1887

Abstract

Thirteen strains representing three named species, , and , were examined for the presence of 30 enzymes by horizontal starch gel electrophoresis. A total of 29 enzymes were demonstrated. Twelve of these enzymes have not been reported previously in acholeplasmas (alcohol dehydrogenase, aldolase, alkaline phosphatase, arginase, arginine deiminase, carbarnyl phosphokinase, galactose dehydrogenase, galactose-6-phosphate dehydrogenase, glutamate dehydrogenase [nicotinamide adenine dinucleotide], glutamate dehydrogenase [nicotinamide adenine dinucleotide phosphate], α-glycerophosphate dehydrogenase, and xanthine dehydrogenase). An average group cluster analysis was carried out on the basis of estimates of dissimilarity coefficients. This analysis distinguished among the strains tested, was of some help in identification, and may also be useful in epidemiological investigations.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-33-2-166
1983-04-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/33/2/ijs-33-2-166.html?itemId=/content/journal/ijsem/10.1099/00207713-33-2-166&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittler R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol 20:35–58
    [Google Scholar]
  2. Ashton G. C., Braden A. W.H. 1961; Serum β-globulin polymorphism in mice. J. Exp. Biol. Med. Sci 14:248–253
    [Google Scholar]
  3. Baur E. W., Schorr R. T. 1969; Genetic polymorphism of tetrazolium oxidase in dogs. Science 166:1524–1525
    [Google Scholar]
  4. Black F. T. 1973; Modifications of the growth inhibition test and its application to human T-mycoplasmas. Appl. Microbiol 25:528–533
    [Google Scholar]
  5. Brewer G. J. 1970; An introduction to isoenzyme techniques. Academic Press, Inc.; London:
    [Google Scholar]
  6. Carter R. 1973; Enzyme variation in Plasmodium berghei and Plasmodium vinckei . Parasitology 66:297–307
    [Google Scholar]
  7. Clayton J. W., Tretiak D. N. 1972; Amine citrate buffers for pH control in starch gel electrophoresis. J. Fish. Res. Board Can 29:1169–1172
    [Google Scholar]
  8. Ernø H., Salih M. M. 1980; The growth precipitation test as a diagnostic method for differentiation of Myco-plasma and Acholeplasma species. Acta Vet. Scand 21:469–481
    [Google Scholar]
  9. Fildes R. A., Harris H. 1966; Genetically determined variation of adenylate kinase in man. Nature London: 209261–263
    [Google Scholar]
  10. Frydenberg O., Simonsen V. 1973; Genetics of Zoarces population V. Amount of protein polymorphism and degree of genic heterozygosity. Hereditas 75:221–232
    [Google Scholar]
  11. Giblett E. R. 1969; Genetic markers in human blood. Blackwell Scientific Publications; Oxford:
    [Google Scholar]
  12. Godfrey D. G., Kilgour V. 1976; Enzyme electrophoresis in characterizing the causative organism of Gambian trypanosomiasis. Trans. R. Soc. Trop. Med. Hyg 70:219–224
    [Google Scholar]
  13. Harris H., Hopkinson D. A. 1976; Handbook of enzyme electrophoresis in human genetics. , 1. North-Holland Publishing Co.; Amsterdam:
    [Google Scholar]
  14. Heitmann J., Kirchhoff H. 1978; Acholeplasma species differentiation with the growth precipitation test. Int. J. Syst. Bacteriol 28:96–98
    [Google Scholar]
  15. Hjorth J. P. 1971; Genetics of Zoarces populations. I.. Three loci determining the phosphoglucomutase isoen-zymes in brain tissue. Hereditas 69:233–242
    [Google Scholar]
  16. Kilgour V., Godfrey D. G. 1973; Species-characteristic isoenzymes of two aminotransferases in trypanosomes. Nature (London) New Biol 244:69–70
    [Google Scholar]
  17. Lanham S. M., Lemcke R. M., Scott C. M., Grendon J. M. 1980; Isoenzymes in two species of Acholeplasma. 3 . Gen. Microbiol 117:19–31
    [Google Scholar]
  18. Lee G. Y., Kenny G. E. 1980; Electrophoretic and immunologic heterogeneity of the superoxide dismutases in the Acholeplasmataceae, p. 39. Third Conference of the International Organization for Mycoplasmology; Custer S. D.:
    [Google Scholar]
  19. Lemcke R. M., Lanham S. M., Scott C. M. 1978; Isoenzymes in two species of Acholeplasma, p. 175. Second Conference of the International Organization for Mycoplasmology; Freiburg, West Germany:
    [Google Scholar]
  20. Lynn R. J., Guldici P. A., Hollenweguer E. J., Smith P. F. 1980; Humoral immune response of rabbits to acholeplasmal lipoglycans. Infect. Immun 29:926–933
    [Google Scholar]
  21. Neimark H. C. 1974; Implications of the phylogenetic relationship between Acholeplasma and lactic acid bacteria. Colloq. Inst. Natl. Santi Rech. Med 33:71–78
    [Google Scholar]
  22. Neimark H. C. 1974; Implications of the phylogenetic relationship between Acholeplasma and lactic acid bacteria. Collog. Inst. Natl. Santd Rech. Med 33:71–78
    [Google Scholar]
  23. Neimark H. C. 1979; Phylogenetic relationships between mycoplasmas and other prokaryotes, p. 43–61 Barile M. F., Razin S. The mycoplasmas, vol. 1.Cell biology Academic Press, Inc.; New York:
    [Google Scholar]
  24. Nelson R. L., Povey S., Hopkinson D. A., Harris H. 1977; Detection after electrophoresis of enzymes involved in ammonia metabolism using L-glutamate dehydrogenase as a linking enzyme. Biochem. Genet 15:1023–1035
    [Google Scholar]
  25. O’Brien S. J., Simonsen J. M., Grabowski M. W., Barile M. F. 1981; Analysis of multiple isoenzyme expression among twenty-two species of Mycoplasma and Acholeplasma . J. Bacteriol 146:222–232
    [Google Scholar]
  26. Rosendal S., Black F. T. 1972; Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta Pathol. Microbiol. Scand. Sect B80:615–622
    [Google Scholar]
  27. Rosendal S., Valdivieso-Garcia A. 1981; Enumeration of mycoplasmas after acridine orange staining. Appl. Environ. Microbiol 41:1000–1002
    [Google Scholar]
  28. Schwartz M. K., Nisselbaum J. S., Bodansky O. 1963; Procedure for staining zones of activity of glutamic oxaloacetic transaminase following electrophoresis with starch gel. Am. J. Clin. Pathol 40:103–106
    [Google Scholar]
  29. Selander R. K., Smith M. H., Yang S. Y., Johnson W. E. 1971; Biochemical polymorphism and systematics in the genus Peromyscus . Variation in the old field mouse Peromyscus polionotus. Stud. Genet 6:49–90
    [Google Scholar]
  30. Shaw C. R. 1968; Starch gel recipe. s. Isosyme Bull 1:29–30
    [Google Scholar]
  31. Simonsen V., Frydenberg O. 1972; Genetics of Zoarces population. II. Three loci determining esterase isoenzymes in eye and brain tissue. Hereditas 70:235–242
    [Google Scholar]
  32. Simonsen V., Salih M. M. 1982; Visualization of carbomate kinase in Acholeplasma species. Isosyme Bull 15:137–138
    [Google Scholar]
  33. Smith I. 1968; Chromatographic and electrophoretic techniques. , 2. 2 Zone electrophoresis William Heinemann; London:
    [Google Scholar]
  34. Sneath P. H. A., Sokal R. R. 1973; Numerical taxonomy. W. H. Freeman; San Francisco:
    [Google Scholar]
  35. Spencer N., Harris H., Hopkinson D. A. 1968; Adenosine deaminase polymorphism in man. Ann. Hum. Genet 32:9–14
    [Google Scholar]
  36. Spencer N., Hopkinson D. A., Harris H. 1964; Phosphoglucomutase polymorphism in man. Nature London: 204742–745
    [Google Scholar]
  37. Weickmann J. L., Fahrney D. E. 1977; Arginine deiminase from Mycoplasma arthritidis. J. Biol. Chem 252:2615–2620
    [Google Scholar]
  38. Wellendorf H., Simonsen V. 1978; A chemotaxonomic study in Picea with isoenzymes in the seed endosperm, p. 182–193 Conference on the Biochemistry and Genetics of Forest Trees, Umeå; Sweden:
    [Google Scholar]
  39. Yndgaard C. F. 1972; Genetically determined electrophoretic variants of phosphoglucose isomerase and 6-phos-phogluconate dehydrogenase in Zoarces viviparus L. Hereditas. 71:151–154
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-33-2-166
Loading
/content/journal/ijsem/10.1099/00207713-33-2-166
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error