Whole-cell fatty acid analyses of 79 strains demonstrated that all 79 strains were closely related and were distinct from other pseudomonads whose fatty acid profiles were reported previously. Outer membranes were isolated from seven representative strains, and the protein patterns were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Four major outer membrane proteins were found in each of the strains but not in the outer membranes of 8 other strains or 10 other fish pathogens. The outer membranes of the seven strains studied were immunologically cross-reactive. We suggest that outer membrane protein patterns may be a useful taxonomic tool.


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