1887

Abstract

Seventeen representative strains of and related taxa were analyzed for antigenic relationships by rocket Immunoelectrophoresis. A good correlation was found between data from this form of analysis and previously reported numerical phenetic and deoxyribonucleic acid reassociation data. and strains formed distinct clusters in a dendrogram produced by average linkage analyses for dissimilarity matrices. , and an unidentified strain formed single-member clusters, whereas was found to be heterogeneous. In contrast, . and . were found to be distinct species. The discrepancies between deoxyribonucleic acid pairing and rocket Immunoelectrophoresis results can be explained on the basis of suboptimal antigen or antiserum preparations.

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1982-07-01
2024-04-25
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References

  1. Chaparas S. D., Baer H. 1963; The immunology and chemistry of tuberculin. II. Chromatography with Sephadex of the nondialyzable tuberculin-active constituents of BCG culture filtrate. Am. Rev. Respir. Dis 89:41–48
    [Google Scholar]
  2. Chaparas S. D., Brown T. M., Hyman I. S. 1978; Antigenic relationships among species of Mycobacterium studied by fused rocket immunoelectrophoresis. Int. J. Syst. Bacteriol 28:547–560
    [Google Scholar]
  3. Chaparas S. D., Brown T. M., Hyman I. S. 1978; Antigenic relationship of various mycobacterial species with Mycobacterium tuberculosis . Am. Rev. Respir. Dis 117:1091–1097
    [Google Scholar]
  4. Chaparas S. D., Maloney C. J. 1978; An analysis of cross reactions among mycobacteria by in vivo and in vitro assays of cellular hypersensitivity. Am. Rev. Respir. Dis 117:897–902
    [Google Scholar]
  5. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria. J. Gen. Microbiol 64:33–80
    [Google Scholar]
  6. Goodfellow M., Alderson G. 1977; The actinomycete genus Rhodococcus: a home for the ‘rhodochrous’ complex . J. Gen. Microbiol 100:99–122
    [Google Scholar]
  7. Hyman I. S., Chaparas S. D. 1977; A comparative study of the ‘rhodochrous’ complex and related taxa by delayed type of skin reactions on guinea pigs and by polyacrylamide gel electrophoresis. J. Gen. Microbiol 100:363–371
    [Google Scholar]
  8. Magnusson M. 1961; Specificity of mycobacterial sensitins. I. Studies in guinea pigs with purified “tuberculin” prepared from mammalian and avian tubercle bacilli, Mycobacterium balnei and other acid fast bacilli. Am. Rev. Respir. Dis 83:57–65
    [Google Scholar]
  9. Mordarski M., Goodfellow M., Kaszen I., Tkacz A., Pulverer G., Schaal K. P. 1980; Deoxyribonucleic acid reassociation in the classification of the genus Rhodococcus Zopf 1891 (Approved Lists, 1980). Int. J. Syst. Bacteriol 30:521–527
    [Google Scholar]
  10. Mordarski M., Goodfellow M., Szyba K., Pulverer G., Tkacz A. 1977; Classification of the “rhodochrous” complex and allied taxa based upon deoxyribonucleic acid reassociation. Int. J. Syst. Bacteriol 27:31–37
    [Google Scholar]
  11. Mordarski M., Szyba K., Pulverer G., Goodfellow M. 1976; Deoxyribonucleic acid reassociation in the classification of the ‘rhodochrous’ complex and allied taxa. J. Gen. Microbiol 94:235–245
    [Google Scholar]
  12. Svendsen P. J. 1973; Fused rocket Immunoelectrophoresis. Scand. J. Immunol 2: Suppl. 1 69–70
    [Google Scholar]
  13. Tsukamura M., Mizuno S., Tsukamura S., Tsukamura J. 1979; Comprehensive numerical classification of 369 strains of Mycobacterium, Rhodococcus, and Nocardia. Int. J. Syst. Bacteriol 29:110–129
    [Google Scholar]
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