1887

Abstract

A bacterium isolated from desert tortoises fits the major criteria for inclusion in the genus (gram-negative, nonmotile, pleomorphic rods that are facultatively anaerobic, fermentative in their attack on various carbohydrates, and catalase, nitratase, and oxidase positive). Tests performed for identifying this organism to the species level produced results that fit none of the currently accepted species of . This organism did not hydrolyze urea but did produce indole from tryptophane, hydrolyze -nitrophenyl-β--galactopyranoside, and lyse sheep erythrocytes in agar. It fermented a variety of carbohydrates, producing acetic, lactic, propionic, and succinic acids as a result of glucose fermentation. All strains were highly susceptible to ampicillin (≤0.5 μg/ml), and most strains were susceptible to penicillin (≤1 μg/ml); the susceptibilities to tetracycline, streptomycin, and sulfonamides were variable. Serological tests revealed antigenic heterogeneity and suggested the presence of heat-extractable surface antigens in some strains. Mice succumbed to intranasal and intraperitoneal inoculations of large numbers of some of the strains. The guanine-plus-cytosine content of the deoxyribonucleic acid was between 46 and 47 mol%. The Analytab Products API 20E bacterial identification system could not identify some strains of this organism. Other strains were identified as or at probability levels ranging from 1 :69 to 1 :>10. Species status is proposed for these isolates under the name sp. nov. The type strain of this species is strain UCD 90-23-79n (= ATCC 33688).

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1982-04-01
2022-10-07
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References

  1. Aarsleff B., Biberstein E. L., Shreeve B. J., Thompson D. A. 1970; A study of untypable strains of Pasteurella haemolytica . J. Comp. Pathol 80:493–498
    [Google Scholar]
  2. Allen S. D., Siders J. A. 1980; Procedures for isolation and characterization of anaerobic bacteria. 397–417 Lennette E. H., Balows A., Hausler W. J. Jr., Truant J. P. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  3. Biberstein E. L. 1978; Biotyping and serotyping of Pasteurella haemolytica . 253–269 Bergan T. Methods in microbiology 10 Academic Press, Inc.; New York:
    [Google Scholar]
  4. Biberstein E. L. 1978; The pasteurelloses. 495–514 Steele J. Handbook of zoonoses CRC Press; Boca Raton, Fla:
    [Google Scholar]
  5. Carter G. R. 1979; Diagnostic procedures in veterinary bacteriology and mycology. , 3. Charles C Thomas, Publisher, Springfield;111
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965; Manual for the identification of medical bacteria. Cambridge University Press; Cambridge:
    [Google Scholar]
  7. Difco Laboratories 1969; Difco manual of dehydrated culture media and reagents for microbiological and clinical laboratory procedures. , 9. Difco Laboratories; Detroit, Mich:
  8. Edwards P. R., Ewing W. H. 1972; Identification of the Enterobacteriaceae . , 2. Burgess Publishing Co.; Minneapolis, Minn:
    [Google Scholar]
  9. ElweU L. P., DeGroaf J., Seibert D., and S. Falkow. 1975; Plasmid-linked ampicillin resistance in Haemophilus inftuenzae type b. Infect. Immun 12:404–410
    [Google Scholar]
  10. Flossmann K. O., Pohrmann B., Feist H., Erler W. 1979; Desoxyribonukleinsauren aus Pasteurella multocida und Pasteurella haemolytica . Arch. Exp. Vet. Med 33:393–402
    [Google Scholar]
  11. Frederiksen W. 1973; Pasteurella taxonomy and nomenclature. Contrib. Microbiol. Immunol 2:170–176
    [Google Scholar]
  12. Frye F. L. 1977; Bacterial and fungal diseases of captive reptiles. 787–791 Kirk R. W. Current veterinary therapy 6 The W. B. Saunders Co.; Philadelphia:
    [Google Scholar]
  13. Henriksen P., Graham D. L. 1972; Diagnosis and treatment of disease in the turtle. Iowa State Univ. Vet 34:29–32
    [Google Scholar]
  14. Holdeman L. V., Moore W. E. C. 1975; Anaerobe laboratory manual. , 3. Virginia Polytechnic Institute and State University Anaerobe Laboratory; Blacksburg:
    [Google Scholar]
  15. Mainster M. E., Lynd F. T., Cragg P. C., Karger J. 1973; Treatment of multiple cases of P. multocida and staphylococcal pneumonia in Alligator mississippiensis on a herd basis. 34–36Annual Proceedings of the American Association of Zoo VeterinariansAAZV, Houston
    [Google Scholar]
  16. Mayer H., Frank W. 1974; Bacteriological studies on reptiles and amphibians. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe 229:470–481
    [Google Scholar]
  17. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of the blood. J. Hyg 38:732–749
    [Google Scholar]
  18. Mraz D. 1970; Indolbildende Variante des Actinobacillus haemolyticus . Zentralbl. Bakteriol. Parasitenkd. Infektionskr 213:93–102
    [Google Scholar]
  19. Paik G. 1980; Reagents, stains, and miscellaneous test procedures. 1000–1024 Lennette E. H., Balows A., Hausler W. J. Jr., Truant J. P. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  20. Phillips J. E. 1974; Actinobacillus. 373–377 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  21. Reed L. J., Muench H. 1938; A simple method for estimating fifty percent endpoints. Am. J. Hyg 27:493–497
    [Google Scholar]
  22. Reichenbach-Klinke H., Elkan E. 1966; The principal diseases of lower vertebrates. Academic Press, Inc.; New York:
    [Google Scholar]
  23. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCI. J. Mol. Biol 4:430–433
    [Google Scholar]
  24. Schubert R. H. W. 1974; Aeromonas. 345–348 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  25. Shreeve B. J., Thompson D. A. 1970; Studies on the carriage of Pasteurella hemolytica in lambs. J. Comp. Pathol 80:107–112
    [Google Scholar]
  26. Smith G. R. 1961; The characteristics of two types of Pasteurella haemolytica associated with diflFerent pathological conditions in sheep. J. Pathol. Bacteriol 81:431–440
    [Google Scholar]
  27. Smith J. E. 1974; Pasteurella. 370–373 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  28. Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. 1972; API system: a multitube method for identification of Enterobacteriaceae . Appl. Microbiol 24:449–452
    [Google Scholar]
  29. Snipes K. P., Biberstein E. L., Fowler M. E. 1980; A Pasteurella sp. associated with respiratory disease in captive desert tortoises. J. Am. Vet. Med. Assoc 177:804–807
    [Google Scholar]
  30. Stamp J. T., Watt A. A., Thomlinson J. R. 1955; Pasteurella hemolytica septicaemia of lambs. J. Comp. Pathol. Ther 65:183–196
    [Google Scholar]
  31. Wallach J. D. 1969; Medical care of reptiles. J. Am. Vet. Med. Assoc 155:1017–1034
    [Google Scholar]
  32. Washington J. A. II, Sutter V. L. 1980; Dilution susceptibility test: agar and macro-broth dilution procedures. 453–458 Lennette E. H., Balows A., Hausler W. J. Jr., Truant J. P. Manual of clinical microbiology, 3. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
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