1887

Abstract

An immunological study of nine strains of pure-cultured members of the order isolated from symbiotic root nodules was undertaken to establish the taxonomic relationships of these organisms. Based on the data from a simple double-diffusion (Ouchterlony) precipitation procedure, these isolates, which tentatively have been classified in the genus (family ), could be divided into two major groups. The first group, designated serotype I, included all of the strains isolated from , and host plants. The second group, serotype II, included strains isolated from host plants. No ambiguous cross-reactions with unrelated members of the were observed. Crushed nodule suspensions cross-reacted in the same manner as pure-cultured frankiae, suggesting that the technique could be important in screening for novel, naturally occurring strains. A comparative study in which an immunofluorescence procedure was used failed to show reliable correlations among the strains tested. We concluded that the immunodiffusion technique was the better of the two methods for strain characterization.

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1981-04-01
2022-10-01
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