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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 32, Issue 1

Base Compositions and Homologies of Deoxyribonucleic Acids of Corynebacteria Isolated from Human Leprosy Lesions and of Related Microorganisms Free

Abstract

The deoxyribonucleic acids (DNAs) of 25 strains of leprosy-derived corynebacteria (LDC)—non-acid-fast, gram-positive bacteria independently isolated from human leprosy lesions and propagated in axenic culture—were purified and analyzed. The guanine plus cytosine content, by buoyant density determination, was 54 to 59 mol% for most LDC strains, a range that corresponds to that (50 to 60 mol%) of corynebacteria which multiply in animal cells. These values were checked by chromatographic analyses of acid digests of the DNAs. The taxonomic position of the LDC as determined by DNA base composition was confirmed by the results of the corynomycolic acid determinations of the walls of the LDC. The results of the hybridization of the DNAs from the LDC strains suggest the occurrence of two high-homology groups, in which most of the strains were accommodated. In contrast, little homology was observed between the DNAs of the LDC and the reference corynebacteria employed. From these data, it can be inferred that the LDC represent a homogeneous and unique cluster of organisms within the genus , more specifically within the group of corynebacteria pathogenic for humans.

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Copyright © 1982 International Union of Microbiological Societies
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1982-01-01
2023-03-30
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References

  1. Baes I., Weis Bentzon M. 1978; DNA hybridization between different species of mycobacteria. Acta Pathol. Microbiol. Scand. Sect. B 86:71–76
     [Google Scholar]
  2. Beaman B. L., Kwang-Shin K., Lanéelle M. A., Barksdale L. 1974; Chemical characterization of organisms isolated from leprosy patients. J. Bacteriol 117:1320–1329
     [Google Scholar]
  3. Bernardi G. 1971; Chromatography of nucleic acids on hydroxyapatite columns. Methods Enzymol 21:95–139
     [Google Scholar]
  4. Bouisset L., Brueillaud J., Michel G. 1963; Etude de l’ADN chez les actinomycétales: Comparaison entre les valeurs du rapport A+T/G+C et les caractères bactériologiques de Corynebacterium . Ann. Inst. Pasteur Paris 104:756–770
     [Google Scholar]
  5. Bousfield I. J. 1972; A taxonomic study of some coryne-form bacteria. J. Gen. Microbiol 71:441–455
     [Google Scholar]
  6. Bradley S. G. 1973; Relationships among mycobacteria and nocardiae based upon DNA reassociation. J. Bacteriol 113:645–651
     [Google Scholar]
  7. Bradley S. G., Brownell G. H., Clarck J. 1973; Genetic homologies among nocardiae and other actinomycetes. Can. J. Microbiol 19:1007–1014
     [Google Scholar]
  8. Cocito C. 1969; Metabolism of macromolecules in bacteria treated with virginiamycin. J. Gen. Microbiol 57:179–194
     [Google Scholar]
  9. Crombach W. H. J. 1978; DNA base ratios and DNA hybridization studies of coryneform bacteria, mycobacteria and nocardiae. 161–179 Bousfied I. J., Gallely A. G. Coryneform bacteria Academic Press, Inc.; New York:
     [Google Scholar]
  10. Delville J. 1972; Considérations sur la multiplication de M. leprae et essais de culture. Acta Leprolog 48/49:105–111
     [Google Scholar]
  11. Delville J., Pichel A. M. 1975; L’agent étiologique de la lèpre est-il invariablement acido-alcoolo resistant au Ziehl-Neelsen? Problèmes soulevés par les isolements à partir de lésions lépreuses de germes non acido-alcoolo résistants. Acta Leprolog 59/60:83–91
     [Google Scholar]
  12. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol 12:829–842
     [Google Scholar]
  13. Gross W. M., Wayne L. G. 1970; Nucleic acid homology in the genus Mycobacterium . J. Bacteriol 104:630–634
     [Google Scholar]
  14. Janczura E., Leyh-Bouille M., Ghuysen J. M., Cocito C. 1981; Structure of the peptidoglycans obtained from a group of corynebacteria. J. Bacteriol 145:775–779
     [Google Scholar]
  15. Laub R., Delville J., Cocito C. 1978; Immunological relatedness of ribosomes from mycobacteria, nocardiae and corynebacteria, and microorganisms in leprosy lesions. Infect. Immun 22:540–547
     [Google Scholar]
  16. Lind A., Ridell M. 1976; Serological relationships between Nocardia, Mycobacterium, Corynebacterium and the “rhodochrous” taxon. 220–235 Goodfellow M., Brownell G. H., Serrano J. A. The biology of the nocardiae Academic Press, Inc.; New York:
     [Google Scholar]
  17. Mackey J. K., Brackmann K. H., Green M. R., Green M. 1977; Preparation and characterization of highly radioactive in vitro labeled adenovirus DNA and DNA restriction fragments. Biochemistry 16:4478–4483
     [Google Scholar]
  18. Mandel M., Schildkraut C. L., Marmur J. 1968; Use of the CsCl density gradient analysis for determining the guanine plus cytosine content of DNA. Methods Enzymol 12:184–195
     [Google Scholar]
  19. Markov M., Ivanov I. G. 1974; Hydroxyapatite column chromatography in procedures for isolation of purified DNA. Anal. Biochem 59:555–563
     [Google Scholar]
  20. Mizuguchi Y., Tokunaga T. 1970; Method for isolation of deoxyribonucleic acid from mycobacteria. J. Bacteriol 104:1020–1021
     [Google Scholar]
  21. Ridell M. 1977; Studies on corynebacterial precipitinogens common to mycobacteria, nocardiae and rhodochrous. Int. Arch. Allergy Appl. Immunol 55:847–875
     [Google Scholar]
  22. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by “nick translation” with DNA polymerase I. J. Mol. Biol 113:237–251
     [Google Scholar]
  23. Skerman V. D. B., McGowan V., Sneath P. H. A. (ed.). 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol 30:225–420
     [Google Scholar]
  24. Tewfik E. M., Bradley S. G. 1967; Characterization of deoxyribonucleic acids from streptomycetes and nocardiae. J. Bacteriol 94:1994–2000
     [Google Scholar]
  25. Warnaar S. O., Cohen J. A. 1966; A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem. Biophys. Res. Commun 24:554–558
     [Google Scholar]
  26. Wayne L. G., Gross W. M. 1968; Isolation of deoxyribonucleic acid from mycobacteria. J. Bacteriol 95:1481–1482
     [Google Scholar]
  27. Wayne L. G., Gross W. M. 1968; Base composition of DNA isolated from mycobacteria. J. Bacteriol 96:1915–1919
     [Google Scholar]
  28. Wyatt G. R. 1955; Separation of nucleic acid components by chromatography on filter paper. 243–265 Chargaff E, Davidson J. N. The nucleic acids, chemistry and biology 1 Academic Press, Inc.; New York:
     [Google Scholar]
  29. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. 3. DNA base composition of coryneform bacteria. J. Gen. Appl. Microbiol 16:215–224
     [Google Scholar]
  30. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. J. Gen. Appl. Microbiol 18:417–431
     [Google Scholar]
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Base Compositions and Homologies of Deoxyribonucleic Acids of Corynebacteria Isolated from Human Leprosy Lesions and of Related Microorganisms
Int J Syst Evol Microbiol 32, 70 (1982); https://doi.org/10.1099/00207713-32-1-70
/content/journal/ijsem/10.1099/00207713-32-1-70
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