1887

Abstract

We determined the degree of binding between deoxyribonucleic acid (DNA) preparations from representative rhodococci and thymine-labeled DNAs (derived from labeled uracil) from the following six reference strains: (Approved Lists, 1980) strain N744, R. (Bergey et al. 1923) Goodfellow and Alderson 1979 (Approved Lists, 1980) strain N657, R. (Approved Lists, 1980) strain C7, R. (Approved Lists, 1980) strain N54, R. ruber (Approved Lists, 1980) strain N361, and (Hefferan 1904) Goodfellow and Alderson 1979 (Approved Lists, 1980) strain N4. Although the congruence between the DNA reassociation and earlier numerical phenetic data was good, there was evidence that some strains had been misclassified in the numerical phenetic study. In particular, strains labeled (including the type strain of ) and belonged to a single DNA homology group. The former name is thus a later, subjective synonym of the latter. The guanine plus cytosine contents of the DNAs of the rhodococci fell within the range of 65 to 73 mo1%.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-30-3-521
1980-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/30/3/ijs-30-3-521.html?itemId=/content/journal/ijsem/10.1099/00207713-30-3-521&mimeType=html&fmt=ahah

References

  1. Bisset K. A., Moore F. W. 1950; Jensenia, a new genus of the Actinomycetales. J. Gen. Microbiol 4:280
    [Google Scholar]
  2. Bousfield I. J., Goodfellow M. 1976; The “rhodochrous''complex and its relationships with allied taxa. 39–65 In Goodfellow M., Brownell G. H. B., Serrano J. A. (ed.) The biology of the nocardiae Academic Press, Inc.; London:
    [Google Scholar]
  3. Bradley S. G. 1975; Significance of nucleic acid hybridi zation to systematics of actinomycetes. Adv. Appl. Microbiol 19:59–70
    [Google Scholar]
  4. Bradley S. G., Bond J. S. 1974; Taxonomic criteria for mycobacteria and nocardiae. Adv. Appl. Microbiol 18:131–190
    [Google Scholar]
  5. Bradley S. G., Mordarski M. 1976; Association of polydeoxyribonucleotides of deoxyribonucleic acids from nocardioform bacteria. 310–366 In Goodfellow M., Brownell G. H., Serrano J. A. (ed.) The biology of the nocardiae Academic Press, Inc.; London:
    [Google Scholar]
  6. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Anal. Biochem. 1:279–285
    [Google Scholar]
  7. Brenner D. J. 1973; Deoxyribonucleic acid reassociation in the taxonomy of enteric bacteria. Int. J. Syst. Bacteriol 23:298–307
    [Google Scholar]
  8. Goodfellow M. 1971; Numerical taxonomy of some no cardioform bacteria. J. Gen. Microbiol 69:33–80
    [Google Scholar]
  9. Goodfellow M., Alderson G. 1977; The actino-mycete-genus Rhodococcus: a home for the “rhodochrous” complex. J. Gen. Microbiol 100:99–122
    [Google Scholar]
  10. Goodfellow M., Minnikin D. E. 1977; Nocardi oform bacteria. Annu. Rev. Microbiol 31:159–180
    [Google Scholar]
  11. Goodfellow M., Minnikin D. E. 1978; Numerical and chemical methods in the classification of Nocardia and related taxa. Zentralbl. Bakteriol. Parasitenkd. In-fektionskr. Hyg. Abt. 1 Suppl 6:43–51
    [Google Scholar]
  12. Goodfellow M., Mordarski M., Szyba K., Pul-verer G. 1978; Relationships among rhodococci based upon deoxyribonucleic acid reassociation. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Suppl 7:231–234
    [Google Scholar]
  13. Goodfellow M., Mordarski M., Tkacz A., Szyba K., Pulverer G. 1979; Polynucleotide sequence divergence among some coagulase-negative staphylococci. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 246:10–22
    [Google Scholar]
  14. Goodfellow M., Orlean P. A. B., Collins M. D., Alshamaony L., Minnikin D. E. 1978; Chemical and numerical taxonomy of strains received as Gordona aurantiaca.. J. Gen. Microbiol 109:57–68
    [Google Scholar]
  15. Gordon R. E. 1966; Some strains in search of a genus— Corynebacterium, Mycobacterium, Nocardia or what?. J. Gen. Microbiol 43:329–343
    [Google Scholar]
  16. Gordon R. E., Mihm J. M. 1959; A comparison of four species of mycobacteria. J. Gen. Microbiol 21:736748
    [Google Scholar]
  17. Huang P. C., Rosenberg E. 1966; Determination of DNA base composition via depurination. Anal. Bio-chem 16:107–113
    [Google Scholar]
  18. Jensen H. L. 1931; Contributions to our knowledge of the Actinomycetales. II. The definition and subdivision of the genus Actinomyces, with a preliminary account of Australian soil actinomycetes. Proc. Linn. Soc. N.S.W. 56:345–370
    [Google Scholar]
  19. Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  20. McClung N. M. 1974; Nocardiaceae Castellani and Chal mers. p 726–746 In Buchanan R. E., Gibbons N. E. (ed.) Bergey’s manual of determinative bacteriology, 8th ed. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  21. Minnikin D. E., Goodfellow M., Collins M. D. 1978; Lipid composition in the classification and identification of coryneform and related taxa. p 85–160 In Bousfield I. J., Callely A. G. (ed.) Coryneform bacteria Academic Press, Inc.; London:
    [Google Scholar]
  22. Mordarska H., Mordarski M., Pietkiewicz D. 1973; Chemical analysis of hydrolysates and cell extracts of Nocardia pellegrino.. Int. J. Syst. Bacteriol 23:274277
    [Google Scholar]
  23. Mordarski M., Goodfellow M., Szyba K., Pulverer G., Tkacz A. 1977; Classification of the “rkodoch-rous” complex and allied taxa based upon deoxyribonucleic acid reassociation. Int. J. Syst. Bacteriol 27:3137
    [Google Scholar]
  24. Mordarski M., Szyba K., Pulverer G., Good fellow M. 1976; Deoxyribonucleic acid reassociation in the classification of the “rhodochrous” complex and allied taxa. J. Gen. Microbiol 94:235–245
    [Google Scholar]
  25. Okanishi M., Gregory K. F. 1970; Methods for the determination of deoxyribonucleic acid homologies in Streptomyces.. J. Bacteriol 104:1086–1094
    [Google Scholar]
  26. Rowbotham T. J., Cross T. 1977; Rhodococcus coprophilus sp. nov., an aerobic nocardioform actino-mycete belonging to the “rhodochrous” complex. J. Gen. Microbiol 100:123–138
    [Google Scholar]
  27. Rowbotham T. J., Cross T. 1977; Ecology of Rhodococcus coprophilus and associated actinomycetes in freshwater and agricultural habitats. J. Gen. Microbiol 100:231–240
    [Google Scholar]
  28. Runyon E. H., Wayne L. G., Kubica G. P. 1974; Mycobacterium Lehmann and Neumann. p 681–701 In Buchanan R. E., Gibbons N. E. (ed.) Bergey’s manual of determinative bacteriology, 8th ed. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  29. Skerman V. B. D., McGowan V., Sneath P. H. A. (ed.) 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol 30:224–420
    [Google Scholar]
  30. Sneath P. H. A. 1976; An evaluation of numerical taxo nomic techniques in the taxonomy of Nocardia and allied taxa. p 74–101 In Goodfellow M., Brownell G. H., Serrano J. A. (ed.) The biology of the nocardiae Academic Press, Inc.; London:
    [Google Scholar]
  31. Staley T. E., Colwell R. R. 1973; Application of molecular genetics and numerical taxonomy to the classification of bacteria. Annu. Rev. Ecol. Syst 4:273–300
    [Google Scholar]
  32. Tsukamura M. 1971; Proposal of a new genus, Gordona, for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J. Gen. Microbiol 68:15–26
    [Google Scholar]
  33. Tsukamura M., Mizuno S., Tsukamura S., Tsukamura J. 1979; Comprehensive numerical classification of 369 strains of Mycobacterium, Rhodococcus, and Nocardia. . Int. J. Syst. Bacteriol 29:110–129
    [Google Scholar]
  34. Zopf W. 1891; Uber Ausscheidung von Fettfarbstoffen (Lipochromen) seitens gewisser Spaltpilze. Ber. Dtsch. Bot. Ges. 9:22–28
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-30-3-521
Loading
/content/journal/ijsem/10.1099/00207713-30-3-521
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error