Thirteen strains representing three named species, , and , were examined for the presence of 30 enzymes by horizontal starch gel electrophoresis. A total of 29 enzymes were demonstrated. Twelve of these enzymes have not been reported previously in acholeplasmas (alcohol dehydrogenase, aldolase, alkaline phosphatase, arginase, arginine deiminase, carbarnyl phosphokinase, galactose dehydrogenase, galactose-6-phosphate dehydrogenase, glutamate dehydrogenase [nicotinamide adenine dinucleotide], glutamate dehydrogenase [nicotinamide adenine dinucleotide phosphate], α-glycerophosphate dehydrogenase, and xanthine dehydrogenase). An average group cluster analysis was carried out on the basis of estimates of dissimilarity coefficients. This analysis distinguished among the strains tested, was of some help in identification, and may also be useful in epidemiological investigations.


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