl. Acad. Sci.U. S. A 74529–532
     [Google Scholar]
  19. Kaplan A. E., Weiss E. R., Byrne S. T., El-Torkey N. M., Margolis S. A. 1981; Purified reduced nicotinamide adenine dinucleotide: responses to lactate dehydrogenase isozymes from three cell sources. Science . 212:553–554
     [Google Scholar]
  20. Kenny G. E. 1967; Heat stability and organic solvent solubility of mycoplasma antigens. Ann. N. Y. Acad. Sci 143:676–681
     [Google Scholar]
  21. Kenny G. E. 1969; Serological comparison of ten glycolytic Mycoplasma species. J. Bacteriol 98:1044–1055
     [Google Scholar]
  22. Kenny G. E. 1971; Immunogenicity of Mycoplasma pneumoniae. Infect. Immun 3:510–515
     [Google Scholar]
  23. Kenny G. E. 1977; Antigenic analysis of the Mycoplasmatales. 376–382 Hobson H., Holmes K. Nongonococcal urethritis and related infections American Society of Microbiology; Washington, D. C.:
     [Google Scholar]
  24. Kenny G. E. 1979; Antigenic determinants. 351–384 Barile M. F., Razin S. The mycoplasmas 1 Academic Press, Inc; New York:
     [Google Scholar]
  25. Kenny G. E., Cartwright F. D. 1977; Effect of urea concentration on growth of Ureaplasma urealyticum (T-strain mycoplasma). J. Bacteriol 132:144–150
     [Google Scholar]
  26. Kenny G. E., Grayston J. T. 1965; Eaton pleuro-pneumonia-like organism (Mycoplasma pneumonia) complement-fixing antigen: extraction with organic solvents. J. Immunol 95:19–25
     [Google Scholar]
  27. Kirchhoff H. 1978; Acholeplasma equifetale and Acholeplasma hippikon, two new species from aborted horse fetuses. Int. J. Syst. Bacteriol 28:76–81
     [Google Scholar]
  28. Klieneberger E. 1940; The pleuropneumonia-like organisms. Further comparative studies and a descriptive account of recently discovered types. J. Hyg 40:204–222
     [Google Scholar]
  29. Klotzsch S. G., Klotzsch H. R. 1969; Inhibitor-contaminated NADH: its influence on dehydrogenases and dehydrogenase-coupled reaction. S. Clin. Chem 15:1056–1061
     [Google Scholar]
  30. Larraga V., Razin S. 1976; Reduced nicotinamide adenine dinucleotide oxidase activity in membranes and cytoplasm of Acholeplasma laidlawii and Mycoplasma mycoides subsp capri.. J. Bacteriol 128:827–833
     [Google Scholar]
  31. Laurell C. B. 1965; Antigen-antibody crossed electrophoresis. Anal. Biochem 10:358–361
     [Google Scholar]
  32. Lee G. Y., Kenny G. E. 1983; Common cytoplasmic antigen in five Acholeplasma species. Int. J. Syst. Bacteriol 33:358–365
     [Google Scholar]
  33. Lemcke R. M. 1973; Serological reactions of mycoplasmas. Ann. N. Y. Acad. Sci 225:46–53
     [Google Scholar]
  34. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin reagent. J. Biol. Chem 193:265–275
     [Google Scholar]
  35. Margolis S. A., Howell B. F., Schaffer R. 1976; Purification and analysis of the purity of NADH. Clin. Chem 22:1322–1329
     [Google Scholar]
  36. Mclvor R. S., Kenny G. E. 1978; Differences in incorporation of nucleic acid bases and nucleosides by various Mycoplasma and Acholeplasma species. J. Bacteriol 135:483–489
     [Google Scholar]
  37. Neimark H. C. 1970; Division of mycoplasmas into subgroups. J. Gen. Microbiol 63:249–263
     [Google Scholar]
  38. O’Brien S. J., Simonson J. M., Grabowski M. W., Barile M. F. 1981; Analysis of multiple isoenzyme expression among twenty-two species of Mycoplasma and Acholeplasma. J. Bacteriol 146:222–232
     [Google Scholar]
  39. Owen P., Salton M. R. J. 1975; Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis. Proc. Natl. Acad. Sci.U. S. A 723711–3715
     [Google Scholar]
  40. Pollack J. D., Razin S., Cleverdon R. C. 1965; Localization of enzymes in Mycoplasma. J. Bacteriol 90:617–622
     [Google Scholar]
  41. Shepard M. C., Lunsford C. D., Ford D. K., Purcell R. H., Taylor-Robinson D., Razin S., Black F. T. 1974; Ureaplasma urealyticum gen. nov., sp. nov.: proposed nomenclature for the human T (T-strain) mycoplasmas. Int. J. Syst. Bacteriol 24:160–171
     [Google Scholar]
  42. Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol 29:172–180
     [Google Scholar]
  43. Sueoka N. 1961; Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data. J. Mol. Biol 3:31–40
     [Google Scholar]
  44. Sugino W. M. 1980; Heterogeneity of thymidine kinase among species of Mycoplasma and Acholeplasma. Int. J. Syst. Bacteriol 30:103–105
     [Google Scholar]
  45. Thirkill C. E., Kenny G. 1974; Serological comparison of five arginine-utilizing Mycoplasma species by twodimensional immunoelectrophoresis. Infect. Immun 10:624–632
     [Google Scholar]
  46. Tully J. G., Barile M. F., Edward D. G. ff., Theodore T. S., Emo H. 1974; Characterization of some caprine mycoplasmas, with proposals for new species, M. capricolum and M. putrefaciens. J. Gen. Microbiol.102–120
     [Google Scholar]
  47. Tully J. G., Del Giudice R. A., Barile M. F. 1972; Synonomy of Mycoplasma arginini and Mycoplasma leonis. Int. J. Syst. Bacteriol 22:47–55
     [Google Scholar]
  48. Weeke B. 1973; Equipment, reagents, procedures. 37–46 Axelsen H., Kroll J., Weeke B. A manual of quantitative immunoelectrophoresis: methods and applications Universitetsforlaget; Oslo:
     [Google Scholar]
  49. Wienme R. J. 1965; Special problems. 94–113 Agar gel electrophoresis; Elsevier, Amsterdam:
     [Google Scholar]
  50. Whittler R. G., Cary S. G., Lindberg R. B. 1956; Reversion of a pleuropneumonia-like organism to a Corynebacterium during tissue culture passage. J. Gen. Microbiol 14:763–775
     [Google Scholar]
  51. Woese C. R., Maniloff J., Zablen L. B. 1980; Phylo-genetic analysis of the mycoplasmas. Proc. Natl. Acad. Sci.U. S. A 77494–498
     [Google Scholar]
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Distinctive Antigenic Specificities of Adenosine Triphosphatases and Reduced Nicotinamide Adenine Dinucleotide Dehydrogenases as Means for Classification of the Order Mycoplasmatales
Int J Syst Evol Microbiol 33, 218 (1983); https://doi.org/10.1099/00207713-33-2-218
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