1887

Abstract

The deoxyribonucleic acid (DNA) relatedness between a newly described, homofermentative species, , and a broad spectrum of homofermentative lactobacilli was determined. A low range of DNA reassociation levels (5 to 33%) was observed between and those species () with which it shared one or more phenotypic characteristics (such as the production of mainly -(+)-lactic acid from glucose, hydrolysis of starch, the sugar fermentation pattern) or which had a guanine plus cytosine content of 45 mol%. A lack of DNA relatedness (6 to 20% reassociation) was noted between and 10 other homofermentative lactobacilli. A DNA reassociation of 90 to 98% was measured among the tested strains of . Purified cell walls of contained alanine, aspartic acid, glutamic acid, and lysine, but no diaminopimelic acid. The low DNA relatedness indicated that the strains were not highly related genetically to the established homofermentative species and, hence, that they represent strains of a distinct genospecies.

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1982-01-01
2022-12-09
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References

  1. Brenner D. J. 1975; Deoxyribonucleic acid reassociation in the taxonomy of enteric bacteria. Int. J. Syst. Bacteriol 23:298–307
    [Google Scholar]
  2. Carr J. G., Davies P. A., Dettaglio F., Vescovo M. 1977; The relationship between Lactobacillus mali from cider and Lactobacillus yamanashiensis from wine. J. Appl. Bacteriol 42:219–228
    [Google Scholar]
  3. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing clostridia. J. Gen. Microbiol 67:33–46
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurements of DNA hybridization from renaturation rates. Eur. J. Biochem 12:133–142
    [Google Scholar]
  5. de Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J. Appl. Bacteriol 23:130–135
    [Google Scholar]
  6. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun 23:641–646
    [Google Scholar]
  7. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem 12:143–153
    [Google Scholar]
  8. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the gua-nine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  9. Markov G. G., Ivanov I. G. 1974; Hydroxyapatite column chromatography procedure for isolation of purified DNA. Anal. Biochem 59:555–563
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  11. Myers W. F., Wiseman C. L. Jr 1980; Genetic relatedness among the typhus group of rickettsiae. Int. J. Syst. Bacteriol 30:143–150
    [Google Scholar]
  12. Nakamura L. K. 1981; Lactobacillus amylovorus a new starch-hydrolyzing species from cattle wastecorn fermentations. Int. J. Syst. Bacteriol 31: 00-00
    [Google Scholar]
  13. Nakamura L. K. 1981; Lactobacillus amylophilus. In validation of new names and new combinations previously effectively published outside the USB. List no. 6. Int. J. Syst. Bacteriol 31: 00-00
    [Google Scholar]
  14. Nakamura L. K., Crowell C. D. 1979; Lactobacillus amylophilus a new starch-hydrolyzing species from swine waste-corn fermentation. Dev. Ind. Microbiol 20:532–540
    [Google Scholar]
  15. Rogosa M. 1974; Genus I. Lactobacillus . 576–593 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Company; Baltimore:
    [Google Scholar]
  16. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cells walls and their taxonomic implications. Bacteriol. Rev 36:407–477
    [Google Scholar]
  17. Seidler R. J., Mandel M. 1971; Quantitative aspects of deoxyribonucleic acid renaturation; base composition, state of chromosome replication, and polynucleotide homologies. J. Bacteriol 106:608–614
    [Google Scholar]
  18. Sharpe M. E., Dettaglio F. 1977; Deoxyribonucleic acid homology in anaerobic lactobacilli and in possibly related species. Int. J. Syst. Bacteriol 27:19–21
    [Google Scholar]
  19. Skerman V. B. D., McGowan V., Sneath P. H. A. (ed). 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol 30:225–420
    [Google Scholar]
  20. Trevelyan W. E., Porter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature (London) 166:444
    [Google Scholar]
  21. Wetmur J. G., Davidson N. 1968; Kinetics of renaturation of DNA. J. Mol. Biol 31:349–370
    [Google Scholar]
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