1887

Abstract

The physiology, metabolism, nutrition, and biochemical characteristics of (Glage) Eberson were studied in detail to determine the taxonomic status of this organism. . is a gram-positive, nonmotile, nonsporulating, short, rod-shaped bacterium which produces acid but not gas from a variety of carbohydrates. A number of amino acids were tested and did not appear to serve as sources of energy for growth. . is urease and catalase negative, does not reduce nitrates, and does not produce indole. Wide zones of β-hemolysis on blood agar, acid coagulation of litmus milk, and digestion of the clot are characteristic. Growth is comparable under aerobic and strictly anaerobic conditions. Metabolism is strictly fermentative. Glucose is fermented in CO-containing media to succinate, acetate, formate, and lactate; no propionic acid is produced. In identical media without CO, lactate is the major product, and only small amounts of acetate, succinate, and formate are produced. Hemin is stimulatory or required for growth. CO/HCO and inositol seem to be obligatory growth factors. Certain peptides appear to relieve the requirement for inositol. All strains require riboflavin and nicotinic acid, and most require adenine and uracil for optimal growth. Characteristic cell wall sugar components are rhamnose and glucose, and the major diamino acid of peptidoglycan is lysine. Cells contain a type cytochrome. Based on these data, we propose that . be transferred to the genus as (Glage) comb. nov.

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1982-10-01
2022-07-03
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