1887

Abstract

Deoxyribonucleotide sequence relationships among currently recognized species (, and ) were examined by deoxyribonucleic acid (DNA) hybridization involving the hydroxyapatite batch procedures of Brenner and co-workers. The results indicated that strains from all species tested were highly related. At the stringent criterion (80°C), the relative binding of DNA to DNA was 75 ± 9%, and to DNA it was 73 ± 8%. Intraspecies binding was 93 ± 8%. Under similar conditions, the relative binding of DNA to DNA was 85 ± 9%. The various so-called species may be reconsidered as representing different subspecies belonging to a single species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-31-2-173
1981-04-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/31/2/ijs-31-2-173.html?itemId=/content/journal/ijsem/10.1099/00207713-31-2-173&mimeType=html&fmt=ahah

References

  1. Brenner D. J., Fanning G. R., Rake A., Johnson K. E. 1969; A batch procedure for thermal elution of DNA from hydroxyapatite. Anal. Biochem 28:447–459
    [Google Scholar]
  2. Kloos W. E. 1980; Natural populations of the genus Staphylococcus. Annu. Rev. Microbiol 34:559–592
    [Google Scholar]
  3. Kloos W. E., Dobrogosz W. J., Ezzell J. W., Kimbro B. R., Manclark C. R. 1978; DNA-DNA hy-bridization, plasmids, and genetic exchange in the genus Bordetella. p 70–80 In Manclark C. R., Hill J. C. ed International Symposium on Pertussis, Department of Health, Education and Welfare publication no. (NIH) 79-1830. U.S. Government Printing Office; Washington, D.C:
    [Google Scholar]
  4. Kloos W. E., Wolfshohl J. F. 1979; Evidence for deoxyribonucleotide sequence divergence between staphylococci living on human and other primate skin. Curr. Microbiol 3:167–172
    [Google Scholar]
  5. Linnemann C. C. 1978; Host-parasite interactions in pertussis. p 3–18 In Manclark C. R., Hill J. C. ed International Symposium on Pertussis, Department of Health, Education and Welfare publication no. (NIH) 79-1830. U.S. Government Printing Office; Washington, D.C:
    [Google Scholar]
  6. Muse K., Findley D., Allen L., Collier A. M. 1978; In vitro model of Bordetella pertussis infection: pathogenic and microbicidal interactions. p 41–50 In Manclark C. R., Hill J. C. ed International Symposium on Pertussis, Department of Health, Education and Welfare publication no. (NIH) 79-1830. U.S. Government Printing Office; Washington, D.C:
    [Google Scholar]
  7. Pittman M. 1970; Bordetella pertussis-bacterial and host factors in the pathogenesis and prevention of whooping cough. p 239–270 In Mudd S. ed Infectious agents and host reactions. The W. B. Saunders Co; Philadelphia:
    [Google Scholar]
  8. Pittman M. 1974; Genus Bordetella Moreno-Lopez 1952. p 282–283 In Buchanan R. E., Gibbons N. E. ed Bergey’s manual of determinative bacteriology. , 8th ed.. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  9. Ross R. F., Duncan J. R., Switzer W. P. 1963; Turbinate atrophy in swine produced by pure cultures of Bordetella bronchiseptica. Vet. Med 58:566–570
    [Google Scholar]
  10. Skerman V. B. D., McGowan V., Sneath P. H. A. ed 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol 30266
    [Google Scholar]
  11. Stainer D. W., Scholte M. J. 1971; A simple chemically defined medium for the production of phase I Bordetella pertussis. J. Gen. Microbiol 63:211–220
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-31-2-173
Loading
/content/journal/ijsem/10.1099/00207713-31-2-173
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error