We divided the asaccharolytic, black-pigmented strains into two groups on the basis of deoxyribonucleic acid (DNA) base ratios, DNA hybridization (S1 nuclease method) results, and direct hemagglutination. One homologous group of strains, which included the type strain of and had guanine-plus-cytosine contents of 52 to 54 mol%, contained only nonoral isolates. Another DNA homology group contained all of the strains from periodontal pockets and some nonoral isolates. These strains appeared to belong to the recently described new species Coykendall et al. strains had guanine-plus-cytosine contents of 48 to 50 mol%, showed high DNA homology values, and shared hemagglutinating activity. No DNA homology was observed between the two groups. Using a different method of analysis for DNA homology (S1 nuclease method), we confirmed the conclusion of Coykendall et al., who separated these two groups into different species, and . Two strains from infected root canals could not be placed in either of these two species. On the basis of the DNA homology results, all asaccharolytic strains were distinguished clearly from the saccharolytic, black-pigmented strains, which at present are classified in the species .


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error