1887

Abstract

I assessed the genetic relatedness of 45 strains of heterocyst-forming cyanobacteria assigned to eight genera by Rippka et al. and of 19 undescribed strains of the same group by in vitro reassociation of radioiodinated deoxyribonucleic acids. The members of the genera , and formed discrete clusters (intrageneric values of relative binding, more than 55%) and showed intergeneric relatedness of less than 40%, results consistent with the classification proposed by Rippka et al. The genus was heterogeneous; four strains previously assigned to appeared to belong to . The genus comprised four clusters with various degrees of internal homogeneity and two strains which showed low relatedness to any others. The general relatedness (i.e., relative binding) of heterocyst formers to various non-heterocystous cyanobacteria belonging to groups II and III (both unicellular and filamentous) was on the order of 10 to 20%.

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1981-04-01
2024-04-18
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References

  1. Brenner D. J., Fanning G. R., Rake A., Johnson K. E. 1969; A batch procedure for the thermal elution of DNA from hydroxyapatite. Anal. Biochem 28:447–459
    [Google Scholar]
  2. Herdman M., Janvier M., Rippka R., Stanier R. Y. 1979; Genome size of cyanobacteria. J. Gen. Microbiol 111:73–85
    [Google Scholar]
  3. Herdman M., Janvier M., Waterbury J. B., Rippka R., Stanier R. Y., Mandel M. 1979; Deoxy-ribonucleic acid base composition of cyanobacteria. J. Gen. Microbiol 111:63–71
    [Google Scholar]
  4. Kelly M. L., Cowie D. B. 1972; DNA-DNA hybridization studies of blue-green algae. Carnegie Inst. Washington Yearb 71:276–281
    [Google Scholar]
  5. Lachance M. A. 1980; Simple method for determination of deoxyribonucleic acid relatedness by thermal elution on hydroxyapatite microcolumns. Int. J. Syst. Bacteriol 30:433–436
    [Google Scholar]
  6. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  7. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 5:109–118
    [Google Scholar]
  8. Orosz J. M., Wetmur J. G. 1974; In vitro iodination of DNA. Maximizing iodination while minimizing degradation; use of buoyant density shifts for DNA-DNA hybrid isolation. Biochemistry 13:5467–5473
    [Google Scholar]
  9. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. 1979; Generic assignments, strain histories and properties of pure cultures of cy-anobacteria. J. Gen. Microbiol iii:1–61
    [Google Scholar]
  10. Scherberg N. H., Refetoff S. 1974; The radioiodination of ribopolymers for use in hybridizational and molecular analyses. J. Biol. Chem 249:2143–2150
    [Google Scholar]
  11. Stam W. T., Venema G. 1977; The use of DNA-DNA hybridization for determination of the relation-ship between some blue-green algae (cyanophyceae). Acta Bot. Neerl 26:327–342
    [Google Scholar]
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