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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 29, Issue 4

sp. nov. Free

Abstract

Strains of a new type of rapidly growing, scotochromogenic mycobacterium have been isolated repeatedly from sphagnum vegetation of moors in south Sweden and the Atlantic coastal area of Norway. These strains split urea and succinamide, hydrolyze Tween 80, produce acid from glucose, fructose, mannitol, rhamnose, sorbitol, and trehalose, and grow on a medium with fumarate, succinate, citrate, malonate, oxalate, propanol, or hippurate as the single carbon source. Furthermore, they possess acid phosphatase and putrescine oxidase activities, degrade salicylate, and metabolize iron. Additional properties of these strains are presented. The internal similarity of the strains, as determined by numerical taxonomy methods, as 94.97 ± 3.42%. A comparison with 21 species (clusters) of rapidly growing mycobacteria is also presented. The production of mycolic acid by these strains and their micromorphology confirm that they belong to the genus . The strains have unique lipid and immunodiffusion patterns and form special sensitins. These strains are considered as belonging to a new species of nonpathogenic, rapidly growing mycobacteria for which we propose the name . Strain Ko 2 is the type strain of ; a culture of this strain has been deposited in the American Type Culture Collection under the number 33013.

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1979-10-01
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References

  1. Bönicke R. 1961; Die Bedeutung der Acylamidasen furdie Identifizierung und Differenzierung der verschiede- nen Arten der Gattung Mycobacterium. Jahresber. Borstel 5:7–87
     [Google Scholar]
  2. Bönicke R. 1962; Identification of mycobacteria by biochemical methods. Bull. Int. Union Tuberc 32:13–86
     [Google Scholar]
  3. Bönicke R., Nolte H. 1967; Diamin-oxydasen in Mycobacterien. Zentralbl. Bakteriol. Parasitenkd In- fektionskr. Hyg. Abt. 1 Orig 202:479–487
     [Google Scholar]
  4. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int. J. Syst. Bacteriol 24:54–63
     [Google Scholar]
  5. Gordon R. E., Smith M. M. 1953; Rapidly growing, acid-fast bacteria. I. Species’ descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann. J. Bacteriol 66:41–48
     [Google Scholar]
  6. Käppler W. 1965; Zur Differenzierung von Mykobakterien mit dem Phosphatase-Test. Beitr. Klin. Tuberk 130:223–226
     [Google Scholar]
  7. Kazda J. 1977; Differenzierung von Mykobakterien mitder Jones-Mote-Raktion. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 237:90–95
     [Google Scholar]
  8. Kazda J. 1977; Die Bedeutung der Moorbiotope fur die Ökologie von Mykobakterien. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe B. 165:323–334
     [Google Scholar]
  9. Kazda J., Muller K., Irgens L. M. 1979; Cultivable mycobacteria in sphagnum vegetation of moors in South Sweden and coastal Norway. Acta Pathol. Microbiol. Scand. Sect. B 87:97–101
     [Google Scholar]
  10. Kubica G. P., Ridgon A. L. 1961; The arylsulfatase activity of acid-fast bacilli. III. Preliminary investigation of rapidly growing acid-fast bacilli. Am. Rev. Res- pir. Dis 83:737–740
     [Google Scholar]
  11. Marks J., Jenkins P. A., Schaefer W. B. 1971; Thin-layer chromatography of mycobacterial lipids as an aid to classification: technical improvements: Mycobacterium avium, M. intracellulare. Tubercle 52:219–225
     [Google Scholar]
  12. Meissner G. 1959; Untersuchungen an atypischen My kobakterien. II. Vergleichende tierexperimentelle Untersuchungen zur Frage ihrer Pathogenitat und Viru- lenz. Beitr. Klin. Tuberk. Spezifischen Tuberk. Forsch 121:365–380
     [Google Scholar]
  13. Minninkin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-cell methanolysates. J. Gen. Microbiol 88:200
     [Google Scholar]
  14. Oswald H. 1923; Die Vegetation des Hochmoores Komosse. Handbook of the Sven. Växtsoc., Sällsk, Uppsala
     [Google Scholar]
  15. Pattyn S. R., Portaels F. 1972; Identification and clinical significance of mycobacteria. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 219:114–140
     [Google Scholar]
  16. Saito H., Gordon R. E., Juhlin I., Käppler W., Kwapinski J. B. G., McDurmont C., Pattyn S. R., Runyon E. H., Stanford H. L., Tarnok I., Tasaka H., Tsukamura M., Weiszfeiler J. 1977; Cooperative numerical analysis of rapidly growing mycobacteria. The second report. Int. J. Syst. Bacteriol 27:75–85
     [Google Scholar]
  17. Sokal R. R., Sneath P. H. A. 1963; Principles of numerical taxonomy. Freeman and Co; San Francisco:
     [Google Scholar]
  18. Stanford H. L. 1973; Immunodiffusion analysis—a rational basis for the taxonomy of mycobacteria. Ann. Soc. Beige Med. Trop 53:321–330
     [Google Scholar]
  19. Szabo J., Vandra E. 1963; Mycobacterium minetti (Penso et al. 1952) bacteriological and epidemiological observation. Acta Microbiol. Acad. Sci. Hung 10:215–223
     [Google Scholar]
  20. Tsukamura M. 1965; Differentiation of mycobacteria by susceptibility to hydroxylamine and 8-azaguanine. J. Bacteriol 90:556–557
     [Google Scholar]
  21. Tsukamura M. 1965; Salicylate degradation test for differentiation of Mycobacterium fortuitum from other mycobacteria. J. Gen. Microbiol 41:309–315
     [Google Scholar]
  22. Tsukamura M. 1967; Identification of mycobacteria. Tubercle 48:311–338
     [Google Scholar]
  23. Tsukamura M. 1967; Differentiation of mycobacteria by utilization of nitrogen compounds and carbon sources. Am. Rev. Respir. Dis 95:307–310
     [Google Scholar]
  24. Tsukamura M., Tsukamura S. 1968; Differentiation of mycobacteria by susceptibility to nitrite and propylene glycol. Am. Rev. Respir. Dis 98:505–506
     [Google Scholar]
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Mycobacterium komossense sp. nov.
Int J Syst Evol Microbiol 29, 361 (1979); https://doi.org/10.1099/00207713-29-4-361
/content/journal/ijsem/10.1099/00207713-29-4-361
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